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dc.contributor.advisorHildebrand, John G.en_US
dc.contributor.authorStengl, Monika Anna-Helga.
dc.creatorStengl, Monika Anna-Helga.en_US
dc.date.accessioned2011-10-31T17:24:59Z
dc.date.available2011-10-31T17:24:59Z
dc.date.issued1990en_US
dc.identifier.urihttp://hdl.handle.net/10150/185008
dc.description.abstractSex-pheromone components released by Manduca sexta females are detected solely by male-specific olfactory receptor neurons that innervate long sensilla trichodea on the male antennae. To facilitate studies of the development and physiology of these receptor cells, I have produced primary in vitro cultures of cells dissociated from pupal male antennae. These cultures comprise several morphological types of cells, two of these cell types could be characterized immunocytochemically with a pair of monoclonal antibodies that were shown previously to recognize certain antigens in olfactory receptor neurons at defined stages of development. The good correlation between in vivo and in vitro expression of these antigens suggest that the immunocytochemically recognized cells are olfactory receptor neurons that follow at least partially their normal course of development in vitro. Patch-clamp studies revealed that the immunocytochemically recognized olfactory receptor neurons express three different kinds of Cs⁺-blockable K⁺ channels and at least one kind of Tetrodotoxin-blockable Na⁺ channel after three weeks in vitro. At least one channel, an unspecific cation channel, responds with higher frequency openings after stimulation with female pheromone-gland extracts in vitro. Thus it could be demonstrated for the first time that identifiable cultured insect olfactory receptor neurons differentiate in morphological and physiological terms and are able to respond to pheromones in vitro, providing an ideal model system for studies of primary sensory transduction in vitro.
dc.language.isoenen_US
dc.publisherThe University of Arizona.en_US
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en_US
dc.subjectBiologyen_US
dc.titleDevelopment of an in vitro model system to study primary sensory transduction mechanisms.en_US
dc.typetexten_US
dc.typeDissertation-Reproduction (electronic)en_US
dc.identifier.oclc708155142en_US
thesis.degree.grantorUniversity of Arizonaen_US
thesis.degree.leveldoctoralen_US
dc.contributor.committeememberBrower, Danny L.en_US
dc.contributor.committeememberLevine, Richard B.en_US
dc.contributor.committeememberLindell, Thomas J.en_US
dc.contributor.committeememberBurd, Gail D.en_US
dc.identifier.proquest9024518en_US
thesis.degree.disciplineMolecular and Cellular Biologyen_US
thesis.degree.disciplineGraduate Collegeen_US
thesis.degree.namePh.D.en_US
dc.description.noteThis item was digitized from a paper original and/or a microfilm copy. If you need higher-resolution images for any content in this item, please contact us at repository@u.library.arizona.edu.
dc.description.admin-noteOriginal file replaced with corrected file August 2023.
refterms.dateFOA2018-06-17T15:59:34Z
html.description.abstractSex-pheromone components released by Manduca sexta females are detected solely by male-specific olfactory receptor neurons that innervate long sensilla trichodea on the male antennae. To facilitate studies of the development and physiology of these receptor cells, I have produced primary in vitro cultures of cells dissociated from pupal male antennae. These cultures comprise several morphological types of cells, two of these cell types could be characterized immunocytochemically with a pair of monoclonal antibodies that were shown previously to recognize certain antigens in olfactory receptor neurons at defined stages of development. The good correlation between in vivo and in vitro expression of these antigens suggest that the immunocytochemically recognized cells are olfactory receptor neurons that follow at least partially their normal course of development in vitro. Patch-clamp studies revealed that the immunocytochemically recognized olfactory receptor neurons express three different kinds of Cs⁺-blockable K⁺ channels and at least one kind of Tetrodotoxin-blockable Na⁺ channel after three weeks in vitro. At least one channel, an unspecific cation channel, responds with higher frequency openings after stimulation with female pheromone-gland extracts in vitro. Thus it could be demonstrated for the first time that identifiable cultured insect olfactory receptor neurons differentiate in morphological and physiological terms and are able to respond to pheromones in vitro, providing an ideal model system for studies of primary sensory transduction in vitro.


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