Comparison of human adult stem cells from adipose tissue and bone marrow in the treatment of experimental autoimmune encephalomyelitis

Persistent Link:
http://hdl.handle.net/10150/610253
Title:
Comparison of human adult stem cells from adipose tissue and bone marrow in the treatment of experimental autoimmune encephalomyelitis
Author:
Semon, Julie; Maness, Catherine; Zhang, Xiujuan; Sharkey, Steven; Beuttler, Marc; Shah, Forum; Pandey, Amitabh; Gimble, Jeffrey; Zhang, Shijia; Scruggs, Brittni; Strong, Amy; Strong, Thomas; Bunnell, Bruce
Affiliation:
Center for Stem Cell Research and Regenerative Medicine, School of Medicine, Tulane University, 1430 Tulane Avenue, SL-99, New Orleans, LA 70112, USA; Department of Cell and Molecular Biology, School of Science and Engineering, Tulane University, 6400 Freret Street, New Orleans, LA 70118, USA; Department of Pharmacology, School of Medicine, Tulane University, 1430 Tulane Avenue, SL-83, New Orleans, LA 70112, USA; Stem Cell Biology Laboratory, Pennington Biomedical Research Center, Louisiana State University System, 6400 Perkins Road, Baton Rouge, LA 70808, USA
Issue Date:
2014
Publisher:
BioMed Central
Citation:
Semon et al. Stem Cell Research & Therapy 2014, 5:2 http://stemcellres.com/content/5/1/2
Journal:
Stem Cell Research & Therapy
Rights:
© 2014 Semon et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0)
Collection Information:
This item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.
Abstract:
INTRODUCTION:While administration of ex vivo culture-expanded stem cells has been used to study immunosuppressive mechanisms in multiple models of autoimmune diseases, less is known about the uncultured, nonexpanded stromal vascular fraction (SVF)-based therapy. The SVF is composed of a heterogeneous population of cells and has been used clinically to treat acute and chronic diseases, alleviating symptoms in a range of tissues and organs.METHODS:In this study, the ability of human SVF cells was compared with culture-expanded adipose stem cells (ASCs) and bone-derived marrow stromal cells (BMSCs) as a treatment of myelin oligodendrocyte glycoprotein (35-55)-induced experimental autoimmune encephalitis in C57Bl/6J mice, a well-studied multiple sclerosis model (MS). A total of 1x106 BMSCs, ASCs, or SVF cells were administered intraperitoneally concomitantly with the induction of disease. Mice were monitored daily for clinical signs of disease by three independent, blinded investigators and rated on a scale of 0 to 5. Spinal cords were obtained after euthanasia at day 30 and processed for histological staining using luxol fast blue, toluidine blue, and hematoxylin and eosin to measure myelin and infiltrating immune cells. Blood was collected from mice at day 30 and analyzed by enzyme-linked immunosorbent assay to measure serum levels of inflammatory cytokines.RESULTS:The data indicate that intraperitoneal administration of all cell types significantly ameliorates the severity of disease. Furthermore, the data also demonstrate, for the first time, that the SVF was as effective as the more commonly cultured BMSCs and ASCs in an MS model. All cell therapies also demonstrated a similar reduction in tissue damage, inflammatory infiltrates, and sera levels of IFNgamma and IL-12. While IFNgamma levels were reduced to comparable levels between treatment groups, levels of IL-12 were significantly lower in SVF-treated than BMSC-treated or ASC-treated mice.CONCLUSIONS:Based on these data, it is evident that SVF cells have relevant therapeutic potential in an animal model of chronic MS and might represent a valuable tool for stem cell-based therapy in chronic inflammatory disease of the central nervous system. SVF offers advantages of direct and rapid isolation procedure in a xenobiotic-free environment.
EISSN:
1757-6512
DOI:
10.1186/scrt391
Version:
Final published version
Additional Links:
http://stemcellres.com/content/5/1/2

Full metadata record

DC FieldValue Language
dc.contributor.authorSemon, Julieen
dc.contributor.authorManess, Catherineen
dc.contributor.authorZhang, Xiujuanen
dc.contributor.authorSharkey, Stevenen
dc.contributor.authorBeuttler, Marcen
dc.contributor.authorShah, Forumen
dc.contributor.authorPandey, Amitabhen
dc.contributor.authorGimble, Jeffreyen
dc.contributor.authorZhang, Shijiaen
dc.contributor.authorScruggs, Brittnien
dc.contributor.authorStrong, Amyen
dc.contributor.authorStrong, Thomasen
dc.contributor.authorBunnell, Bruceen
dc.date.accessioned2016-05-20T09:02:16Z-
dc.date.available2016-05-20T09:02:16Z-
dc.date.issued2014en
dc.identifier.citationSemon et al. Stem Cell Research & Therapy 2014, 5:2 http://stemcellres.com/content/5/1/2en
dc.identifier.doi10.1186/scrt391en
dc.identifier.urihttp://hdl.handle.net/10150/610253-
dc.description.abstractINTRODUCTION:While administration of ex vivo culture-expanded stem cells has been used to study immunosuppressive mechanisms in multiple models of autoimmune diseases, less is known about the uncultured, nonexpanded stromal vascular fraction (SVF)-based therapy. The SVF is composed of a heterogeneous population of cells and has been used clinically to treat acute and chronic diseases, alleviating symptoms in a range of tissues and organs.METHODS:In this study, the ability of human SVF cells was compared with culture-expanded adipose stem cells (ASCs) and bone-derived marrow stromal cells (BMSCs) as a treatment of myelin oligodendrocyte glycoprotein (35-55)-induced experimental autoimmune encephalitis in C57Bl/6J mice, a well-studied multiple sclerosis model (MS). A total of 1x106 BMSCs, ASCs, or SVF cells were administered intraperitoneally concomitantly with the induction of disease. Mice were monitored daily for clinical signs of disease by three independent, blinded investigators and rated on a scale of 0 to 5. Spinal cords were obtained after euthanasia at day 30 and processed for histological staining using luxol fast blue, toluidine blue, and hematoxylin and eosin to measure myelin and infiltrating immune cells. Blood was collected from mice at day 30 and analyzed by enzyme-linked immunosorbent assay to measure serum levels of inflammatory cytokines.RESULTS:The data indicate that intraperitoneal administration of all cell types significantly ameliorates the severity of disease. Furthermore, the data also demonstrate, for the first time, that the SVF was as effective as the more commonly cultured BMSCs and ASCs in an MS model. All cell therapies also demonstrated a similar reduction in tissue damage, inflammatory infiltrates, and sera levels of IFNgamma and IL-12. While IFNgamma levels were reduced to comparable levels between treatment groups, levels of IL-12 were significantly lower in SVF-treated than BMSC-treated or ASC-treated mice.CONCLUSIONS:Based on these data, it is evident that SVF cells have relevant therapeutic potential in an animal model of chronic MS and might represent a valuable tool for stem cell-based therapy in chronic inflammatory disease of the central nervous system. SVF offers advantages of direct and rapid isolation procedure in a xenobiotic-free environment.en
dc.language.isoenen
dc.publisherBioMed Centralen
dc.relation.urlhttp://stemcellres.com/content/5/1/2en
dc.rights© 2014 Semon et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0)en
dc.titleComparison of human adult stem cells from adipose tissue and bone marrow in the treatment of experimental autoimmune encephalomyelitisen
dc.typeArticleen
dc.identifier.eissn1757-6512en
dc.contributor.departmentCenter for Stem Cell Research and Regenerative Medicine, School of Medicine, Tulane University, 1430 Tulane Avenue, SL-99, New Orleans, LA 70112, USAen
dc.contributor.departmentDepartment of Cell and Molecular Biology, School of Science and Engineering, Tulane University, 6400 Freret Street, New Orleans, LA 70118, USAen
dc.contributor.departmentDepartment of Pharmacology, School of Medicine, Tulane University, 1430 Tulane Avenue, SL-83, New Orleans, LA 70112, USAen
dc.contributor.departmentStem Cell Biology Laboratory, Pennington Biomedical Research Center, Louisiana State University System, 6400 Perkins Road, Baton Rouge, LA 70808, USAen
dc.identifier.journalStem Cell Research & Therapyen
dc.description.collectioninformationThis item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.en
dc.eprint.versionFinal published versionen
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