Cellular Architecture Regulates Collective Calcium Signaling and Cell Contractility

Persistent Link:
http://hdl.handle.net/10150/618963
Title:
Cellular Architecture Regulates Collective Calcium Signaling and Cell Contractility
Author:
Sun, Jian ( 0000-0001-8539-6590 ) ; Hoying, James B.; Deymier, Pierre A.; Zhang, Donna D.; Wong, Pak Kin
Affiliation:
Univ Arizona, Dept Aerosp & Mech Engn; Univ Arizona, Dept Mat Sci & Engn; Univ Arizona, Dept Pharmacol & Toxicol
Issue Date:
2016-05-19
Publisher:
Public Library of Science
Citation:
Cellular Architecture Regulates Collective Calcium Signaling and Cell Contractility 2016, 12 (5):e1004955 PLOS Computational Biology
Journal:
PLOS Computational Biology
Rights:
© 2016 Sun et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Collection Information:
This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.
Abstract:
A key feature of multicellular systems is the ability of cells to function collectively in response to external stimuli. However, the mechanisms of intercellular cell signaling and their functional implications in diverse vascular structures are poorly understood. Using a combination of computational modeling and plasma lithography micropatterning, we investigate the roles of structural arrangement of endothelial cells in collective calcium signaling and cell contractility. Under histamine stimulation, endothelial cells in self-assembled and microengineered networks, but not individual cells and monolayers, exhibit calcium oscillations. Micropatterning, pharmacological inhibition, and computational modeling reveal that the calcium oscillation depends on the number of neighboring cells coupled via gap junctional intercellular communication, providing a mechanistic basis of the architecture-dependent calcium signaling. Furthermore, the calcium oscillation attenuates the histamine-induced cytoskeletal reorganization and cell contraction, resulting in differential cell responses in an architecture-dependent manner. Taken together, our results suggest that endothelial cells can sense and respond to chemical stimuli according to the vascular architecture via collective calcium signaling.
Note:
Open Access Journal
ISSN:
1553-7358
DOI:
10.1371/journal.pcbi.1004955
Version:
Final published version
Sponsors:
National Institutes of Health Director's New Innovator Award [DP2OD007161]; James S. McDonnell Foundation
Additional Links:
http://dx.plos.org/10.1371/journal.pcbi.1004955

Full metadata record

DC FieldValue Language
dc.contributor.authorSun, Jianen
dc.contributor.authorHoying, James B.en
dc.contributor.authorDeymier, Pierre A.en
dc.contributor.authorZhang, Donna D.en
dc.contributor.authorWong, Pak Kinen
dc.date.accessioned2016-08-27T01:07:34Z-
dc.date.available2016-08-27T01:07:34Z-
dc.date.issued2016-05-19-
dc.identifier.citationCellular Architecture Regulates Collective Calcium Signaling and Cell Contractility 2016, 12 (5):e1004955 PLOS Computational Biologyen
dc.identifier.issn1553-7358-
dc.identifier.doi10.1371/journal.pcbi.1004955-
dc.identifier.urihttp://hdl.handle.net/10150/618963-
dc.description.abstractA key feature of multicellular systems is the ability of cells to function collectively in response to external stimuli. However, the mechanisms of intercellular cell signaling and their functional implications in diverse vascular structures are poorly understood. Using a combination of computational modeling and plasma lithography micropatterning, we investigate the roles of structural arrangement of endothelial cells in collective calcium signaling and cell contractility. Under histamine stimulation, endothelial cells in self-assembled and microengineered networks, but not individual cells and monolayers, exhibit calcium oscillations. Micropatterning, pharmacological inhibition, and computational modeling reveal that the calcium oscillation depends on the number of neighboring cells coupled via gap junctional intercellular communication, providing a mechanistic basis of the architecture-dependent calcium signaling. Furthermore, the calcium oscillation attenuates the histamine-induced cytoskeletal reorganization and cell contraction, resulting in differential cell responses in an architecture-dependent manner. Taken together, our results suggest that endothelial cells can sense and respond to chemical stimuli according to the vascular architecture via collective calcium signaling.en
dc.description.sponsorshipNational Institutes of Health Director's New Innovator Award [DP2OD007161]; James S. McDonnell Foundationen
dc.language.isoenen
dc.publisherPublic Library of Scienceen
dc.relation.urlhttp://dx.plos.org/10.1371/journal.pcbi.1004955en
dc.rights© 2016 Sun et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are crediteden
dc.titleCellular Architecture Regulates Collective Calcium Signaling and Cell Contractilityen
dc.typeArticleen
dc.contributor.departmentUniv Arizona, Dept Aerosp & Mech Engnen
dc.contributor.departmentUniv Arizona, Dept Mat Sci & Engnen
dc.contributor.departmentUniv Arizona, Dept Pharmacol & Toxicolen
dc.identifier.journalPLOS Computational Biologyen
dc.description.noteOpen Access Journalen
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en
dc.eprint.versionFinal published versionen
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