Persistent Link:
http://hdl.handle.net/10150/612977
Title:
IDENTIFYING PROTEINS INVOLVED IN LYSOSOME FORMATION
Author:
Gee, Kevin
Issue Date:
2016
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
There are many known proteins that are necessary for endocytosis at the plasma membrane. However, little is known about the proteins that are involved in lysosome formation. The Fares lab is interested in identifying novel proteins involved in lysosome formation. A forward genetic screen identified cup-12 with a phenotype that indicated a lysosome defect. Using complementation tests and sequencing, I identified cup-12 as clh-6. clh-6 is one of six ClC-type voltage-gated chloride channels in C. elegans. CLH-6 is most closely related to mammalian ClC6 and ClC7. Chloride channels regulate the excitability of cells, volume control, and organelle acidification. Mutations in chloride channels can lead to diseases such as Dent’s disease and Cystic Fibrosis. Our data suggest that loss of some ClCs causes lysosome dysfunction that could contribute to disease symptoms.
Type:
text; Electronic Thesis
Degree Name:
B.S.
Degree Level:
Bachelors
Degree Program:
Honors College; Neuroscience
Degree Grantor:
University of Arizona
Advisor:
Fares, Hanna (Johnny)

Full metadata record

DC FieldValue Language
dc.language.isoen_USen
dc.titleIDENTIFYING PROTEINS INVOLVED IN LYSOSOME FORMATIONen_US
dc.creatorGee, Kevinen
dc.contributor.authorGee, Kevinen
dc.date.issued2016-
dc.publisherThe University of Arizona.en
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en
dc.description.abstractThere are many known proteins that are necessary for endocytosis at the plasma membrane. However, little is known about the proteins that are involved in lysosome formation. The Fares lab is interested in identifying novel proteins involved in lysosome formation. A forward genetic screen identified cup-12 with a phenotype that indicated a lysosome defect. Using complementation tests and sequencing, I identified cup-12 as clh-6. clh-6 is one of six ClC-type voltage-gated chloride channels in C. elegans. CLH-6 is most closely related to mammalian ClC6 and ClC7. Chloride channels regulate the excitability of cells, volume control, and organelle acidification. Mutations in chloride channels can lead to diseases such as Dent’s disease and Cystic Fibrosis. Our data suggest that loss of some ClCs causes lysosome dysfunction that could contribute to disease symptoms.en
dc.typetexten
dc.typeElectronic Thesisen
thesis.degree.nameB.S.en
thesis.degree.levelBachelorsen
thesis.degree.disciplineHonors Collegeen
thesis.degree.disciplineNeuroscienceen
thesis.degree.grantorUniversity of Arizonaen
dc.contributor.advisorFares, Hanna (Johnny)en
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