Human 14-3-3 gamma protein results in abnormal cell proliferation in the developing eye of Drosophila melanogaster

Persistent Link:
http://hdl.handle.net/10150/610117
Title:
Human 14-3-3 gamma protein results in abnormal cell proliferation in the developing eye of Drosophila melanogaster
Author:
Hong, Sophia; Qi, Wenqing; Brabant, Marc; Bosco, Giovanni; Martinez, Jesse
Affiliation:
Department of Cell Biology and Anatomy, Arizona Cancer Center, University of Arizona, Tucson, Arizona 85724, USA; Department of Medicine, Arizona Cancer Center, University of Arizona, Tucson, Arizona 85724, USA; Cancer Center Division, Arizona Cancer Center, University of Arizona, Tucson, Arizona 85724, USA; Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona 85721, USA
Issue Date:
2008
Publisher:
BioMed Central
Citation:
Cell Division 2008, 3:2 doi:10.1186/1747-1028-3-2
Journal:
Cell Division
Rights:
© 2008 Hong et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0)
Collection Information:
This item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.
Abstract:
BACKGROUND:14-3-3 proteins are a family of adaptor proteins that participate in a wide variety of cellular processes. Recent evidence indicates that the expression levels of these proteins are elevated in some human tumors providing circumstantial evidence for their involvement in human cancers. However, the mechanism through which these proteins act in tumorigenesis is uncertain.RESULTS:To determine whether elevated levels of 14-3-3 proteins may perturb cell growth we overexpressed human 14-3-3 gamma (h14-3-3 gamma) in Drosophila larvae using the heat shock promoter or the GMR-Gal4 driver and then examined the effect that this had on cell proliferation in the eye imaginal discs of third instar larvae. We found that induction of h14-3-3 gamma resulted in the abnormal appearance of replicating cells in the differentiating proneural photoreceptor cells of eye imaginal discs where h14-3-3 gamma was driven by the heat shock promoter. Similarly, we found that driving h14-3-3 gamma expression specifically in developing eye discs with the GMR-Gal4 driver resulted in increased numbers of replicative cells following the morphogenetic furrow. Interestingly, we found that the effects of overexpressing h1433 gamma on eye development were increased in a genetic background where String (cdc25) function was compromised.CONCLUSION:Taken together our results indicate that h14-3-3 gamma can promote abnormal cell proliferation and may act through Cdc25. This has important implications for 14-3-3 gamma as an oncogene as it suggests that elevated levels of 14-3-3 may confer a growth advantage to cells that overexpress it.
EISSN:
1747-1028
DOI:
10.1186/1747-1028-3-2
Version:
Final published version
Additional Links:
http://www.celldiv.com/content/3/1/2

Full metadata record

DC FieldValue Language
dc.contributor.authorHong, Sophiaen
dc.contributor.authorQi, Wenqingen
dc.contributor.authorBrabant, Marcen
dc.contributor.authorBosco, Giovannien
dc.contributor.authorMartinez, Jesseen
dc.date.accessioned2016-05-20T08:58:58Z-
dc.date.available2016-05-20T08:58:58Z-
dc.date.issued2008en
dc.identifier.citationCell Division 2008, 3:2 doi:10.1186/1747-1028-3-2en
dc.identifier.doi10.1186/1747-1028-3-2en
dc.identifier.urihttp://hdl.handle.net/10150/610117-
dc.description.abstractBACKGROUND:14-3-3 proteins are a family of adaptor proteins that participate in a wide variety of cellular processes. Recent evidence indicates that the expression levels of these proteins are elevated in some human tumors providing circumstantial evidence for their involvement in human cancers. However, the mechanism through which these proteins act in tumorigenesis is uncertain.RESULTS:To determine whether elevated levels of 14-3-3 proteins may perturb cell growth we overexpressed human 14-3-3 gamma (h14-3-3 gamma) in Drosophila larvae using the heat shock promoter or the GMR-Gal4 driver and then examined the effect that this had on cell proliferation in the eye imaginal discs of third instar larvae. We found that induction of h14-3-3 gamma resulted in the abnormal appearance of replicating cells in the differentiating proneural photoreceptor cells of eye imaginal discs where h14-3-3 gamma was driven by the heat shock promoter. Similarly, we found that driving h14-3-3 gamma expression specifically in developing eye discs with the GMR-Gal4 driver resulted in increased numbers of replicative cells following the morphogenetic furrow. Interestingly, we found that the effects of overexpressing h1433 gamma on eye development were increased in a genetic background where String (cdc25) function was compromised.CONCLUSION:Taken together our results indicate that h14-3-3 gamma can promote abnormal cell proliferation and may act through Cdc25. This has important implications for 14-3-3 gamma as an oncogene as it suggests that elevated levels of 14-3-3 may confer a growth advantage to cells that overexpress it.en
dc.language.isoenen
dc.publisherBioMed Centralen
dc.relation.urlhttp://www.celldiv.com/content/3/1/2en
dc.rights© 2008 Hong et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0)en
dc.titleHuman 14-3-3 gamma protein results in abnormal cell proliferation in the developing eye of Drosophila melanogasteren
dc.typeArticleen
dc.identifier.eissn1747-1028en
dc.contributor.departmentDepartment of Cell Biology and Anatomy, Arizona Cancer Center, University of Arizona, Tucson, Arizona 85724, USAen
dc.contributor.departmentDepartment of Medicine, Arizona Cancer Center, University of Arizona, Tucson, Arizona 85724, USAen
dc.contributor.departmentCancer Center Division, Arizona Cancer Center, University of Arizona, Tucson, Arizona 85724, USAen
dc.contributor.departmentDepartment of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona 85721, USAen
dc.identifier.journalCell Divisionen
dc.description.collectioninformationThis item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.en
dc.eprint.versionFinal published versionen
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