QUANTITATIVE MEASUREMENT OF THE EXPRESSION OF TWO GENES IN THE CORETX AND CEREBELLUM OF A MOUSE MODEL OF JUVENILE ALZHEIMER’S

Persistent Link:
http://hdl.handle.net/10150/613283
Title:
QUANTITATIVE MEASUREMENT OF THE EXPRESSION OF TWO GENES IN THE CORETX AND CEREBELLUM OF A MOUSE MODEL OF JUVENILE ALZHEIMER’S
Author:
MARSHALL, CRAIG ANTHONY
Issue Date:
2016
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
There is a continuing effort in order to connect the two neurodegenerative diseases: Alzheimer’s Disease and Niemann-Pick Type C Disease (NPC1) “Juvenile Alzheimer’s”. Here in this study, we attempted to determine if there was a connection through the gene expression in inflammatory genes, CD14 and ETS-1, in a NPC1 mouse model, Npc1nmf164, using quantitative real time PCR. Hydroxypropyl-beta-cyclodextrin (HPβCD) is reported to provide a beneficial effect on NPC1, and there was interest in seeing if HPβCD treatment could return the expression levels to wildtype levels. Instead, only a significant decrease of the CD14 and ETS-1 in cerebellar tissue of untreated NPC1 affected mice (when compared to wildtype levels), and no significant difference for the treated, affected mice was seen. CD14 p value = 0.026, and ETS-1 p value = 0.014. There was no significant difference found in cortex tissue for either CD14 or ETS-1.
Type:
text; Electronic Thesis
Degree Name:
B.S.
Degree Level:
Bachelors
Degree Program:
Honors College; Biochemistry
Degree Grantor:
University of Arizona
Advisor:
Erickson, Robert P.

Full metadata record

DC FieldValue Language
dc.language.isoen_USen
dc.titleQUANTITATIVE MEASUREMENT OF THE EXPRESSION OF TWO GENES IN THE CORETX AND CEREBELLUM OF A MOUSE MODEL OF JUVENILE ALZHEIMER’Sen_US
dc.creatorMARSHALL, CRAIG ANTHONYen
dc.contributor.authorMARSHALL, CRAIG ANTHONYen
dc.date.issued2016-
dc.publisherThe University of Arizona.en
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en
dc.description.abstractThere is a continuing effort in order to connect the two neurodegenerative diseases: Alzheimer’s Disease and Niemann-Pick Type C Disease (NPC1) “Juvenile Alzheimer’s”. Here in this study, we attempted to determine if there was a connection through the gene expression in inflammatory genes, CD14 and ETS-1, in a NPC1 mouse model, Npc1nmf164, using quantitative real time PCR. Hydroxypropyl-beta-cyclodextrin (HPβCD) is reported to provide a beneficial effect on NPC1, and there was interest in seeing if HPβCD treatment could return the expression levels to wildtype levels. Instead, only a significant decrease of the CD14 and ETS-1 in cerebellar tissue of untreated NPC1 affected mice (when compared to wildtype levels), and no significant difference for the treated, affected mice was seen. CD14 p value = 0.026, and ETS-1 p value = 0.014. There was no significant difference found in cortex tissue for either CD14 or ETS-1.en
dc.typetexten
dc.typeElectronic Thesisen
thesis.degree.nameB.S.en
thesis.degree.levelBachelorsen
thesis.degree.disciplineHonors Collegeen
thesis.degree.disciplineBiochemistryen
thesis.degree.grantorUniversity of Arizonaen
dc.contributor.advisorErickson, Robert P.en
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