The existence of Th22, pure Th17 and Th1 cells in CIN and Cervical Cancer along with their frequency variation in different stages of cervical cancer

Persistent Link:
http://hdl.handle.net/10150/610273
Title:
The existence of Th22, pure Th17 and Th1 cells in CIN and Cervical Cancer along with their frequency variation in different stages of cervical cancer
Author:
Zhang, W.; Tian, X.; Mumtahana, F.; Jiao, J.; Zhang, T.; Croce, K. D.; Ma, D.; Kong, B.; Cui, B.
Affiliation:
Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University; Key Laboratory of Gynecologic Oncology, Qilu Hospital, Shandong University; Hematology Oncology Center, Qilu Hospital, Shandong University; Department of Obstetrics and Gynecology, Weifang Maternal and Child Health Hospital; Department of Molecular & Cellular Biology, University of Arizona
Issue Date:
2015
Publisher:
BioMed Central Ltd
Citation:
Zhang et al. BMC Cancer (2015) 15:717 DOI 10.1186/s12885-015-1767-y
Journal:
BMC Cancer
Rights:
© 2015 Zhang et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/)
Collection Information:
This item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.
Abstract:
BACKGROUND: Recently, it is found that T-helper (Th) 22 cells are involved in different types of autoimmune and tumor diseases. But, till now, no study has been carried out to understand the involvement of these cells in cervical cancer (CC). METHODS: Flow cytometry was used to determine the expression of interferon gamma (IFN-gamma), Interleukin-22 (IL-22), IL-17 in the peripheral blood of healthy controls (HC), CIN and cervical cancer patients. From peripheral blood mononuclear cells (PBMCs), mRNA expression levels of Aryl hydrocarbon receptor (AHR), RAR-related orphan receptor C (RORC), TNF-alpha and IL-6 were respectively determined. Using the method of ELISA, plasma concentrations of IL-22, IL-17 and TNF-alpha were examined. RESULTS: Th22 and Th17 cells were elevated in CC and CIN patients. Th1 cells and the plasma concentrations of IL-22 in CC patients were significantly increased compared with HC. In CC patients, an increased prevalence of Th22 cells was associated with lymph node metastases. There was a positive correlation between Th22 and Th17 cells, but an approximately negative correlation between Th22 and Th1 cells in CC patients. The mRNA expression of RORC, TNF-alpha and IL-6 was significantly high in CC patients. CONCLUSIONS: Our results indicate that there is a higher circulatory frequency of Th22, Th17 and Th1 cells in CC which may conjointly participate in the pathogenesis and growth of CC.
EISSN:
1471-2407
PubMed ID:
26474968
PubMed Central ID:
PMC4609069
DOI:
10.1186/s12885-015-1767-y [doi]
Keywords:
Cervical cancer; Th17; Th22; Th1; IL-22
Version:
Final published version
Additional Links:
http://www.biomedcentral.com/1471-2407/15/717

Full metadata record

DC FieldValue Language
dc.contributor.authorZhang, W.en
dc.contributor.authorTian, X.en
dc.contributor.authorMumtahana, F.en
dc.contributor.authorJiao, J.en
dc.contributor.authorZhang, T.en
dc.contributor.authorCroce, K. D.en
dc.contributor.authorMa, D.en
dc.contributor.authorKong, B.en
dc.contributor.authorCui, B.en
dc.date.accessioned2016-05-20T09:02:50Z-
dc.date.available2016-05-20T09:02:50Z-
dc.date.issued2015en
dc.identifier.citationZhang et al. BMC Cancer (2015) 15:717 DOI 10.1186/s12885-015-1767-yen
dc.identifier.pmid26474968en
dc.identifier.doi10.1186/s12885-015-1767-y [doi]en
dc.identifier.urihttp://hdl.handle.net/10150/610273-
dc.description.abstractBACKGROUND: Recently, it is found that T-helper (Th) 22 cells are involved in different types of autoimmune and tumor diseases. But, till now, no study has been carried out to understand the involvement of these cells in cervical cancer (CC). METHODS: Flow cytometry was used to determine the expression of interferon gamma (IFN-gamma), Interleukin-22 (IL-22), IL-17 in the peripheral blood of healthy controls (HC), CIN and cervical cancer patients. From peripheral blood mononuclear cells (PBMCs), mRNA expression levels of Aryl hydrocarbon receptor (AHR), RAR-related orphan receptor C (RORC), TNF-alpha and IL-6 were respectively determined. Using the method of ELISA, plasma concentrations of IL-22, IL-17 and TNF-alpha were examined. RESULTS: Th22 and Th17 cells were elevated in CC and CIN patients. Th1 cells and the plasma concentrations of IL-22 in CC patients were significantly increased compared with HC. In CC patients, an increased prevalence of Th22 cells was associated with lymph node metastases. There was a positive correlation between Th22 and Th17 cells, but an approximately negative correlation between Th22 and Th1 cells in CC patients. The mRNA expression of RORC, TNF-alpha and IL-6 was significantly high in CC patients. CONCLUSIONS: Our results indicate that there is a higher circulatory frequency of Th22, Th17 and Th1 cells in CC which may conjointly participate in the pathogenesis and growth of CC.en
dc.language.isoenen
dc.publisherBioMed Central Ltden
dc.relation.urlhttp://www.biomedcentral.com/1471-2407/15/717en
dc.rights© 2015 Zhang et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/)en
dc.subjectCervical canceren
dc.subjectTh17en
dc.subjectTh22en
dc.subjectTh1en
dc.subjectIL-22en
dc.titleThe existence of Th22, pure Th17 and Th1 cells in CIN and Cervical Cancer along with their frequency variation in different stages of cervical canceren
dc.typeArticleen
dc.identifier.eissn1471-2407en
dc.contributor.departmentDepartment of Obstetrics and Gynecology, Qilu Hospital, Shandong Universityen
dc.contributor.departmentKey Laboratory of Gynecologic Oncology, Qilu Hospital, Shandong Universityen
dc.contributor.departmentHematology Oncology Center, Qilu Hospital, Shandong Universityen
dc.contributor.departmentDepartment of Obstetrics and Gynecology, Weifang Maternal and Child Health Hospitalen
dc.contributor.departmentDepartment of Molecular & Cellular Biology, University of Arizonaen
dc.identifier.journalBMC Canceren
dc.identifier.pmcidPMC4609069en
dc.description.collectioninformationThis item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.en
dc.eprint.versionFinal published versionen

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