Longitudinal Effects of Embryonic Exposure to Cocaine on Morphology, Cardiovascular Physiology, and Behavior in Zebrafish

Persistent Link:
http://hdl.handle.net/10150/618721
Title:
Longitudinal Effects of Embryonic Exposure to Cocaine on Morphology, Cardiovascular Physiology, and Behavior in Zebrafish
Author:
Mersereau, Eric; Boyle, Cody; Poitra, Shelby; Espinoza, Ana; Seiler, Joclyn; Longie, Robert; Delvo, Lisa; Szarkowski, Megan; Maliske, Joshua; Chalmers, Sarah; Darland, Diane; Darland, Tristan
Affiliation:
Univ Arizona, Dept Ecol & Evolutionary Biol
Issue Date:
2016-05-31
Publisher:
MDPI AG
Citation:
Longitudinal Effects of Embryonic Exposure to Cocaine on Morphology, Cardiovascular Physiology, and Behavior in Zebrafish 2016, 17 (6):847 International Journal of Molecular Sciences
Journal:
International Journal of Molecular Sciences
Rights:
© 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
Collection Information:
This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.
Abstract:
A sizeable portion of the societal drain from cocaine abuse results from the complications of in utero drug exposure. Because of challenges in using humans and mammalian model organisms as test subjects, much debate remains about the impact of in utero cocaine exposure. Zebrafish offer a number of advantages as a model in longitudinal toxicology studies and are quite sensitive physiologically and behaviorally to cocaine. In this study, we have used zebrafish to model the effects of embryonic pre-exposure to cocaine on development and on subsequent cardiovascular physiology and cocaine-induced conditioned place preference (CPP) in longitudinal adults. Larval fish showed a progressive decrease in telencephalic size with increased doses of cocaine. These treated larvae also showed a dose dependent response in heart rate that persisted 24 h after drug cessation. Embryonic cocaine exposure had little effect on overall health of longitudinal adults, but subtle changes in cardiovascular physiology were seen including decreased sensitivity to isoproterenol and increased sensitivity to cocaine. These longitudinal adult fish also showed an embryonic dose-dependent change in CPP behavior, suggesting an increased sensitivity. These studies clearly show that pre-exposure during embryonic development affects subsequent cocaine sensitivity in longitudinal adults.
Note:
Open Access Journal
ISSN:
1422-0067
DOI:
10.3390/ijms17060847
Keywords:
cocaine; zebrafish; cardiovascular physiology; behavior
Version:
Final published version
Sponsors:
University of North Dakota (UND); Department of Pathology at the UND School of Medicine and Health Sciences; National Science Foundation (NSF) REU Site [0851869]; National Institutes of Health (NIH) [P20GM103442/P20RR016471]; Indian Health Service (IHS) [U261HS0045-04-01]; NIH
Additional Links:
http://www.mdpi.com/1422-0067/17/6/847

Full metadata record

DC FieldValue Language
dc.contributor.authorMersereau, Ericen
dc.contributor.authorBoyle, Codyen
dc.contributor.authorPoitra, Shelbyen
dc.contributor.authorEspinoza, Anaen
dc.contributor.authorSeiler, Joclynen
dc.contributor.authorLongie, Roberten
dc.contributor.authorDelvo, Lisaen
dc.contributor.authorSzarkowski, Meganen
dc.contributor.authorMaliske, Joshuaen
dc.contributor.authorChalmers, Sarahen
dc.contributor.authorDarland, Dianeen
dc.contributor.authorDarland, Tristanen
dc.date.accessioned2016-08-24T01:05:36Z-
dc.date.available2016-08-24T01:05:36Z-
dc.date.issued2016-05-31-
dc.identifier.citationLongitudinal Effects of Embryonic Exposure to Cocaine on Morphology, Cardiovascular Physiology, and Behavior in Zebrafish 2016, 17 (6):847 International Journal of Molecular Sciencesen
dc.identifier.issn1422-0067-
dc.identifier.doi10.3390/ijms17060847-
dc.identifier.urihttp://hdl.handle.net/10150/618721-
dc.description.abstractA sizeable portion of the societal drain from cocaine abuse results from the complications of in utero drug exposure. Because of challenges in using humans and mammalian model organisms as test subjects, much debate remains about the impact of in utero cocaine exposure. Zebrafish offer a number of advantages as a model in longitudinal toxicology studies and are quite sensitive physiologically and behaviorally to cocaine. In this study, we have used zebrafish to model the effects of embryonic pre-exposure to cocaine on development and on subsequent cardiovascular physiology and cocaine-induced conditioned place preference (CPP) in longitudinal adults. Larval fish showed a progressive decrease in telencephalic size with increased doses of cocaine. These treated larvae also showed a dose dependent response in heart rate that persisted 24 h after drug cessation. Embryonic cocaine exposure had little effect on overall health of longitudinal adults, but subtle changes in cardiovascular physiology were seen including decreased sensitivity to isoproterenol and increased sensitivity to cocaine. These longitudinal adult fish also showed an embryonic dose-dependent change in CPP behavior, suggesting an increased sensitivity. These studies clearly show that pre-exposure during embryonic development affects subsequent cocaine sensitivity in longitudinal adults.en
dc.description.sponsorshipUniversity of North Dakota (UND); Department of Pathology at the UND School of Medicine and Health Sciences; National Science Foundation (NSF) REU Site [0851869]; National Institutes of Health (NIH) [P20GM103442/P20RR016471]; Indian Health Service (IHS) [U261HS0045-04-01]; NIHen
dc.language.isoenen
dc.publisherMDPI AGen
dc.relation.urlhttp://www.mdpi.com/1422-0067/17/6/847en
dc.rights© 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).en
dc.subjectcocaineen
dc.subjectzebrafishen
dc.subjectcardiovascular physiologyen
dc.subjectbehavioren
dc.titleLongitudinal Effects of Embryonic Exposure to Cocaine on Morphology, Cardiovascular Physiology, and Behavior in Zebrafishen
dc.typeArticleen
dc.contributor.departmentUniv Arizona, Dept Ecol & Evolutionary Biolen
dc.identifier.journalInternational Journal of Molecular Sciencesen
dc.description.noteOpen Access Journalen
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en
dc.eprint.versionFinal published versionen
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