Hydrodebridement of wounds: effectiveness in reducing wound bacterial contamination and potential for air bacterial contamination

Persistent Link:
http://hdl.handle.net/10150/610180
Title:
Hydrodebridement of wounds: effectiveness in reducing wound bacterial contamination and potential for air bacterial contamination
Author:
Bowling, Frank; Stickings, Daryl; Edwards-Jones, Valerie; Armstrong, David; Boulton, Andrew
Affiliation:
Department of Medicine Manchester Royal Infirmary, University of Manchester, Manchester, UK; Manchester Foot Clinic, Manchester Community Health, Manchester, UK; Department of Clinical Microbiology, Manchester Metropolitan University, Manchester, UK; Department of Surgery, University of Arizona College of Medicine, Tucson, AZ, USA
Issue Date:
2009
Publisher:
BioMed Central
Citation:
Journal of Foot and Ankle Research 2009, 2:13 doi:10.1186/1757-1146-2-13
Journal:
Journal of Foot and Ankle Research
Rights:
© 2009 Bowling et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0)
Collection Information:
This item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.
Abstract:
BACKGROUND:The purpose of this study was to assess the level of air contamination with bacteria after surgical hydrodebridement and to determine the effectiveness of hydro surgery on bacterial reduction of a simulated infected wound.METHODS:Four porcine samples were scored then infected with a broth culture containing a variety of organisms and incubated at 37degreesC for 24 hours. The infected samples were then debrided with the hydro surgery tool (Versajet, Smith and Nephew, Largo, Florida, USA). Samples were taken for microbiology, histology and scanning electron microscopy pre-infection, post infection and post debridement. Air bacterial contamination was evaluated before, during and after debridement by using active and passive methods; for active sampling the SAS-Super 90 air sampler was used, for passive sampling settle plates were located at set distances around the clinic room.RESULTS:There was no statistically significant reduction in bacterial contamination of the porcine samples post hydrodebridement. Analysis of the passive sampling showed a significant (p < 0.001) increase in microbial counts post hydrodebridement. Levels ranging from 950 colony forming units per meter cubed (CFUs/m3) to 16780 CFUs/m3 were observed with active sampling of the air whilst using hydro surgery equipment compared with a basal count of 582 CFUs/m3. During removal of the wound dressing, a significant increase was observed relative to basal counts (p < 0.05). Microbial load of the air samples was still significantly raised 1 hour post-therapy.CONCLUSION:The results suggest a significant increase in bacterial air contamination both by active sampling and passive sampling. We believe that action might be taken to mitigate fallout in the settings in which this technique is used.
EISSN:
1757-1146
DOI:
10.1186/1757-1146-2-13
Version:
Final published version
Additional Links:
http://www.jfootankleres.com/content/2/1/13

Full metadata record

DC FieldValue Language
dc.contributor.authorBowling, Franken
dc.contributor.authorStickings, Darylen
dc.contributor.authorEdwards-Jones, Valerieen
dc.contributor.authorArmstrong, Daviden
dc.contributor.authorBoulton, Andrewen
dc.date.accessioned2016-05-20T09:00:27Z-
dc.date.available2016-05-20T09:00:27Z-
dc.date.issued2009en
dc.identifier.citationJournal of Foot and Ankle Research 2009, 2:13 doi:10.1186/1757-1146-2-13en
dc.identifier.doi10.1186/1757-1146-2-13en
dc.identifier.urihttp://hdl.handle.net/10150/610180-
dc.description.abstractBACKGROUND:The purpose of this study was to assess the level of air contamination with bacteria after surgical hydrodebridement and to determine the effectiveness of hydro surgery on bacterial reduction of a simulated infected wound.METHODS:Four porcine samples were scored then infected with a broth culture containing a variety of organisms and incubated at 37degreesC for 24 hours. The infected samples were then debrided with the hydro surgery tool (Versajet, Smith and Nephew, Largo, Florida, USA). Samples were taken for microbiology, histology and scanning electron microscopy pre-infection, post infection and post debridement. Air bacterial contamination was evaluated before, during and after debridement by using active and passive methodsen
dc.description.abstractfor active sampling the SAS-Super 90 air sampler was used, for passive sampling settle plates were located at set distances around the clinic room.RESULTS:There was no statistically significant reduction in bacterial contamination of the porcine samples post hydrodebridement. Analysis of the passive sampling showed a significant (p < 0.001) increase in microbial counts post hydrodebridement. Levels ranging from 950 colony forming units per meter cubed (CFUs/m3) to 16780 CFUs/m3 were observed with active sampling of the air whilst using hydro surgery equipment compared with a basal count of 582 CFUs/m3. During removal of the wound dressing, a significant increase was observed relative to basal counts (p < 0.05). Microbial load of the air samples was still significantly raised 1 hour post-therapy.CONCLUSION:The results suggest a significant increase in bacterial air contamination both by active sampling and passive sampling. We believe that action might be taken to mitigate fallout in the settings in which this technique is used.en
dc.language.isoenen
dc.publisherBioMed Centralen
dc.relation.urlhttp://www.jfootankleres.com/content/2/1/13en
dc.rights© 2009 Bowling et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0)en
dc.titleHydrodebridement of wounds: effectiveness in reducing wound bacterial contamination and potential for air bacterial contaminationen
dc.typeArticleen
dc.identifier.eissn1757-1146en
dc.contributor.departmentDepartment of Medicine Manchester Royal Infirmary, University of Manchester, Manchester, UKen
dc.contributor.departmentManchester Foot Clinic, Manchester Community Health, Manchester, UKen
dc.contributor.departmentDepartment of Clinical Microbiology, Manchester Metropolitan University, Manchester, UKen
dc.contributor.departmentDepartment of Surgery, University of Arizona College of Medicine, Tucson, AZ, USAen
dc.identifier.journalJournal of Foot and Ankle Researchen
dc.description.collectioninformationThis item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.en
dc.eprint.versionFinal published versionen
All Items in UA Campus Repository are protected by copyright, with all rights reserved, unless otherwise indicated.