A Microfiltration Device for Urogenital Schistosomiasis Diagnostics

Persistent Link:
http://hdl.handle.net/10150/614655
Title:
A Microfiltration Device for Urogenital Schistosomiasis Diagnostics
Author:
Xiao, Yuan; Lu, Yi; Hsieh, Michael; Liao, Joseph; Wong, Pak Kin
Affiliation:
Univ Arizona, Dept Aerosp & Mech Engn
Issue Date:
2016-04-28
Publisher:
Public Library of Science
Citation:
A Microfiltration Device for Urogenital Schistosomiasis Diagnostics 2016, 11 (4):e0154640 PLOS ONE
Journal:
PLOS ONE
Rights:
© 2016 Xiao et al. This is an open access article distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Collection Information:
This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.
Abstract:
Schistosomiasis is a parasitic disease affecting over 200 million people worldwide. This study reports the design and development of a microfiltration device for isolating schistosome eggs in urine for rapid diagnostics of urogenital schistosomiasis. The design of the device comprises a linear array of microfluidic traps to immobilize and separate schistosome eggs. Sequential loading of individual eggs is achieved autonomously by flow resistance, which facilitates observation and enumeration of samples with low-abundance targets. Computational fluid dynamics modeling and experimental characterization are performed to optimize the trapping performance. By optimizing the capture strategy, the trapping efficiency could be achieved at 100% with 300 mu l/min and 83% with 3000 mu l/min, and the filtration procedure could be finished within 10 min. The trapped eggs can be either recovered for downstream analysis or preserved in situ for whole-mount staining. On-chip phenotyping using confocal laser fluorescence microscopy identifies the microstructure of the trapped schistosome eggs. The device provides a novel microfluidic approach for trapping, counting and on-chip fluorescence characterization of urinal Schistosoma haematobium eggs for clinical and investigative application.
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0154640
Keywords:
INFECTION; HAEMATOBIUM; MANSONI; BIOSENSORS
Version:
Final published version
Sponsors:
This work is supported by National Institutes of Health, Health Director's New Innovator Award (DP2OD007161).
Additional Links:
http://dx.plos.org/10.1371/journal.pone.0154640

Full metadata record

DC FieldValue Language
dc.contributor.authorXiao, Yuanen
dc.contributor.authorLu, Yien
dc.contributor.authorHsieh, Michaelen
dc.contributor.authorLiao, Josephen
dc.contributor.authorWong, Pak Kinen
dc.date.accessioned2016-06-24T20:57:41Z-
dc.date.available2016-06-24T20:57:41Z-
dc.date.issued2016-04-28-
dc.identifier.citationA Microfiltration Device for Urogenital Schistosomiasis Diagnostics 2016, 11 (4):e0154640 PLOS ONEen
dc.identifier.issn1932-6203-
dc.identifier.doi10.1371/journal.pone.0154640-
dc.identifier.urihttp://hdl.handle.net/10150/614655-
dc.description.abstractSchistosomiasis is a parasitic disease affecting over 200 million people worldwide. This study reports the design and development of a microfiltration device for isolating schistosome eggs in urine for rapid diagnostics of urogenital schistosomiasis. The design of the device comprises a linear array of microfluidic traps to immobilize and separate schistosome eggs. Sequential loading of individual eggs is achieved autonomously by flow resistance, which facilitates observation and enumeration of samples with low-abundance targets. Computational fluid dynamics modeling and experimental characterization are performed to optimize the trapping performance. By optimizing the capture strategy, the trapping efficiency could be achieved at 100% with 300 mu l/min and 83% with 3000 mu l/min, and the filtration procedure could be finished within 10 min. The trapped eggs can be either recovered for downstream analysis or preserved in situ for whole-mount staining. On-chip phenotyping using confocal laser fluorescence microscopy identifies the microstructure of the trapped schistosome eggs. The device provides a novel microfluidic approach for trapping, counting and on-chip fluorescence characterization of urinal Schistosoma haematobium eggs for clinical and investigative application.en
dc.description.sponsorshipThis work is supported by National Institutes of Health, Health Director's New Innovator Award (DP2OD007161).en
dc.language.isoenen
dc.publisherPublic Library of Scienceen
dc.relation.urlhttp://dx.plos.org/10.1371/journal.pone.0154640en
dc.rights© 2016 Xiao et al. This is an open access article distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are crediteden
dc.subjectINFECTIONen
dc.subjectHAEMATOBIUMen
dc.subjectMANSONIen
dc.subjectBIOSENSORSen
dc.titleA Microfiltration Device for Urogenital Schistosomiasis Diagnosticsen
dc.typeArticleen
dc.contributor.departmentUniv Arizona, Dept Aerosp & Mech Engnen
dc.identifier.journalPLOS ONEen
dc.description.collectioninformationThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu.en
dc.eprint.versionFinal published versionen
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