Sequencing, de novo assembly and annotation of a pink bollworm larval midgut transcriptome

Persistent Link:
http://hdl.handle.net/10150/618722
Title:
Sequencing, de novo assembly and annotation of a pink bollworm larval midgut transcriptome
Author:
Tassone, Erica E.; Zastrow-Hayes, Gina; Mathis, John; Nelson, Mark E.; Wu, Gusui; Flexner, J. Lindsey; Carrière, Yves; Tabashnik, Bruce E.; Fabrick, Jeffrey A.
Affiliation:
Univ Arizona, Dept Entomol
Issue Date:
2016-06-22
Publisher:
BioMed Central
Citation:
Sequencing, de novo assembly and annotation of a pink bollworm larval midgut transcriptome 2016, 5 (1) GigaScience
Journal:
GigaScience
Rights:
© 2016 The Author(s). Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/).
Collection Information:
This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.
Abstract:
Background: The pink bollworm Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae) is one of the world's most important pests of cotton. Insecticide sprays and transgenic cotton producing toxins of the bacterium Bacillus thuringiensis (Bt) are currently used to manage this pest. Bt toxins kill susceptible insects by specifically binding to and destroying midgut cells, but they are not toxic to most other organisms. Pink bollworm is useful as a model for understanding insect responses to Bt toxins, yet advances in understanding at the molecular level have been limited because basic genomic information is lacking for this cosmopolitan pest. Here, we have sequenced, de novo assembled and annotated a comprehensive larval midgut transcriptome from a susceptible strain of pink bollworm. Findings: A de novo transcriptome assembly for the midgut of P. gossypiella was generated containing 46,458 transcripts (average length of 770 bp) derived from 39,874 unigenes. The size of the transcriptome is similar to published midgut transcriptomes of other Lepidoptera and includes up to 91 % annotated contigs. The dataset is publicly available in NCBI and GigaDB as a resource for researchers. Conclusions: Foundational knowledge of protein-coding genes from the pink bollworm midgut is critical for understanding how this important insect pest functions. The transcriptome data presented here represent the first large-scale molecular resource for this species, and may be used for deciphering relevant midgut proteins critical for xenobiotic detoxification, nutrient digestion and allocation, as well as for the discovery of protein receptors important for Bt intoxication.
Note:
Open Access Journal
ISSN:
2047-217X
PubMed ID:
27333791
DOI:
10.1186/s13742-016-0130-9
Keywords:
Pectinophora gossypiella; Pink bollworm; RNA-Seq; Transcriptome; Midgut; Bacillus thuringiensis
Version:
Final published version
Sponsors:
USDA-ARS; DuPont-Pioneer [58-3K95-4-1666]
Additional Links:
http://gigascience.biomedcentral.com/articles/10.1186/s13742-016-0130-9

Full metadata record

DC FieldValue Language
dc.contributor.authorTassone, Erica E.en
dc.contributor.authorZastrow-Hayes, Ginaen
dc.contributor.authorMathis, Johnen
dc.contributor.authorNelson, Mark E.en
dc.contributor.authorWu, Gusuien
dc.contributor.authorFlexner, J. Lindseyen
dc.contributor.authorCarrière, Yvesen
dc.contributor.authorTabashnik, Bruce E.en
dc.contributor.authorFabrick, Jeffrey A.en
dc.date.accessioned2016-08-24T01:14:08Z-
dc.date.available2016-08-24T01:14:08Z-
dc.date.issued2016-06-22-
dc.identifier.citationSequencing, de novo assembly and annotation of a pink bollworm larval midgut transcriptome 2016, 5 (1) GigaScienceen
dc.identifier.issn2047-217X-
dc.identifier.pmid27333791-
dc.identifier.doi10.1186/s13742-016-0130-9-
dc.identifier.urihttp://hdl.handle.net/10150/618722-
dc.description.abstractBackground: The pink bollworm Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae) is one of the world's most important pests of cotton. Insecticide sprays and transgenic cotton producing toxins of the bacterium Bacillus thuringiensis (Bt) are currently used to manage this pest. Bt toxins kill susceptible insects by specifically binding to and destroying midgut cells, but they are not toxic to most other organisms. Pink bollworm is useful as a model for understanding insect responses to Bt toxins, yet advances in understanding at the molecular level have been limited because basic genomic information is lacking for this cosmopolitan pest. Here, we have sequenced, de novo assembled and annotated a comprehensive larval midgut transcriptome from a susceptible strain of pink bollworm. Findings: A de novo transcriptome assembly for the midgut of P. gossypiella was generated containing 46,458 transcripts (average length of 770 bp) derived from 39,874 unigenes. The size of the transcriptome is similar to published midgut transcriptomes of other Lepidoptera and includes up to 91 % annotated contigs. The dataset is publicly available in NCBI and GigaDB as a resource for researchers. Conclusions: Foundational knowledge of protein-coding genes from the pink bollworm midgut is critical for understanding how this important insect pest functions. The transcriptome data presented here represent the first large-scale molecular resource for this species, and may be used for deciphering relevant midgut proteins critical for xenobiotic detoxification, nutrient digestion and allocation, as well as for the discovery of protein receptors important for Bt intoxication.en
dc.description.sponsorshipUSDA-ARS; DuPont-Pioneer [58-3K95-4-1666]en
dc.language.isoenen
dc.publisherBioMed Centralen
dc.relation.urlhttp://gigascience.biomedcentral.com/articles/10.1186/s13742-016-0130-9en
dc.rights© 2016 The Author(s). Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/).en
dc.subjectPectinophora gossypiellaen
dc.subjectPink bollwormen
dc.subjectRNA-Seqen
dc.subjectTranscriptomeen
dc.subjectMidguten
dc.subjectBacillus thuringiensisen
dc.titleSequencing, de novo assembly and annotation of a pink bollworm larval midgut transcriptomeen
dc.typeArticleen
dc.contributor.departmentUniv Arizona, Dept Entomolen
dc.identifier.journalGigaScienceen
dc.description.noteOpen Access Journalen
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en
dc.eprint.versionFinal published versionen

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