Optimization of Growth Conditions in Minimal Media for Production of Nucleolin Protein

Persistent Link:
http://hdl.handle.net/10150/613963
Title:
Optimization of Growth Conditions in Minimal Media for Production of Nucleolin Protein
Author:
Brewer, William Ryan; Yang, Danzhou; Bruce Carver, Megan
Affiliation:
College of Pharmacy, The University of Arizona
Issue Date:
2016
Rights:
Copyright © is held by the author.
Collection Information:
This item is part of the Pharmacy Student Research Projects collection, made available by the College of Pharmacy and the University Libraries at the University of Arizona. For more information about items in this collection, please contact Jennifer Martin, Associate Librarian and Clinical Instructor, Pharmacy Practice and Science, jenmartin@email.arizona.edu.
Publisher:
The University of Arizona.
Abstract:
Objectives: The purpose of this experiment is to compare NUC1234 expression levels from E. coli cultures by manipulating cell cultures according to the optical density (OD595) at which protein expression is induced, and the harvest time after induction. Methods: E. coli BL21(DE3) cells transformed with DNA to produce NUC1234 were plated and then grown in minimal media for protein expression. They were induced at a variety of optical densities and harvested at different times post-induction. Protein quantities from each sample were then compared using a protein determination assay and an SDS-PAGE gel. Results: The sample induced at an OD595 of 0.5 yielded a lower concentration of protein (0.874409mg/ml) compared to other samples; however, it visualized as a stronger band in the SDS-PAGE gel. The sample harvested at 6 hours yielded the largest protein concentration (1.347215mg/ml) among all active samples and appeared as bold as, or bolder than, those harvested at other times. Conclusions: The results of the protein assay study and gel visualization suggest that the optimal conditions for the production of NUC1234 are growth to an OD595 of 0.5 before induction of protein expression and a harvest time of 6 hours after induction.
Description:
Class of 2016 Abstract
Keywords:
Nucleolin Protein; NUC1234; Growth Conditions; E. coli
Advisor:
Yang, Danzhou; Bruce Carver, Megan

Full metadata record

DC FieldValue Language
dc.contributor.advisorYang, Danzhouen
dc.contributor.advisorBruce Carver, Meganen
dc.contributor.authorBrewer, William Ryanen
dc.contributor.authorYang, Danzhouen
dc.contributor.authorBruce Carver, Meganen
dc.date.accessioned2016-06-21T19:56:00Z-
dc.date.available2016-06-21T19:56:00Z-
dc.date.issued2016-
dc.identifier.urihttp://hdl.handle.net/10150/613963-
dc.descriptionClass of 2016 Abstracten
dc.description.abstractObjectives: The purpose of this experiment is to compare NUC1234 expression levels from E. coli cultures by manipulating cell cultures according to the optical density (OD595) at which protein expression is induced, and the harvest time after induction. Methods: E. coli BL21(DE3) cells transformed with DNA to produce NUC1234 were plated and then grown in minimal media for protein expression. They were induced at a variety of optical densities and harvested at different times post-induction. Protein quantities from each sample were then compared using a protein determination assay and an SDS-PAGE gel. Results: The sample induced at an OD595 of 0.5 yielded a lower concentration of protein (0.874409mg/ml) compared to other samples; however, it visualized as a stronger band in the SDS-PAGE gel. The sample harvested at 6 hours yielded the largest protein concentration (1.347215mg/ml) among all active samples and appeared as bold as, or bolder than, those harvested at other times. Conclusions: The results of the protein assay study and gel visualization suggest that the optimal conditions for the production of NUC1234 are growth to an OD595 of 0.5 before induction of protein expression and a harvest time of 6 hours after induction.en
dc.language.isoen_USen
dc.publisherThe University of Arizona.en
dc.rightsCopyright © is held by the author.en
dc.subjectNucleolin Proteinen
dc.subjectNUC1234en
dc.subjectGrowth Conditionsen
dc.subjectE. colien
dc.titleOptimization of Growth Conditions in Minimal Media for Production of Nucleolin Proteinen_US
dc.typetexten
dc.typeElectronic Reporten
dc.contributor.departmentCollege of Pharmacy, The University of Arizonaen
dc.description.collectioninformationThis item is part of the Pharmacy Student Research Projects collection, made available by the College of Pharmacy and the University Libraries at the University of Arizona. For more information about items in this collection, please contact Jennifer Martin, Associate Librarian and Clinical Instructor, Pharmacy Practice and Science, jenmartin@email.arizona.edu.en
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