Genomic resources for Myzus persicae: EST sequencing, SNP identification, and microarray design

Persistent Link:
http://hdl.handle.net/10150/610401
Title:
Genomic resources for Myzus persicae: EST sequencing, SNP identification, and microarray design
Author:
Ramsey, John; Wilson, Alex; de Vos, Martin; Sun, Qi; Tamborindeguy, Cecilia; Winfield, Agnese; Malloch, Gaynor; Smith, Dawn; Fenton, Brian; Gray, Stewart; Jander, Georg
Affiliation:
Boyce Thompson Institute for Plant Research, Tower Road, Ithaca, NY 14853, USA; Department of Biology, University of Miami, Coral Gables, Florida 33146, USA; Department of Ecology and Evolutionary Biology, University of Arizona, Tucson, Arizona 85721, USA; Cornell Theory Center, Cornell University, Ithaca, NY 14853, USA; USDA/ARS, Plant Protection Research Unit, Ithaca, NY 14853, USA; Scottish Crop Research Institute, Invergowrie, Dundee, UK
Issue Date:
2007
Publisher:
BioMed Central
Citation:
BMC Genomics 2007, 8:423 doi:10.1186/1471-2164-8-423
Journal:
BMC Genomics
Rights:
© 2007 Ramsey et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0)
Collection Information:
This item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.
Abstract:
BACKGROUND:The green peach aphid, Myzus persicae (Sulzer), is a world-wide insect pest capable of infesting more than 40 plant families, including many crop species. However, despite the significant damage inflicted by M. persicae in agricultural systems through direct feeding damage and by its ability to transmit plant viruses, limited genomic information is available for this species.RESULTS:Sequencing of 16 M. persicae cDNA libraries generated 26,669 expressed sequence tags (ESTs). Aphids for library construction were raised on Arabidopsis thaliana, Nicotiana benthamiana, Brassica oleracea, B. napus, and Physalis floridana (with and without Potato leafroll virus infection). The M. persicae cDNA libraries include ones made from sexual and asexual whole aphids, guts, heads, and salivary glands. In silico comparison of cDNA libraries identified aphid genes with tissue-specific expression patterns, and gene expression that is induced by feeding on Nicotiana benthamiana. Furthermore, 2423 genes that are novel to science and potentially aphid-specific were identified. Comparison of cDNA data from three aphid lineages identified single nucleotide polymorphisms that can be used as genetic markers and, in some cases, may represent functional differences in the protein products. In particular, non-conservative amino acid substitutions in a highly expressed gut protease may be of adaptive significance for M. persicae feeding on different host plants. The Agilent eArray platform was used to design an M. persicae oligonucleotide microarray representing over 10,000 unique genes.CONCLUSION:New genomic resources have been developed for M. persicae, an agriculturally important insect pest. These include previously unknown sequence data, a collection of expressed genes, molecular markers, and a DNA microarray that can be used to study aphid gene expression. These resources will help elucidate the adaptations that allow M. persicae to develop compatible interactions with its host plants, complementing ongoing work illuminating plant molecular responses to phloem-feeding insects.
EISSN:
1471-2164
DOI:
10.1186/1471-2164-8-423
Version:
Final published version
Additional Links:
http://www.biomedcentral.com/1471-2164/8/423

Full metadata record

DC FieldValue Language
dc.contributor.authorRamsey, Johnen
dc.contributor.authorWilson, Alexen
dc.contributor.authorde Vos, Martinen
dc.contributor.authorSun, Qien
dc.contributor.authorTamborindeguy, Ceciliaen
dc.contributor.authorWinfield, Agneseen
dc.contributor.authorMalloch, Gaynoren
dc.contributor.authorSmith, Dawnen
dc.contributor.authorFenton, Brianen
dc.contributor.authorGray, Stewarten
dc.contributor.authorJander, Georgen
dc.date.accessioned2016-05-20T09:06:00Z-
dc.date.available2016-05-20T09:06:00Z-
dc.date.issued2007en
dc.identifier.citationBMC Genomics 2007, 8:423 doi:10.1186/1471-2164-8-423en
dc.identifier.doi10.1186/1471-2164-8-423en
dc.identifier.urihttp://hdl.handle.net/10150/610401-
dc.description.abstractBACKGROUND:The green peach aphid, Myzus persicae (Sulzer), is a world-wide insect pest capable of infesting more than 40 plant families, including many crop species. However, despite the significant damage inflicted by M. persicae in agricultural systems through direct feeding damage and by its ability to transmit plant viruses, limited genomic information is available for this species.RESULTS:Sequencing of 16 M. persicae cDNA libraries generated 26,669 expressed sequence tags (ESTs). Aphids for library construction were raised on Arabidopsis thaliana, Nicotiana benthamiana, Brassica oleracea, B. napus, and Physalis floridana (with and without Potato leafroll virus infection). The M. persicae cDNA libraries include ones made from sexual and asexual whole aphids, guts, heads, and salivary glands. In silico comparison of cDNA libraries identified aphid genes with tissue-specific expression patterns, and gene expression that is induced by feeding on Nicotiana benthamiana. Furthermore, 2423 genes that are novel to science and potentially aphid-specific were identified. Comparison of cDNA data from three aphid lineages identified single nucleotide polymorphisms that can be used as genetic markers and, in some cases, may represent functional differences in the protein products. In particular, non-conservative amino acid substitutions in a highly expressed gut protease may be of adaptive significance for M. persicae feeding on different host plants. The Agilent eArray platform was used to design an M. persicae oligonucleotide microarray representing over 10,000 unique genes.CONCLUSION:New genomic resources have been developed for M. persicae, an agriculturally important insect pest. These include previously unknown sequence data, a collection of expressed genes, molecular markers, and a DNA microarray that can be used to study aphid gene expression. These resources will help elucidate the adaptations that allow M. persicae to develop compatible interactions with its host plants, complementing ongoing work illuminating plant molecular responses to phloem-feeding insects.en
dc.language.isoenen
dc.publisherBioMed Centralen
dc.relation.urlhttp://www.biomedcentral.com/1471-2164/8/423en
dc.rights© 2007 Ramsey et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0)en
dc.titleGenomic resources for Myzus persicae: EST sequencing, SNP identification, and microarray designen
dc.typeArticleen
dc.identifier.eissn1471-2164en
dc.contributor.departmentBoyce Thompson Institute for Plant Research, Tower Road, Ithaca, NY 14853, USAen
dc.contributor.departmentDepartment of Biology, University of Miami, Coral Gables, Florida 33146, USAen
dc.contributor.departmentDepartment of Ecology and Evolutionary Biology, University of Arizona, Tucson, Arizona 85721, USAen
dc.contributor.departmentCornell Theory Center, Cornell University, Ithaca, NY 14853, USAen
dc.contributor.departmentUSDA/ARS, Plant Protection Research Unit, Ithaca, NY 14853, USAen
dc.contributor.departmentScottish Crop Research Institute, Invergowrie, Dundee, UKen
dc.identifier.journalBMC Genomicsen
dc.description.collectioninformationThis item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.en
dc.eprint.versionFinal published versionen
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