The anti-tumor efficacy of 2-deoxyglucose and D-allose are enhanced with p38 inhibition in pancreatic and ovarian cell lines

Persistent Link:
http://hdl.handle.net/10150/610319
Title:
The anti-tumor efficacy of 2-deoxyglucose and D-allose are enhanced with p38 inhibition in pancreatic and ovarian cell lines
Author:
Malm, S. W.; Hanke, N. T.; Gill, A.; Carbajal, L.; Baker, A. F.
Affiliation:
College of Pharmacy, University of Arizona; College of Medicine, University of Arizona Cancer Center
Issue Date:
2015
Publisher:
BioMed Central
Citation:
Malm et al. Journal of Experimental & Clinical Cancer Research (2015) 34:31 DOI 10.1186/s13046-015-0147-4
Journal:
Journal of Experimental & Clinical Cancer Research
Rights:
© 2015 Malm et al.; licensee BioMed Central. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0)
Collection Information:
This item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.
Abstract:
PURPOSE: The anti-tumor activity of glucose analogs 2-deoxy-glucose (2-DG) and D-allose was investigated alone or in combination with p38 mitogen-activated protein kinase (MAPK) inhibitor SB202190 or platinum analogs as a strategy to pharmacologically target glycolytic tumor phenotypes. METHODS: Hypoxia inducible factor-1 alpha (HIF-1alpha) protein accumulation in pancreatic cell lines treated with SB202190 alone and in combination with glucose analogs was analyzed by Western blot. HIF-1alpha transcriptional activity was measured in MIA PaCa-2 cells stably transfected with a hypoxia response element luciferase reporter following treatment with glucose analogs alone, and in combination with SB202190. Induction of cleaved poly(ADP-ribose) polymerase (PARP) was measured by Western blot in the MIA PaCa-2 cells. In vitro anti-proliferative activity of 2-DG and D-allose alone, or in combination with oxaliplatin (pancreatic cell lines), cisplatin (ovarian cell lines), or with SB202190 were investigated using the MTT assay. RESULTS: SB202190 decreased HIF-1alpha protein accumulation and transcriptional activity. 2-DG demonstrated greater anti-proliferative activity than D-allose. Pre-treatment with SB202190 enhanced activity of both 2-DG and D-allose in MIA PaCa-2, BxPC-3, ASPC-1, and SK-OV-3 cells. The combination of D-allose and platinum agents was additive to moderately synergistic in all but the OVCAR-3 and HEY cells. SB202190 pre-treatment further enhanced activity of D-allose and 2-DG with platinum agents in most cell lines investigated. CONCLUSIONS: SB202190 induced sensitization of tumor cells to 2-DG and D-allose may be partially mediated by inhibition of HIF-1alpha activity. Combining glucose analogs and p38 MAPK inhibitors with chemotherapy may be an effective approach to target glycolytic tumor phenotypes.
EISSN:
1756-9966
PubMed ID:
25888489
PubMed Central ID:
PMC4391305
DOI:
10.1186/s13046-015-0147-4 [doi]
Keywords:
2-deoxy-glucose; D-allose; Hypoxia inducible factor 1α; Pancreatic cancer; Ovarian cancer; p38 mitogen activated protein kinase
Version:
Final published version
Additional Links:
http://jeccr.biomedcentral.com/articles/10.1186/s13046-015-0147-4

Full metadata record

DC FieldValue Language
dc.contributor.authorMalm, S. W.en
dc.contributor.authorHanke, N. T.en
dc.contributor.authorGill, A.en
dc.contributor.authorCarbajal, L.en
dc.contributor.authorBaker, A. F.en
dc.date.accessioned2016-05-20T09:04:06Z-
dc.date.available2016-05-20T09:04:06Z-
dc.date.issued2015en
dc.identifier.citationMalm et al. Journal of Experimental & Clinical Cancer Research (2015) 34:31 DOI 10.1186/s13046-015-0147-4en
dc.identifier.pmid25888489en
dc.identifier.doi10.1186/s13046-015-0147-4 [doi]en
dc.identifier.urihttp://hdl.handle.net/10150/610319-
dc.description.abstractPURPOSE: The anti-tumor activity of glucose analogs 2-deoxy-glucose (2-DG) and D-allose was investigated alone or in combination with p38 mitogen-activated protein kinase (MAPK) inhibitor SB202190 or platinum analogs as a strategy to pharmacologically target glycolytic tumor phenotypes. METHODS: Hypoxia inducible factor-1 alpha (HIF-1alpha) protein accumulation in pancreatic cell lines treated with SB202190 alone and in combination with glucose analogs was analyzed by Western blot. HIF-1alpha transcriptional activity was measured in MIA PaCa-2 cells stably transfected with a hypoxia response element luciferase reporter following treatment with glucose analogs alone, and in combination with SB202190. Induction of cleaved poly(ADP-ribose) polymerase (PARP) was measured by Western blot in the MIA PaCa-2 cells. In vitro anti-proliferative activity of 2-DG and D-allose alone, or in combination with oxaliplatin (pancreatic cell lines), cisplatin (ovarian cell lines), or with SB202190 were investigated using the MTT assay. RESULTS: SB202190 decreased HIF-1alpha protein accumulation and transcriptional activity. 2-DG demonstrated greater anti-proliferative activity than D-allose. Pre-treatment with SB202190 enhanced activity of both 2-DG and D-allose in MIA PaCa-2, BxPC-3, ASPC-1, and SK-OV-3 cells. The combination of D-allose and platinum agents was additive to moderately synergistic in all but the OVCAR-3 and HEY cells. SB202190 pre-treatment further enhanced activity of D-allose and 2-DG with platinum agents in most cell lines investigated. CONCLUSIONS: SB202190 induced sensitization of tumor cells to 2-DG and D-allose may be partially mediated by inhibition of HIF-1alpha activity. Combining glucose analogs and p38 MAPK inhibitors with chemotherapy may be an effective approach to target glycolytic tumor phenotypes.en
dc.language.isoenen
dc.publisherBioMed Centralen
dc.relation.urlhttp://jeccr.biomedcentral.com/articles/10.1186/s13046-015-0147-4en
dc.rights© 2015 Malm et al.; licensee BioMed Central. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0)en
dc.subject2-deoxy-glucoseen
dc.subjectD-alloseen
dc.subjectHypoxia inducible factor 1αen
dc.subjectPancreatic canceren
dc.subjectOvarian canceren
dc.subjectp38 mitogen activated protein kinaseen
dc.titleThe anti-tumor efficacy of 2-deoxyglucose and D-allose are enhanced with p38 inhibition in pancreatic and ovarian cell linesen
dc.typeArticleen
dc.identifier.eissn1756-9966en
dc.contributor.departmentCollege of Pharmacy, University of Arizonaen
dc.contributor.departmentCollege of Medicine, University of Arizona Cancer Centeren
dc.identifier.journalJournal of Experimental & Clinical Cancer Researchen
dc.identifier.pmcidPMC4391305en
dc.description.collectioninformationThis item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.en
dc.eprint.versionFinal published versionen
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