Experimental study on cryotherapy for fungal corneal ulcer

Persistent Link:
http://hdl.handle.net/10150/610295
Title:
Experimental study on cryotherapy for fungal corneal ulcer
Author:
Chen, Yingxin; Yang, Weijia; Gao, Minghong; Belin, Michael Wellington; Yu, Hai; Yu, Jing
Affiliation:
Department of Ophthalmology, General Hospital of Shenyang Military Area Command; Dalian Medical University; Department of Ophthalmology, University of Arizona, Arizona Health Sciences Center
Issue Date:
2015
Publisher:
Springer
Citation:
Chen et al. BMC Ophthalmology (2015) 15:29 DOI 10.1186/s12886-015-0011-5
Journal:
BMC Ophthalmology
Rights:
© 2015 Chen et al.; licensee BioMed Central. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0)
Collection Information:
This item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.
Abstract:
BACKGROUND: Fungal corneal ulcer is one of the major causes of visual impairment worldwide. Treatment of fungal corneal ulcer mainly depends on anti-fungal agents. In the current study, we developed an integrated combination therapy of cryotherapy and anti-fungal agents to facilitate effective treatment of fungal corneal ulcer. METHODS: Rabbit models of cornea infection were established using a combined method of intrastromal injection and keratoplasty. After treatment with cryotherapy and anti-fungal agents, scanning electron microscopy, transmission electron microscopy, and confocal microscopy were conducted to observe changes in microstructure in the rabbits. Periodic acid Schiff A and hematoxylin and eosin staining were used for detection of histological changes. RESULTS: Continuous scanning electron microscopy and transmission electron microscopy observations showed that cryothermal treatment inhibited growth of fungal mycelium by destroying fungal cellular structures. Typical cryotherapy was effective in curing fungal corneal ulcer. Different fungi showed different susceptibilities to treatment. The curative effect of Candida albicans was the best, while that of Aspergillus fumigates was the worst. CONCLUSIONS: Our study provides a novel method of a combination of cryotherapy and anti-fungal agents for treatment of fungal corneal ulcer. This treatment could help facilitate the practice of fungal keratitis treatment in the future.
EISSN:
1471-2415
DOI:
10.1186/s12886-015-0011-5
Keywords:
Fungal corneal keratitis; Cryotherapy; Anti-fungal agents
Version:
Final published version
Additional Links:
http://www.biomedcentral.com/1471-2415/15/29

Full metadata record

DC FieldValue Language
dc.contributor.authorChen, Yingxinen
dc.contributor.authorYang, Weijiaen
dc.contributor.authorGao, Minghongen
dc.contributor.authorBelin, Michael Wellingtonen
dc.contributor.authorYu, Haien
dc.contributor.authorYu, Jingen
dc.date.accessioned2016-05-20T09:03:30Z-
dc.date.available2016-05-20T09:03:30Z-
dc.date.issued2015en
dc.identifier.citationChen et al. BMC Ophthalmology (2015) 15:29 DOI 10.1186/s12886-015-0011-5en
dc.identifier.doi10.1186/s12886-015-0011-5en
dc.identifier.urihttp://hdl.handle.net/10150/610295-
dc.description.abstractBACKGROUND: Fungal corneal ulcer is one of the major causes of visual impairment worldwide. Treatment of fungal corneal ulcer mainly depends on anti-fungal agents. In the current study, we developed an integrated combination therapy of cryotherapy and anti-fungal agents to facilitate effective treatment of fungal corneal ulcer. METHODS: Rabbit models of cornea infection were established using a combined method of intrastromal injection and keratoplasty. After treatment with cryotherapy and anti-fungal agents, scanning electron microscopy, transmission electron microscopy, and confocal microscopy were conducted to observe changes in microstructure in the rabbits. Periodic acid Schiff A and hematoxylin and eosin staining were used for detection of histological changes. RESULTS: Continuous scanning electron microscopy and transmission electron microscopy observations showed that cryothermal treatment inhibited growth of fungal mycelium by destroying fungal cellular structures. Typical cryotherapy was effective in curing fungal corneal ulcer. Different fungi showed different susceptibilities to treatment. The curative effect of Candida albicans was the best, while that of Aspergillus fumigates was the worst. CONCLUSIONS: Our study provides a novel method of a combination of cryotherapy and anti-fungal agents for treatment of fungal corneal ulcer. This treatment could help facilitate the practice of fungal keratitis treatment in the future.en
dc.language.isoenen
dc.publisherSpringeren
dc.relation.urlhttp://www.biomedcentral.com/1471-2415/15/29en
dc.rights© 2015 Chen et al.; licensee BioMed Central. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0)en
dc.subjectFungal corneal keratitisen
dc.subjectCryotherapyen
dc.subjectAnti-fungal agentsen
dc.titleExperimental study on cryotherapy for fungal corneal ulceren
dc.typeArticleen
dc.identifier.eissn1471-2415en
dc.contributor.departmentDepartment of Ophthalmology, General Hospital of Shenyang Military Area Commanden
dc.contributor.departmentDalian Medical Universityen
dc.contributor.departmentDepartment of Ophthalmology, University of Arizona, Arizona Health Sciences Centeren
dc.identifier.journalBMC Ophthalmologyen
dc.description.collectioninformationThis item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.en
dc.eprint.versionFinal published versionen
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