Homoeolog-specific retention and use in allotetraploid Arabidopsis suecica depends on parent of origin and network partners

Persistent Link:
http://hdl.handle.net/10150/610148
Title:
Homoeolog-specific retention and use in allotetraploid Arabidopsis suecica depends on parent of origin and network partners
Author:
Chang, Peter; Dilkes, Brian; McMahon, Michelle; Comai, Luca; Nuzhdin, Sergey
Affiliation:
Molecular and Computational Biology, University of Southern California, 1050 Childs Way, RRI 201, Los Angeles, CA 90089-2910, USA; Genome Center and Department of Plant Biology, University of California at Davis, 451 Health Services Drive, Davis, CA 95616, USA; Current address: Department of Horticulture and Landscape Architecture, Purdue University, 625 Agriculture Mall Drive, West Lafayette, IN 47907-2010, USA; School of Plant Sciences, University of Arizona, 1140 E. South Campus Drive, Forbes Building, Room 303, Tucson, AZ 85721-0036, USA
Issue Date:
2010
Publisher:
BioMed Central
Citation:
Chang et al. Genome Biology 2010, 11:R125 http://genomebiology.com/2010/11/12/R125
Journal:
Genome Biology
Rights:
© 2010 Chang et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0)
Collection Information:
This item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.
Abstract:
BACKGROUND:Allotetraploids carry pairs of diverged homoeologs for most genes. With the genome doubled in size, the number of putative interactions is enormous. This poses challenges on how to coordinate the two disparate genomes, and creates opportunities by enhancing the phenotypic variation. New combinations of alleles co-adapt and respond to new environmental pressures. Three stages of the allopolyploidization process - parental species divergence, hybridization, and genome duplication - have been well analyzed. The last stage of evolutionary adjustments remains mysterious.RESULTS:Homoeolog-specific retention and use were analyzed in Arabidopsis suecica (As), a species derived from A. thaliana (At) and A. arenosa (Aa) in a single event 12,000 to 300,000 years ago. We used 405,466 diagnostic features on tiling microarrays to recognize At and Aa contributions to the As genome and transcriptome: 324 genes lacked Aa contributions and 614 genes lacked At contributions within As. In leaf tissues, 3,458 genes preferentially expressed At homoeologs while 4,150 favored Aa homoeologs. These patterns were validated with resequencing. Genes with preferential use of Aa homoeologs were enriched for expression functions, consistent with the dominance of Aa transcription. Heterologous networks - mixed from At and Aa transcripts - were underrepresented.CONCLUSIONS:Thousands of deleted and silenced homoeologs in the genome of As were identified. Since heterologous networks may be compromised by interspecies incompatibilities, these networks evolve co-biases, expressing either only Aa or only At homoeologs. This progressive change towards predominantly pure parental networks might contribute to phenotypic variability and plasticity, and enable the species to exploit a larger range of environments.
DOI:
10.1186/gb-2010-11-12-r125
Version:
Final published version
Additional Links:
http://genomebiology.com/2010/11/12/R125

Full metadata record

DC FieldValue Language
dc.contributor.authorChang, Peteren
dc.contributor.authorDilkes, Brianen
dc.contributor.authorMcMahon, Michelleen
dc.contributor.authorComai, Lucaen
dc.contributor.authorNuzhdin, Sergeyen
dc.date.accessioned2016-05-20T08:59:37Z-
dc.date.available2016-05-20T08:59:37Z-
dc.date.issued2010en
dc.identifier.citationChang et al. Genome Biology 2010, 11:R125 http://genomebiology.com/2010/11/12/R125en
dc.identifier.doi10.1186/gb-2010-11-12-r125en
dc.identifier.urihttp://hdl.handle.net/10150/610148-
dc.description.abstractBACKGROUND:Allotetraploids carry pairs of diverged homoeologs for most genes. With the genome doubled in size, the number of putative interactions is enormous. This poses challenges on how to coordinate the two disparate genomes, and creates opportunities by enhancing the phenotypic variation. New combinations of alleles co-adapt and respond to new environmental pressures. Three stages of the allopolyploidization process - parental species divergence, hybridization, and genome duplication - have been well analyzed. The last stage of evolutionary adjustments remains mysterious.RESULTS:Homoeolog-specific retention and use were analyzed in Arabidopsis suecica (As), a species derived from A. thaliana (At) and A. arenosa (Aa) in a single event 12,000 to 300,000 years ago. We used 405,466 diagnostic features on tiling microarrays to recognize At and Aa contributions to the As genome and transcriptome: 324 genes lacked Aa contributions and 614 genes lacked At contributions within As. In leaf tissues, 3,458 genes preferentially expressed At homoeologs while 4,150 favored Aa homoeologs. These patterns were validated with resequencing. Genes with preferential use of Aa homoeologs were enriched for expression functions, consistent with the dominance of Aa transcription. Heterologous networks - mixed from At and Aa transcripts - were underrepresented.CONCLUSIONS:Thousands of deleted and silenced homoeologs in the genome of As were identified. Since heterologous networks may be compromised by interspecies incompatibilities, these networks evolve co-biases, expressing either only Aa or only At homoeologs. This progressive change towards predominantly pure parental networks might contribute to phenotypic variability and plasticity, and enable the species to exploit a larger range of environments.en
dc.language.isoenen
dc.publisherBioMed Centralen
dc.relation.urlhttp://genomebiology.com/2010/11/12/R125en
dc.rights© 2010 Chang et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0)en
dc.titleHomoeolog-specific retention and use in allotetraploid Arabidopsis suecica depends on parent of origin and network partnersen
dc.typeArticleen
dc.contributor.departmentMolecular and Computational Biology, University of Southern California, 1050 Childs Way, RRI 201, Los Angeles, CA 90089-2910, USAen
dc.contributor.departmentGenome Center and Department of Plant Biology, University of California at Davis, 451 Health Services Drive, Davis, CA 95616, USAen
dc.contributor.departmentCurrent address: Department of Horticulture and Landscape Architecture, Purdue University, 625 Agriculture Mall Drive, West Lafayette, IN 47907-2010, USAen
dc.contributor.departmentSchool of Plant Sciences, University of Arizona, 1140 E. South Campus Drive, Forbes Building, Room 303, Tucson, AZ 85721-0036, USAen
dc.identifier.journalGenome Biologyen
dc.description.collectioninformationThis item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.en
dc.eprint.versionFinal published versionen
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