Molecular characterization of a naturally occurring intraspecific recombinant begomovirus with close relatives widespread in southern Arabia

Persistent Link:
http://hdl.handle.net/10150/610262
Title:
Molecular characterization of a naturally occurring intraspecific recombinant begomovirus with close relatives widespread in southern Arabia
Author:
Al-Saleh, Mohammed; Al-Shahwan, Ibrahim; Brown, Judith; Idris, Ali
Affiliation:
Department of Plant Protection, King Saud University, Riyadh, Saudi Arabia; School of Plant Sciences, The University of Arizona, Tucson, AZ, USA; Center for Desert Agriculture, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia
Issue Date:
2014
Publisher:
BioMed Central
Citation:
Al-Saleh et al. Virology Journal 2014, 11:103 http://www.virologyj.com/content/11/1/103
Journal:
Virology Journal
Rights:
© 2014 Al-Saleh et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0)
Collection Information:
This item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.
Abstract:
BACKGROUND:Tomato leaf curl Sudan virus (ToLCSDV) is a single-stranded DNA begomovirus of tomato that causes downward leaf curl, yellowing, and stunting. Leaf curl disease results in significant yield reduction in tomato crops in the Nile Basin. ToLCSDV symptoms resemble those caused by Tomato yellow leaf curl virus, a distinct and widespread begomovirus originating in the Middle East. In this study, tomato samples exhibiting leaf curl symptoms were collected from Gezira, Sudan. The associated viral genome was molecularly characterized, analyzed phylogenetically, and an infectious clone for one isolate was constructed.FINDINGS:The complete genomes for five newly discovered variants of ToLCSDV, ranging in size from 2765 to 2767-bp, were cloned and sequenced, and subjected to pairwise and phylogenetic analyses. Pairwise analysis indicated that the five Gezira isolates shared 97-100% nucleotide identity with each other. Further, these variants of ToLCSDV shared their highest nucleotide identity at 96-98%, 91-95%, 91-92%, and 91-92% with the Shambat, Gezira, Oman and Yemen strains of ToLCSDV, respectively. Based on the high maximum nucleotide identities shared between these ToLCSDV variants from Gezira and other previously recognized members of this taxonomic group, they are considered isolates of the Shambat strain of ToLCSDV. Analysis of the complete genome sequence for these new variants revealed that they were naturally occurring recombinants between two previously reported strains of ToLCSDV. Finally, a dimeric clone constructed from one representative ToLCSV genome from Gezira was shown to be infectious following inoculation to tomato and N. benthamiana plants.CONCLUSION:Five new, naturally occurring recombinant begomovirus variants (>96% shared nt identity) were identified in tomato plants from Gezira in Sudan, and shown to be isolates of the Shambat strain of ToLCSDV. The cloned viral genome was infectious in N. benthamiana and tomato plants, and symptoms in tomato closely resembled those observed in field infected tomato plants, indicating the virus is the causal agent of the leaf curl disease. The symptoms that developed in tomato seedlings closely resembled those observed in field infected tomato plants, indicating that ToLCSDV is the causal agent of the leaf curl disease in Gezira.
EISSN:
1743-422X
DOI:
10.1186/1743-422X-11-103
Keywords:
Geminivirus; Tomato leaf curl Sudan virus; Virus variability; Whitefly vector
Version:
Final published version
Additional Links:
http://www.virologyj.com/content/11/1/103

Full metadata record

DC FieldValue Language
dc.contributor.authorAl-Saleh, Mohammeden
dc.contributor.authorAl-Shahwan, Ibrahimen
dc.contributor.authorBrown, Judithen
dc.contributor.authorIdris, Alien
dc.date.accessioned2016-05-20T09:02:31Z-
dc.date.available2016-05-20T09:02:31Z-
dc.date.issued2014en
dc.identifier.citationAl-Saleh et al. Virology Journal 2014, 11:103 http://www.virologyj.com/content/11/1/103en
dc.identifier.doi10.1186/1743-422X-11-103en
dc.identifier.urihttp://hdl.handle.net/10150/610262-
dc.description.abstractBACKGROUND:Tomato leaf curl Sudan virus (ToLCSDV) is a single-stranded DNA begomovirus of tomato that causes downward leaf curl, yellowing, and stunting. Leaf curl disease results in significant yield reduction in tomato crops in the Nile Basin. ToLCSDV symptoms resemble those caused by Tomato yellow leaf curl virus, a distinct and widespread begomovirus originating in the Middle East. In this study, tomato samples exhibiting leaf curl symptoms were collected from Gezira, Sudan. The associated viral genome was molecularly characterized, analyzed phylogenetically, and an infectious clone for one isolate was constructed.FINDINGS:The complete genomes for five newly discovered variants of ToLCSDV, ranging in size from 2765 to 2767-bp, were cloned and sequenced, and subjected to pairwise and phylogenetic analyses. Pairwise analysis indicated that the five Gezira isolates shared 97-100% nucleotide identity with each other. Further, these variants of ToLCSDV shared their highest nucleotide identity at 96-98%, 91-95%, 91-92%, and 91-92% with the Shambat, Gezira, Oman and Yemen strains of ToLCSDV, respectively. Based on the high maximum nucleotide identities shared between these ToLCSDV variants from Gezira and other previously recognized members of this taxonomic group, they are considered isolates of the Shambat strain of ToLCSDV. Analysis of the complete genome sequence for these new variants revealed that they were naturally occurring recombinants between two previously reported strains of ToLCSDV. Finally, a dimeric clone constructed from one representative ToLCSV genome from Gezira was shown to be infectious following inoculation to tomato and N. benthamiana plants.CONCLUSION:Five new, naturally occurring recombinant begomovirus variants (>96% shared nt identity) were identified in tomato plants from Gezira in Sudan, and shown to be isolates of the Shambat strain of ToLCSDV. The cloned viral genome was infectious in N. benthamiana and tomato plants, and symptoms in tomato closely resembled those observed in field infected tomato plants, indicating the virus is the causal agent of the leaf curl disease. The symptoms that developed in tomato seedlings closely resembled those observed in field infected tomato plants, indicating that ToLCSDV is the causal agent of the leaf curl disease in Gezira.en
dc.language.isoenen
dc.publisherBioMed Centralen
dc.relation.urlhttp://www.virologyj.com/content/11/1/103en
dc.rights© 2014 Al-Saleh et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0)en
dc.subjectGeminivirusen
dc.subjectTomato leaf curl Sudan virusen
dc.subjectVirus variabilityen
dc.subjectWhitefly vectoren
dc.titleMolecular characterization of a naturally occurring intraspecific recombinant begomovirus with close relatives widespread in southern Arabiaen
dc.typeArticleen
dc.identifier.eissn1743-422Xen
dc.contributor.departmentDepartment of Plant Protection, King Saud University, Riyadh, Saudi Arabiaen
dc.contributor.departmentSchool of Plant Sciences, The University of Arizona, Tucson, AZ, USAen
dc.contributor.departmentCenter for Desert Agriculture, King Abdullah University of Science and Technology, Thuwal, Saudi Arabiaen
dc.identifier.journalVirology Journalen
dc.description.collectioninformationThis item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.en
dc.eprint.versionFinal published versionen
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