The Effects of Developmental Nicotine Exposure on Hypoglossal Motoneuron Primary Dendrite and Soma Development in the Neonatal Rat

Persistent Link:
http://hdl.handle.net/10150/621005
Title:
The Effects of Developmental Nicotine Exposure on Hypoglossal Motoneuron Primary Dendrite and Soma Development in the Neonatal Rat
Author:
Gaddy, Joshua L.
Issue Date:
2016
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Nicotine from smoking or from other products containing nicotine has adverse effects on the fetus during pregnancy, such as respiratory problems. Our laboratory has previously shown that exposure to nicotine during development (DNE) alters hypoglossal motor neuron (XII MN) function, including decreased excitatory synaptic input, desensitized nicotinic acetylcholine receptors, increased input resistance, and differences in the precision and reliability of spike timing in XIIMNs. Evidence of DNE effects on XIIMN function prompted us to test the hypothesis that DNE will affect the development of primary dendrites and the soma. Brainstem slices were collected from neonates and motoneurons were filled with neurobiotin via whole-cell patch clamp. Filled cells were visualized with heavy metal intensified-3,3'-Diaminobenzidine (DAB) reaction. DAB-stained cells were analyzed using Neurolucida hardware and software. On average, the maximum soma diameter of more rostral XIIMNs was larger than that in more caudal cells. Also, caudal XIIMNs had more primary nodes than rostral XIIMNs, and there was a significant treatment effect on minimum soma diameter (Control, 13.76 ± 0.71 µm; DNE, 18.09 ± 1.22 µm). The results from this study uncovered potential effects of nicotine on XIIMNs found in rostral and caudal regions of the hypoglossal nucleus.
Type:
text; Electronic Thesis
Keywords:
Hypoglossal; Motor Neurons; Nicotine; Respiratory; Soma; Cellular and Molecular Medicine; Dendrite
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College; Cellular and Molecular Medicine
Degree Grantor:
University of Arizona
Advisor:
Fregosi, Ralph F.

Full metadata record

DC FieldValue Language
dc.language.isoen_USen
dc.titleThe Effects of Developmental Nicotine Exposure on Hypoglossal Motoneuron Primary Dendrite and Soma Development in the Neonatal Raten_US
dc.creatorGaddy, Joshua L.en
dc.contributor.authorGaddy, Joshua L.en
dc.date.issued2016-
dc.publisherThe University of Arizona.en
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en
dc.description.abstractNicotine from smoking or from other products containing nicotine has adverse effects on the fetus during pregnancy, such as respiratory problems. Our laboratory has previously shown that exposure to nicotine during development (DNE) alters hypoglossal motor neuron (XII MN) function, including decreased excitatory synaptic input, desensitized nicotinic acetylcholine receptors, increased input resistance, and differences in the precision and reliability of spike timing in XIIMNs. Evidence of DNE effects on XIIMN function prompted us to test the hypothesis that DNE will affect the development of primary dendrites and the soma. Brainstem slices were collected from neonates and motoneurons were filled with neurobiotin via whole-cell patch clamp. Filled cells were visualized with heavy metal intensified-3,3'-Diaminobenzidine (DAB) reaction. DAB-stained cells were analyzed using Neurolucida hardware and software. On average, the maximum soma diameter of more rostral XIIMNs was larger than that in more caudal cells. Also, caudal XIIMNs had more primary nodes than rostral XIIMNs, and there was a significant treatment effect on minimum soma diameter (Control, 13.76 ± 0.71 µm; DNE, 18.09 ± 1.22 µm). The results from this study uncovered potential effects of nicotine on XIIMNs found in rostral and caudal regions of the hypoglossal nucleus.en
dc.typetexten
dc.typeElectronic Thesisen
dc.subjectHypoglossalen
dc.subjectMotor Neuronsen
dc.subjectNicotineen
dc.subjectRespiratoryen
dc.subjectSomaen
dc.subjectCellular and Molecular Medicineen
dc.subjectDendriteen
thesis.degree.nameM.S.en
thesis.degree.levelmastersen
thesis.degree.disciplineGraduate Collegeen
thesis.degree.disciplineCellular and Molecular Medicineen
thesis.degree.grantorUniversity of Arizonaen
dc.contributor.advisorFregosi, Ralph F.en
dc.contributor.committeememberLevine, Richard B.en
dc.contributor.committeememberSt. John, Paul A.en
dc.contributor.committeememberAmerongen, Helenen
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