Inferring angiosperm phylogeny from EST data with widespread gene duplication

Persistent Link:
http://hdl.handle.net/10150/610375
Title:
Inferring angiosperm phylogeny from EST data with widespread gene duplication
Author:
Sanderson, Michael; McMahon, Michelle
Affiliation:
Department of Ecology and Evolutionary Biology, University of Arizona, Tucson, AZ 85721, USA; Department of Plant Sciences, University of Arizona, Tucson, AZ 85721, USA
Issue Date:
2007
Publisher:
BioMed Central
Citation:
BMC Evolutionary Biology 2007, 7(Suppl 1):S3 doi:10.1186/1471-2148-7-S1-S3
Journal:
BMC Evolutionary Biology
Rights:
© 2007 Sanderson and McMahon; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0)
Collection Information:
This item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.
Abstract:
BACKGROUND:Most studies inferring species phylogenies use sequences from single copy genes or sets of orthologs culled from gene families. For taxa such as plants, with very high levels of gene duplication in their nuclear genomes, this has limited the exploitation of nuclear sequences for phylogenetic studies, such as those available in large EST libraries. One rarely used method of inference, gene tree parsimony, can infer species trees from gene families undergoing duplication and loss, but its performance has not been evaluated at a phylogenomic scale for EST data in plants.RESULTS:A gene tree parsimony analysis based on EST data was undertaken for six angiosperm model species and Pinus, an outgroup. Although a large fraction of the tentative consensus sequences obtained from the TIGR database of ESTs was assembled into homologous clusters too small to be phylogenetically informative, some 557 clusters contained promising levels of information. Based on maximum likelihood estimates of the gene trees obtained from these clusters, gene tree parsimony correctly inferred the accepted species tree with strong statistical support. A slight variant of this species tree was obtained when maximum parsimony was used to infer the individual gene trees instead.CONCLUSION:Despite the complexity of the EST data and the relatively small fraction eventually used in inferring a species tree, the gene tree parsimony method performed well in the face of very high apparent rates of duplication.
EISSN:
1471-2148
DOI:
10.1186/1471-2148-7-S1-S3
Version:
Final published version
Additional Links:
http://www.biomedcentral.com/1471-2148/7/S1/S3

Full metadata record

DC FieldValue Language
dc.contributor.authorSanderson, Michaelen
dc.contributor.authorMcMahon, Michelleen
dc.date.accessioned2016-05-20T09:05:24Z-
dc.date.available2016-05-20T09:05:24Z-
dc.date.issued2007en
dc.identifier.citationBMC Evolutionary Biology 2007, 7(Suppl 1):S3 doi:10.1186/1471-2148-7-S1-S3en
dc.identifier.doi10.1186/1471-2148-7-S1-S3en
dc.identifier.urihttp://hdl.handle.net/10150/610375-
dc.description.abstractBACKGROUND:Most studies inferring species phylogenies use sequences from single copy genes or sets of orthologs culled from gene families. For taxa such as plants, with very high levels of gene duplication in their nuclear genomes, this has limited the exploitation of nuclear sequences for phylogenetic studies, such as those available in large EST libraries. One rarely used method of inference, gene tree parsimony, can infer species trees from gene families undergoing duplication and loss, but its performance has not been evaluated at a phylogenomic scale for EST data in plants.RESULTS:A gene tree parsimony analysis based on EST data was undertaken for six angiosperm model species and Pinus, an outgroup. Although a large fraction of the tentative consensus sequences obtained from the TIGR database of ESTs was assembled into homologous clusters too small to be phylogenetically informative, some 557 clusters contained promising levels of information. Based on maximum likelihood estimates of the gene trees obtained from these clusters, gene tree parsimony correctly inferred the accepted species tree with strong statistical support. A slight variant of this species tree was obtained when maximum parsimony was used to infer the individual gene trees instead.CONCLUSION:Despite the complexity of the EST data and the relatively small fraction eventually used in inferring a species tree, the gene tree parsimony method performed well in the face of very high apparent rates of duplication.en
dc.language.isoenen
dc.publisherBioMed Centralen
dc.relation.urlhttp://www.biomedcentral.com/1471-2148/7/S1/S3en
dc.rights© 2007 Sanderson and McMahon; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0)en
dc.titleInferring angiosperm phylogeny from EST data with widespread gene duplicationen
dc.typeArticleen
dc.identifier.eissn1471-2148en
dc.contributor.departmentDepartment of Ecology and Evolutionary Biology, University of Arizona, Tucson, AZ 85721, USAen
dc.contributor.departmentDepartment of Plant Sciences, University of Arizona, Tucson, AZ 85721, USAen
dc.identifier.journalBMC Evolutionary Biologyen
dc.description.collectioninformationThis item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.en
dc.eprint.versionFinal published versionen
All Items in UA Campus Repository are protected by copyright, with all rights reserved, unless otherwise indicated.