Regulation of colony stimulating factor-1 expression and ovarian cancer cell behavior in vitro by miR-128 and miR-152

Persistent Link:
http://hdl.handle.net/10150/610206
Title:
Regulation of colony stimulating factor-1 expression and ovarian cancer cell behavior in vitro by miR-128 and miR-152
Author:
Woo, Ho-Hyung; Laszlo, Csaba; Greco, Stephen; Chambers, Setsuko
Affiliation:
Arizona Cancer Center, University of Arizona, Tucson, AZ 85724, USA
Issue Date:
2012
Publisher:
BioMed Central
Citation:
Woo et al. Molecular Cancer 2012, 11:58 http://www.molecular-cancer.com/content/11/1/58
Journal:
Molecular Cancer
Rights:
© 2012 Woo et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0)
Collection Information:
This item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.
Abstract:
BACKGROUND:Colony stimulating factor-1 (CSF-1) plays an important role in ovarian cancer biology and as a prognostic factor in ovarian cancer. Elevated levels of CSF-1 promote progression of ovarian cancer, by binding to CSF-1R (the tyrosine kinase receptor encoded by c-fms proto-oncogene).Post-transcriptional regulation of CSF-1 mRNA by its 3' untranslated region (3'UTR) has been studied previously. Several cis-acting elements in 3'UTR are involved in post-transcriptional regulation of CSF-1 mRNA. These include conserved protein-binding motifs as well as miRNA targets. miRNAs are 21-23nt single strand RNA which bind the complementary sequences in mRNAs, suppressing translation and enhancing mRNA degradation.RESULTS:In this report, we investigate the effect of miRNAs on post-transcriptional regulation of CSF-1 mRNA in human ovarian cancer. Bioinformatics analysis predicts at least 14 miRNAs targeting CSF-1 mRNA 3'UTR. By mutations in putative miRNA targets in CSF-1 mRNA 3'UTR, we identified a common target for both miR-128 and miR-152. We have also found that both miR-128 and miR-152 down-regulate CSF-1 mRNA and protein expression in ovarian cancer cells leading to decreased cell motility and adhesion in vitro, two major aspects of the metastatic potential of cancer cells.CONCLUSION:The major CSF-1 mRNA 3'UTR contains a common miRNA target which is involved in post-transcriptional regulation of CSF-1. Our results provide the evidence for a mechanism by which miR-128 and miR-152 down-regulate CSF-1, an important regulator of ovarian cancer.
EISSN:
1476-4598
DOI:
10.1186/1476-4598-11-58
Keywords:
miR-128; miR-152; CSF-1 mRNA; Post-transcriptional regulation; motility and adhesion
Version:
Final published version
Additional Links:
http://www.molecular-cancer.com/content/11/1/58

Full metadata record

DC FieldValue Language
dc.contributor.authorWoo, Ho-Hyungen
dc.contributor.authorLaszlo, Csabaen
dc.contributor.authorGreco, Stephenen
dc.contributor.authorChambers, Setsukoen
dc.date.accessioned2016-05-20T09:01:04Z-
dc.date.available2016-05-20T09:01:04Z-
dc.date.issued2012en
dc.identifier.citationWoo et al. Molecular Cancer 2012, 11:58 http://www.molecular-cancer.com/content/11/1/58en
dc.identifier.doi10.1186/1476-4598-11-58en
dc.identifier.urihttp://hdl.handle.net/10150/610206-
dc.description.abstractBACKGROUND:Colony stimulating factor-1 (CSF-1) plays an important role in ovarian cancer biology and as a prognostic factor in ovarian cancer. Elevated levels of CSF-1 promote progression of ovarian cancer, by binding to CSF-1R (the tyrosine kinase receptor encoded by c-fms proto-oncogene).Post-transcriptional regulation of CSF-1 mRNA by its 3' untranslated region (3'UTR) has been studied previously. Several cis-acting elements in 3'UTR are involved in post-transcriptional regulation of CSF-1 mRNA. These include conserved protein-binding motifs as well as miRNA targets. miRNAs are 21-23nt single strand RNA which bind the complementary sequences in mRNAs, suppressing translation and enhancing mRNA degradation.RESULTS:In this report, we investigate the effect of miRNAs on post-transcriptional regulation of CSF-1 mRNA in human ovarian cancer. Bioinformatics analysis predicts at least 14 miRNAs targeting CSF-1 mRNA 3'UTR. By mutations in putative miRNA targets in CSF-1 mRNA 3'UTR, we identified a common target for both miR-128 and miR-152. We have also found that both miR-128 and miR-152 down-regulate CSF-1 mRNA and protein expression in ovarian cancer cells leading to decreased cell motility and adhesion in vitro, two major aspects of the metastatic potential of cancer cells.CONCLUSION:The major CSF-1 mRNA 3'UTR contains a common miRNA target which is involved in post-transcriptional regulation of CSF-1. Our results provide the evidence for a mechanism by which miR-128 and miR-152 down-regulate CSF-1, an important regulator of ovarian cancer.en
dc.language.isoenen
dc.publisherBioMed Centralen
dc.relation.urlhttp://www.molecular-cancer.com/content/11/1/58en
dc.rights© 2012 Woo et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0)en
dc.subjectmiR-128en
dc.subjectmiR-152en
dc.subjectCSF-1 mRNAen
dc.subjectPost-transcriptional regulationen
dc.subjectmotility and adhesionen
dc.titleRegulation of colony stimulating factor-1 expression and ovarian cancer cell behavior in vitro by miR-128 and miR-152en
dc.typeArticleen
dc.identifier.eissn1476-4598en
dc.contributor.departmentArizona Cancer Center, University of Arizona, Tucson, AZ 85724, USAen
dc.identifier.journalMolecular Canceren
dc.description.collectioninformationThis item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.en
dc.eprint.versionFinal published versionen
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