Biodistribution and Lymphatic Tracking of the Main Neurotoxin of Micrurus fulvius Venom by Molecular Imaging

Persistent Link:
http://hdl.handle.net/10150/614979
Title:
Biodistribution and Lymphatic Tracking of the Main Neurotoxin of Micrurus fulvius Venom by Molecular Imaging
Author:
Vergara, Irene; Castillo, Erick; Romero-Piña, Mario; Torres-Viquez, Itzel; Paniagua, Dayanira; Boyer, Leslie; Alagón, Alejandro; Medina, Luis
Affiliation:
Univ Arizona, Coll Med, Venom Immunochem Pharmacol & Emergency Response V
Issue Date:
2016-03-26
Publisher:
MDPI AG
Citation:
Biodistribution and Lymphatic Tracking of the Main Neurotoxin of Micrurus fulvius Venom by Molecular Imaging 2016, 8 (4):85 Toxins
Journal:
Toxins
Rights:
© 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
Collection Information:
This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.
Abstract:
The venom of the Eastern coral snake Micrurus fulvius can cause respiratory paralysis in the bitten patient, which is attributable to -neurotoxins (-NTx). The aim of this work was to study the biodistribution and lymphatic tracking by molecular imaging of the main -NTx of M. fulvius venom. -NTx was bioconjugated with the chelator diethylenetriaminepenta-acetic acid (DTPA) and radiolabeled with the radionuclide Gallium-67. Radiolabeling efficiency was 60%-78%; radiochemical purity 92%; and stability at 48 h 85%. The median lethal dose (LD50) and PLA(2) activity of bioconjugated -NTx decreased 3 and 2.5 times, respectively, in comparison with native -NTx. The immune recognition by polyclonal antibodies decreased 10 times. Biodistribution of -NTx-DTPA-Ga-67 in rats showed increased uptake in popliteal, lumbar nodes and kidneys that was not observed with Ga-67-free. Accumulation in organs at 24 h was less than 1%, except for kidneys, where the average was 3.7%. The inoculation site works as a depot, since 10% of the initial dose of -NTx-DTPA-Ga-67 remains there for up to 48 h. This work clearly demonstrates the lymphatic system participation in the biodistribution of -NTx-DTPA-Ga-67. Our approach could be applied to analyze the role of the lymphatic system in snakebite for a better understanding of envenoming.
ISSN:
2072-6651
DOI:
10.3390/toxins8040085
Keywords:
coral snake; neurotoxin; lymphatic absorption; molecular imaging; radiolabeling
Version:
Final published version
Additional Links:
http://www.mdpi.com/2072-6651/8/4/85

Full metadata record

DC FieldValue Language
dc.contributor.authorVergara, Ireneen
dc.contributor.authorCastillo, Ericken
dc.contributor.authorRomero-Piña, Marioen
dc.contributor.authorTorres-Viquez, Itzelen
dc.contributor.authorPaniagua, Dayaniraen
dc.contributor.authorBoyer, Leslieen
dc.contributor.authorAlagón, Alejandroen
dc.contributor.authorMedina, Luisen
dc.date.accessioned2016-06-29T00:31:03Z-
dc.date.available2016-06-29T00:31:03Z-
dc.date.issued2016-03-26-
dc.identifier.citationBiodistribution and Lymphatic Tracking of the Main Neurotoxin of Micrurus fulvius Venom by Molecular Imaging 2016, 8 (4):85 Toxinsen
dc.identifier.issn2072-6651-
dc.identifier.doi10.3390/toxins8040085-
dc.identifier.urihttp://hdl.handle.net/10150/614979-
dc.description.abstractThe venom of the Eastern coral snake Micrurus fulvius can cause respiratory paralysis in the bitten patient, which is attributable to -neurotoxins (-NTx). The aim of this work was to study the biodistribution and lymphatic tracking by molecular imaging of the main -NTx of M. fulvius venom. -NTx was bioconjugated with the chelator diethylenetriaminepenta-acetic acid (DTPA) and radiolabeled with the radionuclide Gallium-67. Radiolabeling efficiency was 60%-78%; radiochemical purity 92%; and stability at 48 h 85%. The median lethal dose (LD50) and PLA(2) activity of bioconjugated -NTx decreased 3 and 2.5 times, respectively, in comparison with native -NTx. The immune recognition by polyclonal antibodies decreased 10 times. Biodistribution of -NTx-DTPA-Ga-67 in rats showed increased uptake in popliteal, lumbar nodes and kidneys that was not observed with Ga-67-free. Accumulation in organs at 24 h was less than 1%, except for kidneys, where the average was 3.7%. The inoculation site works as a depot, since 10% of the initial dose of -NTx-DTPA-Ga-67 remains there for up to 48 h. This work clearly demonstrates the lymphatic system participation in the biodistribution of -NTx-DTPA-Ga-67. Our approach could be applied to analyze the role of the lymphatic system in snakebite for a better understanding of envenoming.en
dc.language.isoenen
dc.publisherMDPI AGen
dc.relation.urlhttp://www.mdpi.com/2072-6651/8/4/85en
dc.rights© 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).en
dc.subjectcoral snakeen
dc.subjectneurotoxinen
dc.subjectlymphatic absorptionen
dc.subjectmolecular imagingen
dc.subjectradiolabelingen
dc.titleBiodistribution and Lymphatic Tracking of the Main Neurotoxin of Micrurus fulvius Venom by Molecular Imagingen
dc.typeArticleen
dc.contributor.departmentUniv Arizona, Coll Med, Venom Immunochem Pharmacol & Emergency Response Ven
dc.identifier.journalToxinsen
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en
dc.eprint.versionFinal published versionen
All Items in UA Campus Repository are protected by copyright, with all rights reserved, unless otherwise indicated.