Prostate transglutaminase (TGase-4, TGaseP) enhances the adhesion of prostate cancer cells to extracellular matrix, the potential role of TGase-core domain

Persistent Link:
http://hdl.handle.net/10150/610200
Title:
Prostate transglutaminase (TGase-4, TGaseP) enhances the adhesion of prostate cancer cells to extracellular matrix, the potential role of TGase-core domain
Author:
Jiang, Wen; Ye, Lin; Sanders, Andrew; Ruge, Fiona; Kynaston, Howard; Ablin, Richard; Mason, Malcolm
Affiliation:
Metastasis and Angiogenesis Research Group, Cardiff University School of Medicine, Heath Park, Cardiff CF14 4XN, UK; Department of Pathology, Health Sciences Center, University of Arizona College of Medicine and the Arizona Cancer Center, 1501 N. Campbell Avenue, P.O. Box 245043, Tucson, AZ 85724-5043, USA
Issue Date:
2013
Publisher:
BioMed Central
Citation:
Jiang et al. Journal of Translational Medicine 2013, 11:269 http://www.translational-medicine.com/content/11/1/269
Journal:
Journal of Translational Medicine
Rights:
© 2013 Jiang et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0)
Collection Information:
This item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.
Abstract:
BACKGROUND:Transglutaminase-4 (TGase-4), also known as the Prostate Transglutaminase, is an enzyme found to be expressed predominately in the prostate gland. The protein has been recently reported to influence the migration and invasiveness of prostate cancer cells. The present study aimed to investigate the influence of TGase-4 on cell-matrix adhesion and search for the candidate active domains] within the protein.METHODS:Human prostate cancer cell lines and prostate tissues were used. Plasmids that encoded different domains and full length of TGase-4 were constructed and used to generate sublines that expressed different domains. The impact of TGase-4 on in vitro cell-matrix adhesion, cell migration, growth and in vivo growth were investigated. Interactions between TGase-4 and focal adhesion complex proteins were investigated using immunoprecipitation, immunofluorescence and phosphospecific antibodies.RESULTS:TGase-4 markedly increased cell-matrix adhesion and cellular migration, and resulted in a rapid growth of prostate tumours in vivo. This effect resided in the Core-domain of the TGase-4 protein. TGase-4 was found to co-precipitate and co-localise with focal adhesion kinase (FAK) and paxillin, in cells, human prostate tissues and tumour xenografts. FAK small inhibitor was able to block the action mediated by TGase-4 and TGase-4 core domain.CONCLUSION:TGase-4 is an important regulator of cell-matrix adhesion of prostate cancer cells. This effect is predominately mediated by its core domain and requires the participation of focal adhesion complex proteins.
EISSN:
1479-5876
DOI:
10.1186/1479-5876-11-269
Keywords:
Transglutaminase; Transglutaminase-4; Cell-matrix adhesion; Focal adhesion kinase; Paxillin; Integrins; Electric cell sensing; Prostate cancer
Version:
Final published version
Additional Links:
http://www.translational-medicine.com/content/11/1/269

Full metadata record

DC FieldValue Language
dc.contributor.authorJiang, Wenen
dc.contributor.authorYe, Linen
dc.contributor.authorSanders, Andrewen
dc.contributor.authorRuge, Fionaen
dc.contributor.authorKynaston, Howarden
dc.contributor.authorAblin, Richarden
dc.contributor.authorMason, Malcolmen
dc.date.accessioned2016-05-20T09:00:55Z-
dc.date.available2016-05-20T09:00:55Z-
dc.date.issued2013en
dc.identifier.citationJiang et al. Journal of Translational Medicine 2013, 11:269 http://www.translational-medicine.com/content/11/1/269en
dc.identifier.doi10.1186/1479-5876-11-269en
dc.identifier.urihttp://hdl.handle.net/10150/610200-
dc.description.abstractBACKGROUND:Transglutaminase-4 (TGase-4), also known as the Prostate Transglutaminase, is an enzyme found to be expressed predominately in the prostate gland. The protein has been recently reported to influence the migration and invasiveness of prostate cancer cells. The present study aimed to investigate the influence of TGase-4 on cell-matrix adhesion and search for the candidate active domains] within the protein.METHODS:Human prostate cancer cell lines and prostate tissues were used. Plasmids that encoded different domains and full length of TGase-4 were constructed and used to generate sublines that expressed different domains. The impact of TGase-4 on in vitro cell-matrix adhesion, cell migration, growth and in vivo growth were investigated. Interactions between TGase-4 and focal adhesion complex proteins were investigated using immunoprecipitation, immunofluorescence and phosphospecific antibodies.RESULTS:TGase-4 markedly increased cell-matrix adhesion and cellular migration, and resulted in a rapid growth of prostate tumours in vivo. This effect resided in the Core-domain of the TGase-4 protein. TGase-4 was found to co-precipitate and co-localise with focal adhesion kinase (FAK) and paxillin, in cells, human prostate tissues and tumour xenografts. FAK small inhibitor was able to block the action mediated by TGase-4 and TGase-4 core domain.CONCLUSION:TGase-4 is an important regulator of cell-matrix adhesion of prostate cancer cells. This effect is predominately mediated by its core domain and requires the participation of focal adhesion complex proteins.en
dc.language.isoenen
dc.publisherBioMed Centralen
dc.relation.urlhttp://www.translational-medicine.com/content/11/1/269en
dc.rights© 2013 Jiang et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0)en
dc.subjectTransglutaminaseen
dc.subjectTransglutaminase-4en
dc.subjectCell-matrix adhesionen
dc.subjectFocal adhesion kinaseen
dc.subjectPaxillinen
dc.subjectIntegrinsen
dc.subjectElectric cell sensingen
dc.subjectProstate canceren
dc.titleProstate transglutaminase (TGase-4, TGaseP) enhances the adhesion of prostate cancer cells to extracellular matrix, the potential role of TGase-core domainen
dc.typeArticleen
dc.identifier.eissn1479-5876en
dc.contributor.departmentMetastasis and Angiogenesis Research Group, Cardiff University School of Medicine, Heath Park, Cardiff CF14 4XN, UKen
dc.contributor.departmentDepartment of Pathology, Health Sciences Center, University of Arizona College of Medicine and the Arizona Cancer Center, 1501 N. Campbell Avenue, P.O. Box 245043, Tucson, AZ 85724-5043, USAen
dc.identifier.journalJournal of Translational Medicineen
dc.description.collectioninformationThis item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.en
dc.eprint.versionFinal published versionen
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