Cell type-specific characterization of nuclear DNA contents within complex tissues and organs

Persistent Link:
http://hdl.handle.net/10150/610234
Title:
Cell type-specific characterization of nuclear DNA contents within complex tissues and organs
Author:
Zhang, Changqing; Gong, Fang; Lambert, Georgina; Galbraith, David
Affiliation:
Department of Plant Sciences, The University of Arizona, Tucson, Arizona, 85721, USA; Operon Biotechnologies, Inc., 2705 Artie Street Bldg. 400, Ste. 27, Huntsville, AL 35805, USA
Issue Date:
2005
Publisher:
BioMed Central
Citation:
Plant Methods 2005, 1:7 doi:10.1186/1746-4811-1-7
Journal:
Plant Methods
Rights:
© 2005 Zhang et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0)
Collection Information:
This item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.
Abstract:
BACKGROUND:Eukaryotic organisms are defined by the presence of a nucleus, which encloses the chromosomal DNA, and is characterized by its DNA content (C-value). Complex eukaryotic organisms contain organs and tissues that comprise interspersions of different cell types, within which polysomaty, endoreduplication, and cell cycle arrest is frequently observed. Little is known about the distribution of C-values across different cell types within these organs and tissues.RESULTS:We have developed, and describe here, a method to precisely define the C-value status within any specific cell type within complex organs and tissues of plants. We illustrate the application of this method to Arabidopsis thaliana, specifically focusing on the different cell types found within the root.CONCLUSION:The method accurately and conveniently charts C-value within specific cell types, and provides novel insight into developmental processes. The method is, in principle, applicable to any transformable organism, including mammals, within which cell type specificity of regulation of endoreduplication, of polysomaty, and of cell cycle arrest is suspected.
EISSN:
1746-4811
DOI:
10.1186/1746-4811-1-7
Version:
Final published version
Additional Links:
http://www.plantmethods.com/content/1/1/7

Full metadata record

DC FieldValue Language
dc.contributor.authorZhang, Changqingen
dc.contributor.authorGong, Fangen
dc.contributor.authorLambert, Georginaen
dc.contributor.authorGalbraith, Daviden
dc.date.accessioned2016-05-20T09:01:48Z-
dc.date.available2016-05-20T09:01:48Z-
dc.date.issued2005en
dc.identifier.citationPlant Methods 2005, 1:7 doi:10.1186/1746-4811-1-7en
dc.identifier.doi10.1186/1746-4811-1-7en
dc.identifier.urihttp://hdl.handle.net/10150/610234-
dc.description.abstractBACKGROUND:Eukaryotic organisms are defined by the presence of a nucleus, which encloses the chromosomal DNA, and is characterized by its DNA content (C-value). Complex eukaryotic organisms contain organs and tissues that comprise interspersions of different cell types, within which polysomaty, endoreduplication, and cell cycle arrest is frequently observed. Little is known about the distribution of C-values across different cell types within these organs and tissues.RESULTS:We have developed, and describe here, a method to precisely define the C-value status within any specific cell type within complex organs and tissues of plants. We illustrate the application of this method to Arabidopsis thaliana, specifically focusing on the different cell types found within the root.CONCLUSION:The method accurately and conveniently charts C-value within specific cell types, and provides novel insight into developmental processes. The method is, in principle, applicable to any transformable organism, including mammals, within which cell type specificity of regulation of endoreduplication, of polysomaty, and of cell cycle arrest is suspected.en
dc.language.isoenen
dc.publisherBioMed Centralen
dc.relation.urlhttp://www.plantmethods.com/content/1/1/7en
dc.rights© 2005 Zhang et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0)en
dc.titleCell type-specific characterization of nuclear DNA contents within complex tissues and organsen
dc.typeArticleen
dc.identifier.eissn1746-4811en
dc.contributor.departmentDepartment of Plant Sciences, The University of Arizona, Tucson, Arizona, 85721, USAen
dc.contributor.departmentOperon Biotechnologies, Inc., 2705 Artie Street Bldg. 400, Ste. 27, Huntsville, AL 35805, USAen
dc.identifier.journalPlant Methodsen
dc.description.collectioninformationThis item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.en
dc.eprint.versionFinal published versionen
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