Influence of periparturient and postpartum diets on rumen methanogen communities in three breeds of primiparous dairy cows

Persistent Link:
http://hdl.handle.net/10150/614742
Title:
Influence of periparturient and postpartum diets on rumen methanogen communities in three breeds of primiparous dairy cows
Author:
Cersosimo, Laura M.; Bainbridge, Melissa L.; Kraft, Jana; Wright, André-Denis G.
Affiliation:
Univ Arizona, Sch Anim & Comparat Biomed Sci
Issue Date:
2016-05-04
Publisher:
BIOMED CENTRAL LTD
Citation:
Influence of periparturient and postpartum diets on rumen methanogen communities in three breeds of primiparous dairy cows 2016, 16 (1) BMC Microbiology
Journal:
BMC Microbiology
Rights:
© 2016 Cersosimo et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/).
Collection Information:
This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.
Abstract:
Background: Enteric methane from rumen methanogens is responsible for 25.9 % of total methane emissions in the United States. Rumen methanogens also contribute to decreased animal feed efficiency. For methane mitigation strategies to be successful, it is important to establish which factors influence the rumen methanogen community and rumen volatile fatty acids (VFA). In the present study, we used next-generation sequencing to determine if dairy breed and/or days in milk (DIM) (high-fiber periparturient versus high-starch postpartum diets) affect the rumen environment and methanogen community of primiparous Holstein, Jersey, and Holstein-Jersey crossbreeds. Results: When the 16S rRNA gene sequences were processed and assigned to operational taxonomic units (OTU), a core methanogen community was identified, consisting of Methanobrevibacter (Mbr.) smithii, Mbr. thaueri, Mbr. ruminantium, and Mbr. millerae. The 16S rRNA gene sequence reads clustered at 3 DIM, but not by breed. At 3 DIM, the mean % abundance of Mbr. thaueri was lower in Jerseys (26.9 %) and higher in Holsteins (30.7 %) and Holstein-Jersey crossbreeds (30.3 %) (P < 0.001). The molar concentrations of total VFA were higher at 3 DIM than at 93, 183, and 273 DIM, whereas the molar proportions of propionate were increased at 3 and 93 DIM, relative to 183 and 273 DIM. Rumen methanogen densities, distributions of the Mbr. species, and VFA molar proportions did not differ by breed. Conclusions: The data from the present study suggest that a core methanogen community is present among dairy breeds, through out a lactation. Furthermore, the methanogen communities were more influenced by DIM and the breed by DIM interactions than breed differences.
ISSN:
1471-2180
DOI:
10.1186/s12866-016-0694-7
Keywords:
Archaea; Diversity; Holstein-Jersey; Jersey; 16S rRNA gene; Volatile fatty acids
Version:
Final published version
Sponsors:
The author's would like to acknowledge the USDA Hatch Grant (VT-H01801) and the UVM Dairy Center of Excellence as funding sources for animals and laboratory supplies. Furthermore, the Agriculture and Food Research Initiative Competitive Grant no. 2014-67016-21791 from the USDA National Institute of Food and Agriculture supported the VFA and feed analyses. We would like to thank Dr. Benoit St-Pierre of South Dakota State University for assistance with bioinformatics and Kurt Cotanch of the William H. Miner Institute for assistance with VFA analyses. We would also like to thank the Paul Miller Research Farm staff (Matt Boudette, Scott Shumway, Doug Watkin) for their on-farm maintenance and assistance. Lastly, we would like to thank Columbia University undergraduate student, Ryan C. Noyes, and the UVM undergraduate students (Pamela Bay, Katherine Boucher, Dylan Devino, Michael Eldredge, Samantha Frawley, Emma Hurley, Anne Kaufman, Danielle Semick, Mallory Sullivan, Sarah Zeger) who helped with calving, sampling, and milking.
Additional Links:
http://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-016-0694-7

Full metadata record

DC FieldValue Language
dc.contributor.authorCersosimo, Laura M.en
dc.contributor.authorBainbridge, Melissa L.en
dc.contributor.authorKraft, Janaen
dc.contributor.authorWright, André-Denis G.en
dc.date.accessioned2016-06-24T22:58:25Z-
dc.date.available2016-06-24T22:58:25Z-
dc.date.issued2016-05-04-
dc.identifier.citationInfluence of periparturient and postpartum diets on rumen methanogen communities in three breeds of primiparous dairy cows 2016, 16 (1) BMC Microbiologyen
dc.identifier.issn1471-2180-
dc.identifier.doi10.1186/s12866-016-0694-7-
dc.identifier.urihttp://hdl.handle.net/10150/614742-
dc.description.abstractBackground: Enteric methane from rumen methanogens is responsible for 25.9 % of total methane emissions in the United States. Rumen methanogens also contribute to decreased animal feed efficiency. For methane mitigation strategies to be successful, it is important to establish which factors influence the rumen methanogen community and rumen volatile fatty acids (VFA). In the present study, we used next-generation sequencing to determine if dairy breed and/or days in milk (DIM) (high-fiber periparturient versus high-starch postpartum diets) affect the rumen environment and methanogen community of primiparous Holstein, Jersey, and Holstein-Jersey crossbreeds. Results: When the 16S rRNA gene sequences were processed and assigned to operational taxonomic units (OTU), a core methanogen community was identified, consisting of Methanobrevibacter (Mbr.) smithii, Mbr. thaueri, Mbr. ruminantium, and Mbr. millerae. The 16S rRNA gene sequence reads clustered at 3 DIM, but not by breed. At 3 DIM, the mean % abundance of Mbr. thaueri was lower in Jerseys (26.9 %) and higher in Holsteins (30.7 %) and Holstein-Jersey crossbreeds (30.3 %) (P < 0.001). The molar concentrations of total VFA were higher at 3 DIM than at 93, 183, and 273 DIM, whereas the molar proportions of propionate were increased at 3 and 93 DIM, relative to 183 and 273 DIM. Rumen methanogen densities, distributions of the Mbr. species, and VFA molar proportions did not differ by breed. Conclusions: The data from the present study suggest that a core methanogen community is present among dairy breeds, through out a lactation. Furthermore, the methanogen communities were more influenced by DIM and the breed by DIM interactions than breed differences.en
dc.description.sponsorshipThe author's would like to acknowledge the USDA Hatch Grant (VT-H01801) and the UVM Dairy Center of Excellence as funding sources for animals and laboratory supplies. Furthermore, the Agriculture and Food Research Initiative Competitive Grant no. 2014-67016-21791 from the USDA National Institute of Food and Agriculture supported the VFA and feed analyses. We would like to thank Dr. Benoit St-Pierre of South Dakota State University for assistance with bioinformatics and Kurt Cotanch of the William H. Miner Institute for assistance with VFA analyses. We would also like to thank the Paul Miller Research Farm staff (Matt Boudette, Scott Shumway, Doug Watkin) for their on-farm maintenance and assistance. Lastly, we would like to thank Columbia University undergraduate student, Ryan C. Noyes, and the UVM undergraduate students (Pamela Bay, Katherine Boucher, Dylan Devino, Michael Eldredge, Samantha Frawley, Emma Hurley, Anne Kaufman, Danielle Semick, Mallory Sullivan, Sarah Zeger) who helped with calving, sampling, and milking.en
dc.language.isoenen
dc.publisherBIOMED CENTRAL LTDen
dc.relation.urlhttp://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-016-0694-7en
dc.rights© 2016 Cersosimo et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/).en
dc.subjectArchaeaen
dc.subjectDiversityen
dc.subjectHolstein-Jerseyen
dc.subjectJerseyen
dc.subject16S rRNA geneen
dc.subjectVolatile fatty acidsen
dc.titleInfluence of periparturient and postpartum diets on rumen methanogen communities in three breeds of primiparous dairy cowsen
dc.typeArticleen
dc.contributor.departmentUniv Arizona, Sch Anim & Comparat Biomed Scien
dc.identifier.journalBMC Microbiologyen
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en
dc.eprint.versionFinal published versionen
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