SCLEROSTIN IMMUNOREACTIVITY INCREASES WITHIN THE CORTICAL BONE OSTEOCYTES IN THE FEMUR OF AGING MICE

Persistent Link:
http://hdl.handle.net/10150/612463
Title:
SCLEROSTIN IMMUNOREACTIVITY INCREASES WITHIN THE CORTICAL BONE OSTEOCYTES IN THE FEMUR OF AGING MICE
Author:
ABU HAMDAN, NATALIE
Issue Date:
2016
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Sclerostin, a secreted glycoprotein, is known to down-regulate osteocyte differentiation from osteoblasts and acts as a negative modulator of bone formation. It is well established that serum sclerostin levels increase with age but the relationship between changes of cellular expression of sclerostin with age is not well known. Immunohistochemical staining and confocal microscopic analysis of sclerostin immunoreactivity (sclerostin-IR) in the femurs of 4, 9, and 24 month old adult C3H/HeJ male mice was performed. Detectable levels of sclerostin-IR were found in cortical bone osteocytes of the femur in all age groups using an antibody directed against sclerostin. Phalloidin and DAPI were used to mark all osteocytes in cortical bone to detect osteocyte/ sclerostin-IR colocalization. Sclerostin-negative and sclerostin-positive expressing osteocytes were detected in close proximity throughout the cortical bone. Only a subset of osteocytes expressed sclerostin and this ratio of sclerostin positive osteocytes increased with age, from 38.5 ± 1.6% to 43 ± 3.6% to 49 ± 2.3% in young, middle-aged, and old mice, respectively. Understanding the potential mechanisms that drive these age-related changes may influence the therapeutic potential of age-related diseases like osteoporosis.
Type:
text; Electronic Thesis
Degree Name:
B.S.
Degree Level:
bachelors
Degree Program:
Honors College; Molecular and Cellular Biology
Degree Grantor:
University of Arizona
Advisor:
Mantyh, Patrick

Full metadata record

DC FieldValue Language
dc.language.isoen_USen
dc.titleSCLEROSTIN IMMUNOREACTIVITY INCREASES WITHIN THE CORTICAL BONE OSTEOCYTES IN THE FEMUR OF AGING MICEen_US
dc.creatorABU HAMDAN, NATALIEen
dc.contributor.authorABU HAMDAN, NATALIEen
dc.date.issued2016-
dc.publisherThe University of Arizona.en
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en
dc.description.abstractSclerostin, a secreted glycoprotein, is known to down-regulate osteocyte differentiation from osteoblasts and acts as a negative modulator of bone formation. It is well established that serum sclerostin levels increase with age but the relationship between changes of cellular expression of sclerostin with age is not well known. Immunohistochemical staining and confocal microscopic analysis of sclerostin immunoreactivity (sclerostin-IR) in the femurs of 4, 9, and 24 month old adult C3H/HeJ male mice was performed. Detectable levels of sclerostin-IR were found in cortical bone osteocytes of the femur in all age groups using an antibody directed against sclerostin. Phalloidin and DAPI were used to mark all osteocytes in cortical bone to detect osteocyte/ sclerostin-IR colocalization. Sclerostin-negative and sclerostin-positive expressing osteocytes were detected in close proximity throughout the cortical bone. Only a subset of osteocytes expressed sclerostin and this ratio of sclerostin positive osteocytes increased with age, from 38.5 ± 1.6% to 43 ± 3.6% to 49 ± 2.3% in young, middle-aged, and old mice, respectively. Understanding the potential mechanisms that drive these age-related changes may influence the therapeutic potential of age-related diseases like osteoporosis.en
dc.typetexten
dc.typeElectronic Thesisen
thesis.degree.nameB.S.en
thesis.degree.levelbachelorsen
thesis.degree.disciplineHonors Collegeen
thesis.degree.disciplineMolecular and Cellular Biologyen
thesis.degree.grantorUniversity of Arizonaen
dc.contributor.advisorMantyh, Patricken
All Items in UA Campus Repository are protected by copyright, with all rights reserved, unless otherwise indicated.