Lysosomal trafficking functions of mucolipin-1 in murine macrophages

Persistent Link:
http://hdl.handle.net/10150/610354
Title:
Lysosomal trafficking functions of mucolipin-1 in murine macrophages
Author:
Thompson, Eric; Schaheen, Lara; Dang, Hope; Fares, Hanna
Affiliation:
Department of Molecular and Cellular Biology, Life Sciences South Room 531, University of Arizona, Tucson, AZ 85721, USA
Issue Date:
2007
Publisher:
BioMed Central
Citation:
BMC Cell Biology 2007, 8:54 doi:10.1186/1471-2121-8-54
Journal:
BMC Cell Biology
Rights:
© 2007 Thompson et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0)
Collection Information:
This item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.
Abstract:
BACKGROUND:Mucolipidosis Type IV is currently characterized as a lysosomal storage disorder with defects that include corneal clouding, achlorhydria and psychomotor retardation. MCOLN1, the gene responsible for this disease, encodes the protein mucolipin-1 that belongs to the "Transient Receptor Potential" family of proteins and has been shown to function as a non-selective cation channel whose activity is modulated by pH. Two cell biological defects that have been described in MLIV fibroblasts are a hyperacidification of lysosomes and a delay in the exit of lipids from lysosomes.RESULTS:We show that mucolipin-1 localizes to lysosomal compartments in RAW264.7 mouse macrophages that show subcompartmental accumulations of endocytosed molecules. Using stable RNAi clones, we show that mucolipin-1 is required for the exit of lipids from these compartments, for the transport of endocytosed molecules to terminal lysosomes, and for the transport of the Major Histocompatibility Complex II to the plasma membrane.CONCLUSION:Mucolipin-1 functions in the efficient exit of molecules, destined for various cellular organelles, from lysosomal compartments.
EISSN:
1471-2121
DOI:
10.1186/1471-2121-8-54
Version:
Final published version
Additional Links:
http://www.biomedcentral.com/1471-2121/8/54

Full metadata record

DC FieldValue Language
dc.contributor.authorThompson, Ericen
dc.contributor.authorSchaheen, Laraen
dc.contributor.authorDang, Hopeen
dc.contributor.authorFares, Hannaen
dc.date.accessioned2016-05-20T09:04:57Z-
dc.date.available2016-05-20T09:04:57Z-
dc.date.issued2007en
dc.identifier.citationBMC Cell Biology 2007, 8:54 doi:10.1186/1471-2121-8-54en
dc.identifier.doi10.1186/1471-2121-8-54en
dc.identifier.urihttp://hdl.handle.net/10150/610354-
dc.description.abstractBACKGROUND:Mucolipidosis Type IV is currently characterized as a lysosomal storage disorder with defects that include corneal clouding, achlorhydria and psychomotor retardation. MCOLN1, the gene responsible for this disease, encodes the protein mucolipin-1 that belongs to the "Transient Receptor Potential" family of proteins and has been shown to function as a non-selective cation channel whose activity is modulated by pH. Two cell biological defects that have been described in MLIV fibroblasts are a hyperacidification of lysosomes and a delay in the exit of lipids from lysosomes.RESULTS:We show that mucolipin-1 localizes to lysosomal compartments in RAW264.7 mouse macrophages that show subcompartmental accumulations of endocytosed molecules. Using stable RNAi clones, we show that mucolipin-1 is required for the exit of lipids from these compartments, for the transport of endocytosed molecules to terminal lysosomes, and for the transport of the Major Histocompatibility Complex II to the plasma membrane.CONCLUSION:Mucolipin-1 functions in the efficient exit of molecules, destined for various cellular organelles, from lysosomal compartments.en
dc.language.isoenen
dc.publisherBioMed Centralen
dc.relation.urlhttp://www.biomedcentral.com/1471-2121/8/54en
dc.rights© 2007 Thompson et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0)en
dc.titleLysosomal trafficking functions of mucolipin-1 in murine macrophagesen
dc.typeArticleen
dc.identifier.eissn1471-2121en
dc.contributor.departmentDepartment of Molecular and Cellular Biology, Life Sciences South Room 531, University of Arizona, Tucson, AZ 85721, USAen
dc.identifier.journalBMC Cell Biologyen
dc.description.collectioninformationThis item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.en
dc.eprint.versionFinal published versionen
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