Comparison and evaluation of two different methods to establish the cigarette smoke exposure mouse model of COPD

Persistent Link:
http://hdl.handle.net/10150/626192
Title:
Comparison and evaluation of two different methods to establish the cigarette smoke exposure mouse model of COPD
Author:
Shu, Jiaze; Li, Defu; Ouyang, Haiping; Huang, Junyi; Long, Zhen; Liang, Zhihao; Chen, Yuqin; Chen, Yiguan; Zheng, Qiuyu; Kuang, Meidan; Tang, Haiyang ( 0000-0001-9537-2891 ) ; Wang, Jian; Lu, Wenju
Affiliation:
Univ Arizona, Coll Med, Dept Physiol
Issue Date:
2017-11-13
Publisher:
NATURE PUBLISHING GROUP
Citation:
Comparison and evaluation of two different methods to establish the cigarette smoke exposure mouse model of COPD 2017, 7 (1) Scientific Reports
Journal:
Scientific Reports
Rights:
© The Author(s) 2017. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License.
Collection Information:
This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.
Abstract:
Animal model of cigarette smoke (CS) -induced chronic obstructive pulmonary disease (COPD) is the primary testing methodology for drug therapies and studies on pathogenic mechanisms of disease. However, researchers have rarely run simultaneous or side-by-side tests of whole-body and nose-only CS exposure in building their mouse models of COPD. We compared and evaluated these two different methods of CS exposure, plus airway Lipopolysaccharides (LPS) inhalation, in building our COPD mouse model. Compared with the control group, CS exposed mice showed significant increased inspiratory resistance, functional residual capacity, right ventricular hypertrophy index, and total cell count in BALF. Moreover, histological staining exhibited goblet cell hyperplasia, lung inflammation, thickening of smooth muscle layer on bronchia, and lung angiogenesis in both methods of CS exposure. Our data indicated that a viable mouse model of COPD can be established by combining the results from wholebody CS exposure, nose-only CS exposure, and airway LPS inhalation testing. However, in our study, we also found that, given the same amount of particulate intake, changes in right ventricular pressure and intimal thickening of pulmonary small artery are a little more serious in nose-only CS exposure method than changes in the whole-body CS exposure method.
ISSN:
2045-2322
PubMed ID:
29133824
DOI:
10.1038/s41598-017-15685-y
Version:
Final published version
Sponsors:
National Natural Science Foundation of China [81630004, 81470246, 81220108001, 81520108001]; Guangdong Department of Science and Technology of China [2016A030311020, 2016A030313606]; Guangzhou Department of Education Yangcheng Scholarship [12A001S]; Guangzhou Department of Education Scholarship [1201630095]; Guangzhou Department of Science and Technology [2014Y2-00167, 201607010358]; Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme, China
Additional Links:
http://www.nature.com/articles/s41598-017-15685-y

Full metadata record

DC FieldValue Language
dc.contributor.authorShu, Jiazeen
dc.contributor.authorLi, Defuen
dc.contributor.authorOuyang, Haipingen
dc.contributor.authorHuang, Junyien
dc.contributor.authorLong, Zhenen
dc.contributor.authorLiang, Zhihaoen
dc.contributor.authorChen, Yuqinen
dc.contributor.authorChen, Yiguanen
dc.contributor.authorZheng, Qiuyuen
dc.contributor.authorKuang, Meidanen
dc.contributor.authorTang, Haiyangen
dc.contributor.authorWang, Jianen
dc.contributor.authorLu, Wenjuen
dc.date.accessioned2017-12-05T16:10:30Z-
dc.date.available2017-12-05T16:10:30Z-
dc.date.issued2017-11-13-
dc.identifier.citationComparison and evaluation of two different methods to establish the cigarette smoke exposure mouse model of COPD 2017, 7 (1) Scientific Reportsen
dc.identifier.issn2045-2322-
dc.identifier.pmid29133824-
dc.identifier.doi10.1038/s41598-017-15685-y-
dc.identifier.urihttp://hdl.handle.net/10150/626192-
dc.description.abstractAnimal model of cigarette smoke (CS) -induced chronic obstructive pulmonary disease (COPD) is the primary testing methodology for drug therapies and studies on pathogenic mechanisms of disease. However, researchers have rarely run simultaneous or side-by-side tests of whole-body and nose-only CS exposure in building their mouse models of COPD. We compared and evaluated these two different methods of CS exposure, plus airway Lipopolysaccharides (LPS) inhalation, in building our COPD mouse model. Compared with the control group, CS exposed mice showed significant increased inspiratory resistance, functional residual capacity, right ventricular hypertrophy index, and total cell count in BALF. Moreover, histological staining exhibited goblet cell hyperplasia, lung inflammation, thickening of smooth muscle layer on bronchia, and lung angiogenesis in both methods of CS exposure. Our data indicated that a viable mouse model of COPD can be established by combining the results from wholebody CS exposure, nose-only CS exposure, and airway LPS inhalation testing. However, in our study, we also found that, given the same amount of particulate intake, changes in right ventricular pressure and intimal thickening of pulmonary small artery are a little more serious in nose-only CS exposure method than changes in the whole-body CS exposure method.en
dc.description.sponsorshipNational Natural Science Foundation of China [81630004, 81470246, 81220108001, 81520108001]; Guangdong Department of Science and Technology of China [2016A030311020, 2016A030313606]; Guangzhou Department of Education Yangcheng Scholarship [12A001S]; Guangzhou Department of Education Scholarship [1201630095]; Guangzhou Department of Science and Technology [2014Y2-00167, 201607010358]; Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme, Chinaen
dc.language.isoenen
dc.publisherNATURE PUBLISHING GROUPen
dc.relation.urlhttp://www.nature.com/articles/s41598-017-15685-yen
dc.rights© The Author(s) 2017. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License.en
dc.titleComparison and evaluation of two different methods to establish the cigarette smoke exposure mouse model of COPDen
dc.typeArticleen
dc.contributor.departmentUniv Arizona, Coll Med, Dept Physiolen
dc.identifier.journalScientific Reportsen
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en
dc.eprint.versionFinal published versionen

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