Cactus pear: a natural product in cancer chemoprevention

Persistent Link:
http://hdl.handle.net/10150/610227
Title:
Cactus pear: a natural product in cancer chemoprevention
Author:
Zou, Da-ming; Brewer, Molly; Garcia, Francisco; Feugang, Jean; Wang, Jian; Zang, Roungyu; Liu, Huaguang; Zou, Changping
Affiliation:
Department of Obstetrics and Gynecology, Arizona Health Sciences Center, University of Arizona, Tucson, Arizona 85724, USA; Division of Gynecologic Oncology, Arizona Cancer Center, Tucson, Arizona 85724, USA; Department of Gynecologic Oncology, Fudan Univeristy, Shanghai, 200032, China; Guangxi Medical University, Guangxi, 532021, China
Issue Date:
2005
Publisher:
BioMed Central
Citation:
Nutrition Journal 2005, 4:25 doi:10.1186/1475-2891-4-25
Journal:
Nutrition Journal
Rights:
© 2005 Zou et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0)
Collection Information:
This item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.
Abstract:
BACKGROUND:Cancer chemoprevention is a new approach in cancer prevention, in which chemical agents are used to prevent cancer in normal and/or high-risk populations. Although chemoprevention has shown promise in some epithelial cancers, currently available preventive agents are limited and the agents are costly, generally with side effects. Natural products, such as grape seed, green tea, and certain herbs have demonstrated anti-cancer effects. To find a natural product that can be used in chemoprevention of cancer, we tested Arizona cactus fruit solution, the aqueous extracts of cactus pear, for its anti-cancer effects in cultured cells and in an animal model.METHOD:Aqueous extracts of cactus pear were used to treat immortalized ovarian and cervical epithelial cells, as well as ovarian, cervical, and bladder cancer cells. Aqueous extracts of cactus pear were used at six concentrations (0, 0.5, 1, 5, 10 or 25%) to treat cells for 1, 3, or 5 days. Growth inhibition, apoptosis induction, and cell cycle changes were analyzed in the cultured cells; the suppression of tumor growth in nude mice was evaluated and compared with the effect of a synthetic retinoid N-(4-hydroxyphernyl) retinamide (4-HPR), which is currently used as a chemoprevention agent. Immunohistochemistry staining of tissue samples from animal tumors was performed to examine the gene expression.RESULTS:Cells exposed to cactus pear extracts had a significant increase in apoptosis and growth inhibition in both immortalized epithelial cells and cancer cells in a dose- and time-dependent manner. It also affected cell cycle of cancer cells by increasing G1 and decreasing G2 and S phases. Both 4-HPR and cactus pear extracts significantly suppressed tumor growth in nude mice, increased annexin IV expression, and decreased VEGF expression.CONCLUSION:Arizona cactus pear extracts effectively inhibited cell growth in several different immortalized and cancer cell cultures, suppressed tumor growth in nude mice, and modulated expression of tumor-related genes. These effects were comparable with those caused by a synthetic retinoid currently used in chemoprevention trials. The mechanism of the anti-cancer effects of cactus pear extracts needs to be further studied.
EISSN:
1475-2891
DOI:
10.1186/1475-2891-4-25
Version:
Final published version
Additional Links:
http://www.nutritionj.com/content/4/1/25

Full metadata record

DC FieldValue Language
dc.contributor.authorZou, Da-mingen
dc.contributor.authorBrewer, Mollyen
dc.contributor.authorGarcia, Franciscoen
dc.contributor.authorFeugang, Jeanen
dc.contributor.authorWang, Jianen
dc.contributor.authorZang, Roungyuen
dc.contributor.authorLiu, Huaguangen
dc.contributor.authorZou, Changpingen
dc.date.accessioned2016-05-20T09:01:35Z-
dc.date.available2016-05-20T09:01:35Z-
dc.date.issued2005en
dc.identifier.citationNutrition Journal 2005, 4:25 doi:10.1186/1475-2891-4-25en
dc.identifier.doi10.1186/1475-2891-4-25en
dc.identifier.urihttp://hdl.handle.net/10150/610227-
dc.description.abstractBACKGROUND:Cancer chemoprevention is a new approach in cancer prevention, in which chemical agents are used to prevent cancer in normal and/or high-risk populations. Although chemoprevention has shown promise in some epithelial cancers, currently available preventive agents are limited and the agents are costly, generally with side effects. Natural products, such as grape seed, green tea, and certain herbs have demonstrated anti-cancer effects. To find a natural product that can be used in chemoprevention of cancer, we tested Arizona cactus fruit solution, the aqueous extracts of cactus pear, for its anti-cancer effects in cultured cells and in an animal model.METHOD:Aqueous extracts of cactus pear were used to treat immortalized ovarian and cervical epithelial cells, as well as ovarian, cervical, and bladder cancer cells. Aqueous extracts of cactus pear were used at six concentrations (0, 0.5, 1, 5, 10 or 25%) to treat cells for 1, 3, or 5 days. Growth inhibition, apoptosis induction, and cell cycle changes were analyzed in the cultured cellsen
dc.description.abstractthe suppression of tumor growth in nude mice was evaluated and compared with the effect of a synthetic retinoid N-(4-hydroxyphernyl) retinamide (4-HPR), which is currently used as a chemoprevention agent. Immunohistochemistry staining of tissue samples from animal tumors was performed to examine the gene expression.RESULTS:Cells exposed to cactus pear extracts had a significant increase in apoptosis and growth inhibition in both immortalized epithelial cells and cancer cells in a dose- and time-dependent manner. It also affected cell cycle of cancer cells by increasing G1 and decreasing G2 and S phases. Both 4-HPR and cactus pear extracts significantly suppressed tumor growth in nude mice, increased annexin IV expression, and decreased VEGF expression.CONCLUSION:Arizona cactus pear extracts effectively inhibited cell growth in several different immortalized and cancer cell cultures, suppressed tumor growth in nude mice, and modulated expression of tumor-related genes. These effects were comparable with those caused by a synthetic retinoid currently used in chemoprevention trials. The mechanism of the anti-cancer effects of cactus pear extracts needs to be further studied.en
dc.language.isoenen
dc.publisherBioMed Centralen
dc.relation.urlhttp://www.nutritionj.com/content/4/1/25en
dc.rights© 2005 Zou et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0)en
dc.titleCactus pear: a natural product in cancer chemopreventionen
dc.typeArticleen
dc.identifier.eissn1475-2891en
dc.contributor.departmentDepartment of Obstetrics and Gynecology, Arizona Health Sciences Center, University of Arizona, Tucson, Arizona 85724, USAen
dc.contributor.departmentDivision of Gynecologic Oncology, Arizona Cancer Center, Tucson, Arizona 85724, USAen
dc.contributor.departmentDepartment of Gynecologic Oncology, Fudan Univeristy, Shanghai, 200032, Chinaen
dc.contributor.departmentGuangxi Medical University, Guangxi, 532021, Chinaen
dc.identifier.journalNutrition Journalen
dc.description.collectioninformationThis item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.en
dc.eprint.versionFinal published versionen
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