SPE-8, a protein-tyrosine kinase, localizes to the spermatid cell membrane through interaction with other members of the SPE-8 group spermatid activation signaling pathway in C. elegans

Persistent Link:
http://hdl.handle.net/10150/610393
Title:
SPE-8, a protein-tyrosine kinase, localizes to the spermatid cell membrane through interaction with other members of the SPE-8 group spermatid activation signaling pathway in C. elegans
Author:
Muhlrad, Paul; Clark, Jessica; Nasri, Ubaydah; Sullivan, Nicholas; LaMunyon, Craig
Affiliation:
Department of Molecular and Cellular Biology, University of Arizona, 1007 Lowell St., Tucson, AZ 85721, USA; Department of Molecular, Cellular and Developmental Biology, Currently, University of Colorado Boulder, 347 UCB, Boulder, CO 80309, USA; Department of Biological Science, California State Polytechnic University, 3801 W. Temple Ave, Pomona, CA 91768, USA
Issue Date:
2014
Publisher:
BioMed Central
Citation:
Muhlrad et al. BMC Genetics 2014, 15:83 http://www.biomedcentral.com/1471-2156/15/83
Journal:
BMC Genetics
Rights:
© 2014 Muhlrad et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0)
Collection Information:
This item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.
Abstract:
BACKGROUND:The SPE-8 group gene products transduce the signal for spermatid activation initiated by extracellular zinc in C. elegans. Mutations in the spe-8 group genes result in hermaphrodite-derived spermatids that cannot activate to crawling spermatozoa, although spermatids from mutant males activate through a pathway induced by extracellular TRY-5 protease present in male seminal fluid.RESULTS:Here, we identify SPE-8 as a member of a large family of sperm-expressed non-receptor-like protein-tyrosine kinases. A rescuing SPE-8::GFP translational fusion reporter localizes to the plasma membrane in all spermatogenic cells from the primary spermatocyte stage through spermatids. Once spermatids become activated to spermatozoa, the reporter moves from the plasma membrane to the cytoplasm. Mutations in the spe-8 group genes spe-12, spe-19, and spe-27 disrupt localization of the reporter to the plasma membrane, while localization appears near normal in a spe-29 mutant background.CONCLUSIONS:These results suggest that the SPE-8 group proteins form a functional complex localized at the plasma membrane, and that SPE-8 is correctly positioned only when all members of the SPE-8 group are present, with the possible exception of SPE-29. Further, SPE-8 is released from the membrane when the activation signal is transduced into the spermatid.
EISSN:
1471-2156
DOI:
10.1186/1471-2156-15-83
Keywords:
Caenorhabditis elegans; Spermatogenesis; Sperm activation; spe-8; Signal transduction
Version:
Final published version
Additional Links:
http://www.biomedcentral.com/1471-2156/15/83

Full metadata record

DC FieldValue Language
dc.contributor.authorMuhlrad, Paulen
dc.contributor.authorClark, Jessicaen
dc.contributor.authorNasri, Ubaydahen
dc.contributor.authorSullivan, Nicholasen
dc.contributor.authorLaMunyon, Craigen
dc.date.accessioned2016-05-20T09:05:49Z-
dc.date.available2016-05-20T09:05:49Z-
dc.date.issued2014en
dc.identifier.citationMuhlrad et al. BMC Genetics 2014, 15:83 http://www.biomedcentral.com/1471-2156/15/83en
dc.identifier.doi10.1186/1471-2156-15-83en
dc.identifier.urihttp://hdl.handle.net/10150/610393-
dc.description.abstractBACKGROUND:The SPE-8 group gene products transduce the signal for spermatid activation initiated by extracellular zinc in C. elegans. Mutations in the spe-8 group genes result in hermaphrodite-derived spermatids that cannot activate to crawling spermatozoa, although spermatids from mutant males activate through a pathway induced by extracellular TRY-5 protease present in male seminal fluid.RESULTS:Here, we identify SPE-8 as a member of a large family of sperm-expressed non-receptor-like protein-tyrosine kinases. A rescuing SPE-8::GFP translational fusion reporter localizes to the plasma membrane in all spermatogenic cells from the primary spermatocyte stage through spermatids. Once spermatids become activated to spermatozoa, the reporter moves from the plasma membrane to the cytoplasm. Mutations in the spe-8 group genes spe-12, spe-19, and spe-27 disrupt localization of the reporter to the plasma membrane, while localization appears near normal in a spe-29 mutant background.CONCLUSIONS:These results suggest that the SPE-8 group proteins form a functional complex localized at the plasma membrane, and that SPE-8 is correctly positioned only when all members of the SPE-8 group are present, with the possible exception of SPE-29. Further, SPE-8 is released from the membrane when the activation signal is transduced into the spermatid.en
dc.language.isoenen
dc.publisherBioMed Centralen
dc.relation.urlhttp://www.biomedcentral.com/1471-2156/15/83en
dc.rights© 2014 Muhlrad et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0)en
dc.subjectCaenorhabditis elegansen
dc.subjectSpermatogenesisen
dc.subjectSperm activationen
dc.subjectspe-8en
dc.subjectSignal transductionen
dc.titleSPE-8, a protein-tyrosine kinase, localizes to the spermatid cell membrane through interaction with other members of the SPE-8 group spermatid activation signaling pathway in C. elegansen
dc.typeArticleen
dc.identifier.eissn1471-2156en
dc.contributor.departmentDepartment of Molecular and Cellular Biology, University of Arizona, 1007 Lowell St., Tucson, AZ 85721, USAen
dc.contributor.departmentDepartment of Molecular, Cellular and Developmental Biology, Currently, University of Colorado Boulder, 347 UCB, Boulder, CO 80309, USAen
dc.contributor.departmentDepartment of Biological Science, California State Polytechnic University, 3801 W. Temple Ave, Pomona, CA 91768, USAen
dc.identifier.journalBMC Geneticsen
dc.description.collectioninformationThis item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.en
dc.eprint.versionFinal published versionen
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