Identifying Regulators of Lysosome Reformation: Inhibitor Screen in Mammalian Cell Culture

Persistent Link:
http://hdl.handle.net/10150/613352
Title:
Identifying Regulators of Lysosome Reformation: Inhibitor Screen in Mammalian Cell Culture
Author:
Liu, Ian
Issue Date:
2016
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Lysosomes are membrane-bound organelles that have diverse functions in eukaryotic cells. Malfunctions in lysosomes result in a range of diseases known as Lysosomal Storage Disorders. After fusing with late endosomes to form hybrid organelles, lysosomes bud off and are reformed in a poorly characterized process known as lysosome formation or reformation. Only one mammalian regulator of lysosome formation has been identified, the non-selective cation channel TRPML1. In the highly similar process of Autophagic Lysosome Reformation (ALR), three known regulators have also been identified, the vesicle-coating protein clathrin and two phosphatidylinositol kinases that catalyze the formation of the membrane phospholipid PI(4,5)P₂. Here, we use an inhibitor screen coupled with a live imaging assay to identify the actin microfilament as a novel regulator of lysosome formation.
Type:
text; Electronic Thesis
Keywords:
Lysosomal formation; Lysosome; Lysosome biogenesis; Molecular & Cellular Biology; Endocytosis
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College; Molecular & Cellular Biology
Degree Grantor:
University of Arizona
Advisor:
Fares, Hanna

Full metadata record

DC FieldValue Language
dc.language.isoen_USen
dc.titleIdentifying Regulators of Lysosome Reformation: Inhibitor Screen in Mammalian Cell Cultureen_US
dc.creatorLiu, Ianen
dc.contributor.authorLiu, Ianen
dc.date.issued2016-
dc.publisherThe University of Arizona.en
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en
dc.description.abstractLysosomes are membrane-bound organelles that have diverse functions in eukaryotic cells. Malfunctions in lysosomes result in a range of diseases known as Lysosomal Storage Disorders. After fusing with late endosomes to form hybrid organelles, lysosomes bud off and are reformed in a poorly characterized process known as lysosome formation or reformation. Only one mammalian regulator of lysosome formation has been identified, the non-selective cation channel TRPML1. In the highly similar process of Autophagic Lysosome Reformation (ALR), three known regulators have also been identified, the vesicle-coating protein clathrin and two phosphatidylinositol kinases that catalyze the formation of the membrane phospholipid PI(4,5)P₂. Here, we use an inhibitor screen coupled with a live imaging assay to identify the actin microfilament as a novel regulator of lysosome formation.en
dc.typetexten
dc.typeElectronic Thesisen
dc.subjectLysosomal formationen
dc.subjectLysosomeen
dc.subjectLysosome biogenesisen
dc.subjectMolecular & Cellular Biologyen
dc.subjectEndocytosisen
thesis.degree.nameM.S.en
thesis.degree.levelmastersen
thesis.degree.disciplineGraduate Collegeen
thesis.degree.disciplineMolecular & Cellular Biologyen
thesis.degree.grantorUniversity of Arizonaen
dc.contributor.advisorFares, Hannaen
dc.contributor.committeememberLaney, Jeffen
dc.contributor.committeememberBuchan, Rossen
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