Expression patterns of conjunctival mucin 5AC and aquaporin 5 in response to acute dry eye stress

Persistent Link:
http://hdl.handle.net/10150/626191
Title:
Expression patterns of conjunctival mucin 5AC and aquaporin 5 in response to acute dry eye stress
Author:
Bhattacharya, Dhruva; Yu, Li; Wang, Mingwu ( 0000-0003-0259-3607 )
Affiliation:
Univ Arizona, Coll Med, Dept Ophthalmol & Vis Sci
Issue Date:
2017-11-07
Publisher:
PUBLIC LIBRARY SCIENCE
Citation:
Expression patterns of conjunctival mucin 5AC and aquaporin 5 in response to acute dry eye stress 2017, 12 (11):e0187188 PLOS ONE
Journal:
PLOS ONE
Rights:
© 2017 Bhattacharya et al. This is an open access article distributed under the terms of the Creative Commons Attribution License.
Collection Information:
This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.
Abstract:
The relationship between aquaporin (AQP) 5 and mucin (MUC) 5AC in the conjunctiva was investigated in response to acute dry eye (DE) stress. A mixed-mechanism rabbit DE model, in which the main lacrimal gland, Harderian gland, and nictitating membrane were resected, was further explored in this study. Conjunctival impression cytology specimens were harvested before excision (BE) and up to 3 months after excision (AE) in 8 (16 eyes) male New Zealand White rabbits, and immunoblotting was employed to assess the expression of AQP5 and MUC5AC. It was observed that AQP5 and MUC5AC showed a positive, synchronous expression pattern with progressive upregulation at protein level up to 2 months AE. At 3 months, the expression of both proteins decreased, but was still higher than that of BE. Such a synchronous relationship was further observed in mouse conjunctiva epithelium primary cells under hyperosmotic condition. Moreover, the co-immunoprecipitation of AQP5 and MUC5AC suggested a possible physical interaction between the two molecules. Our data indicates that conjunctival AQP5 and MUC5AC act synchronously in response to acute DE stress.
Note:
Open access journal.
ISSN:
1932-6203
PubMed ID:
29112967
DOI:
10.1371/journal.pone.0187188
Version:
Final published version
Sponsors:
Arizona Biomedical Research Commission Research [ADHS14-082988]; Department of Ophthalmology and Vision Science at University of Arizona
Additional Links:
http://dx.plos.org/10.1371/journal.pone.0187188

Full metadata record

DC FieldValue Language
dc.contributor.authorBhattacharya, Dhruvaen
dc.contributor.authorYu, Lien
dc.contributor.authorWang, Mingwuen
dc.date.accessioned2017-12-05T16:06:46Z-
dc.date.available2017-12-05T16:06:46Z-
dc.date.issued2017-11-07-
dc.identifier.citationExpression patterns of conjunctival mucin 5AC and aquaporin 5 in response to acute dry eye stress 2017, 12 (11):e0187188 PLOS ONEen
dc.identifier.issn1932-6203-
dc.identifier.pmid29112967-
dc.identifier.doi10.1371/journal.pone.0187188-
dc.identifier.urihttp://hdl.handle.net/10150/626191-
dc.description.abstractThe relationship between aquaporin (AQP) 5 and mucin (MUC) 5AC in the conjunctiva was investigated in response to acute dry eye (DE) stress. A mixed-mechanism rabbit DE model, in which the main lacrimal gland, Harderian gland, and nictitating membrane were resected, was further explored in this study. Conjunctival impression cytology specimens were harvested before excision (BE) and up to 3 months after excision (AE) in 8 (16 eyes) male New Zealand White rabbits, and immunoblotting was employed to assess the expression of AQP5 and MUC5AC. It was observed that AQP5 and MUC5AC showed a positive, synchronous expression pattern with progressive upregulation at protein level up to 2 months AE. At 3 months, the expression of both proteins decreased, but was still higher than that of BE. Such a synchronous relationship was further observed in mouse conjunctiva epithelium primary cells under hyperosmotic condition. Moreover, the co-immunoprecipitation of AQP5 and MUC5AC suggested a possible physical interaction between the two molecules. Our data indicates that conjunctival AQP5 and MUC5AC act synchronously in response to acute DE stress.en
dc.description.sponsorshipArizona Biomedical Research Commission Research [ADHS14-082988]; Department of Ophthalmology and Vision Science at University of Arizonaen
dc.language.isoenen
dc.publisherPUBLIC LIBRARY SCIENCEen
dc.relation.urlhttp://dx.plos.org/10.1371/journal.pone.0187188en
dc.rights© 2017 Bhattacharya et al. This is an open access article distributed under the terms of the Creative Commons Attribution License.en
dc.titleExpression patterns of conjunctival mucin 5AC and aquaporin 5 in response to acute dry eye stressen
dc.typeArticleen
dc.contributor.departmentUniv Arizona, Coll Med, Dept Ophthalmol & Vis Scien
dc.identifier.journalPLOS ONEen
dc.description.noteOpen access journal.en
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en
dc.eprint.versionFinal published versionen

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