Comparative Proteomic Analysis of Cotton Fiber Development and Protein Extraction Method Comparison in Late Stage Fibers

Persistent Link:
http://hdl.handle.net/10150/618719
Title:
Comparative Proteomic Analysis of Cotton Fiber Development and Protein Extraction Method Comparison in Late Stage Fibers
Author:
Mujahid, Hana; Pendarvis, Ken; Reddy, Joseph; Nallamilli, Babi; Reddy, K.; Nanduri, Bindu; Peng, Zhaohua
Affiliation:
Univ Arizona, Coll Agr & Life Sci
Issue Date:
2016-02-03
Publisher:
MDPI AG
Citation:
Comparative Proteomic Analysis of Cotton Fiber Development and Protein Extraction Method Comparison in Late Stage Fibers 2016, 4 (1):7 Proteomes
Journal:
Proteomes
Rights:
© 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
Collection Information:
This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.
Abstract:
The distinct stages of cotton fiber development and maturation serve as a single-celled model for studying the molecular mechanisms of plant cell elongation, cell wall development and cellulose biosynthesis. However, this model system of plant cell development is compromised for proteomic studies due to a lack of an efficient protein extraction method during the later stages of fiber development, because of a recalcitrant cell wall and the presence of abundant phenolic compounds. Here, we compared the quality and quantities of proteins extracted from 25 dpa (days post anthesis) fiber with multiple protein extraction methods and present a comprehensive quantitative proteomic study of fiber development from 10 dpa to 25 dpa. Comparative analysis using a label-free quantification method revealed 287 differentially-expressed proteins in the 10 dpa to 25 dpa fiber developmental period. Proteins involved in cell wall metabolism and regulation, cytoskeleton development and carbohydrate metabolism among other functional categories in four fiber developmental stages were identified. Our studies provide protocols for protein extraction from maturing fiber tissues for mass spectrometry analysis and expand knowledge of the proteomic profile of cotton fiber development.
Note:
Open Access Journal
ISSN:
2227-7382
DOI:
10.3390/proteomes4010007
Keywords:
cotton; fiber elongation; cell wall; comparative proteomics; label-free
Version:
Final published version
Additional Links:
http://www.mdpi.com/2227-7382/4/1/7

Full metadata record

DC FieldValue Language
dc.contributor.authorMujahid, Hanaen
dc.contributor.authorPendarvis, Kenen
dc.contributor.authorReddy, Josephen
dc.contributor.authorNallamilli, Babien
dc.contributor.authorReddy, K.en
dc.contributor.authorNanduri, Binduen
dc.contributor.authorPeng, Zhaohuaen
dc.date.accessioned2016-08-24T00:58:01Z-
dc.date.available2016-08-24T00:58:01Z-
dc.date.issued2016-02-03-
dc.identifier.citationComparative Proteomic Analysis of Cotton Fiber Development and Protein Extraction Method Comparison in Late Stage Fibers 2016, 4 (1):7 Proteomesen
dc.identifier.issn2227-7382-
dc.identifier.doi10.3390/proteomes4010007-
dc.identifier.urihttp://hdl.handle.net/10150/618719-
dc.description.abstractThe distinct stages of cotton fiber development and maturation serve as a single-celled model for studying the molecular mechanisms of plant cell elongation, cell wall development and cellulose biosynthesis. However, this model system of plant cell development is compromised for proteomic studies due to a lack of an efficient protein extraction method during the later stages of fiber development, because of a recalcitrant cell wall and the presence of abundant phenolic compounds. Here, we compared the quality and quantities of proteins extracted from 25 dpa (days post anthesis) fiber with multiple protein extraction methods and present a comprehensive quantitative proteomic study of fiber development from 10 dpa to 25 dpa. Comparative analysis using a label-free quantification method revealed 287 differentially-expressed proteins in the 10 dpa to 25 dpa fiber developmental period. Proteins involved in cell wall metabolism and regulation, cytoskeleton development and carbohydrate metabolism among other functional categories in four fiber developmental stages were identified. Our studies provide protocols for protein extraction from maturing fiber tissues for mass spectrometry analysis and expand knowledge of the proteomic profile of cotton fiber development.en
dc.language.isoenen
dc.publisherMDPI AGen
dc.relation.urlhttp://www.mdpi.com/2227-7382/4/1/7en
dc.rights© 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).en
dc.subjectcottonen
dc.subjectfiber elongationen
dc.subjectcell wallen
dc.subjectcomparative proteomicsen
dc.subjectlabel-freeen
dc.titleComparative Proteomic Analysis of Cotton Fiber Development and Protein Extraction Method Comparison in Late Stage Fibersen
dc.typeArticleen
dc.contributor.departmentUniv Arizona, Coll Agr & Life Scien
dc.identifier.journalProteomesen
dc.description.noteOpen Access Journalen
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en
dc.eprint.versionFinal published versionen
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