Non-tuberculous mycobacteria isolated from slaughter pigs in Mubende district, Uganda

Persistent Link:
http://hdl.handle.net/10150/610108
Title:
Non-tuberculous mycobacteria isolated from slaughter pigs in Mubende district, Uganda
Author:
Muwonge, Adrian; Kankya, Clovice; Johansen, Tone; Djonne, Berit; Godfroid, Jacques; Biffa, Demelash; Edvardsen, Vigdis; Skjerve, Eystein
Affiliation:
Centre for Epidemiology and Biostatistics, Norwegian School of Veterinary Science, P.O. Box 8146 Dep, 0033, Oslo, Norway; Department of Biosecurity, Ecosystems and Public Health (BEP), College Of Veterinary Medicine, Animal Resources & Biosecurity Makerere University, P.O. Box 7062, Kampala, Uganda; Norwegian Veterinary Institute, P.O. Box 750, N-0106, Oslo, Norway; Section for Arctic Veterinary Medicine, Norwegian School of Veterinary Science, Stakkevollveien 9010, Tromsø, Norway; College of Medicine, University of Arizona, 1656 E. Mabel St, P.O. Box 245221, Tucson, AZ, 85724, USA
Issue Date:
2012
Publisher:
BioMed Central
Citation:
Muwonge et al. BMC Veterinary Research 2012, 8:52 http://www.biomedcentral.com/1746-6148/8/52
Journal:
BMC Veterinary Research
Rights:
© 2012 Muwonge et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0)
Collection Information:
This item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.
Abstract:
BACKGROUND:The importance of infections caused by non-tuberculous mycobacteria (NTM) in animals and humans has gained considerable recognition during the past few years. In the developed world, where pig production is extensively practiced, studies on mycobacterial infections and related control strategies have received increasing attention. The infections are reported to be caused by a wide spectrum of NTM. Unfortunately, these infections have been less recognized in sub-Saharan Africa owing to lack of awareness and systematic studies. In this study we aimed at isolating and identifying species of mycobacteria involved in causing infections in slaughter pigs in Mubende district of Uganda. Furthermore we wanted to identify factors associated with infection prevalence in the study area.METHODS:A total of 363 lymph nodes were collected and cultured for the presence of mycobacteria. Isolates were identified by 16S rDNA gene sequencing. A questionnaire survey was administered to identify production related factors associated with infection prevalence. Data were assembled and analysed using descriptive statistics and mixed effects logistic regression analysis.RESULTS:Mycobacteria were detected in 39 % (143/363) of the examined lymph nodes, 63 % (59/93) of lymph nodes with gross lesions typical of mycobacteriosis and 31% (84/270) of lymph nodes with no visible lesions. Nineteen per cent of the isolated mycobacteria were identified as Mycobacterium (M) avium, of these 78% and 22% were M. avium sub sp. Hominissuis and avium respectively. Other mycobacterial species included M. senuense (16%), M. terrae (7%) and M. asiaticum (6%). This study found free range systems (OR=3.0; P=0.034) and use of water from valley dams (OR=2.0; P=0.049) as factors associated with high prevalence of mycobacteria in slaughter pigs.CONCLUSIONS:This study demonstrated a high prevalence of NTM infections among slaughter pigs in Mubende district of Uganda. M. avium was the most prevalent of all NTM isolated and identified. Free range system of pig management and valley dam water were the most significant factors associated with NTM prevalence in Mubende district. These findings could be of a major public health concern given that it is in a predominantly pork consuming population with 18% HIV/AIDS prevalence. Therefore, stringent post-mortem inspection at the slaughter houses is of paramount importance to reduce human exposure.
EISSN:
1746-6148
DOI:
10.1186/1746-6148-8-52
Version:
Final published version
Additional Links:
http://www.biomedcentral.com/1746-6148/8/52

Full metadata record

DC FieldValue Language
dc.contributor.authorMuwonge, Adrianen
dc.contributor.authorKankya, Cloviceen
dc.contributor.authorJohansen, Toneen
dc.contributor.authorDjonne, Beriten
dc.contributor.authorGodfroid, Jacquesen
dc.contributor.authorBiffa, Demelashen
dc.contributor.authorEdvardsen, Vigdisen
dc.contributor.authorSkjerve, Eysteinen
dc.date.accessioned2016-05-20T08:58:46Z-
dc.date.available2016-05-20T08:58:46Z-
dc.date.issued2012en
dc.identifier.citationMuwonge et al. BMC Veterinary Research 2012, 8:52 http://www.biomedcentral.com/1746-6148/8/52en
dc.identifier.doi10.1186/1746-6148-8-52en
dc.identifier.urihttp://hdl.handle.net/10150/610108-
dc.description.abstractBACKGROUND:The importance of infections caused by non-tuberculous mycobacteria (NTM) in animals and humans has gained considerable recognition during the past few years. In the developed world, where pig production is extensively practiced, studies on mycobacterial infections and related control strategies have received increasing attention. The infections are reported to be caused by a wide spectrum of NTM. Unfortunately, these infections have been less recognized in sub-Saharan Africa owing to lack of awareness and systematic studies. In this study we aimed at isolating and identifying species of mycobacteria involved in causing infections in slaughter pigs in Mubende district of Uganda. Furthermore we wanted to identify factors associated with infection prevalence in the study area.METHODS:A total of 363 lymph nodes were collected and cultured for the presence of mycobacteria. Isolates were identified by 16S rDNA gene sequencing. A questionnaire survey was administered to identify production related factors associated with infection prevalence. Data were assembled and analysed using descriptive statistics and mixed effects logistic regression analysis.RESULTS:Mycobacteria were detected in 39 % (143/363) of the examined lymph nodes, 63 % (59/93) of lymph nodes with gross lesions typical of mycobacteriosis and 31% (84/270) of lymph nodes with no visible lesions. Nineteen per cent of the isolated mycobacteria were identified as Mycobacterium (M) avium, of these 78% and 22% were M. avium sub sp. Hominissuis and avium respectively. Other mycobacterial species included M. senuense (16%), M. terrae (7%) and M. asiaticum (6%). This study found free range systems (OR=3.0en
dc.description.abstractP=0.034) and use of water from valley dams (OR=2.0en
dc.description.abstractP=0.049) as factors associated with high prevalence of mycobacteria in slaughter pigs.CONCLUSIONS:This study demonstrated a high prevalence of NTM infections among slaughter pigs in Mubende district of Uganda. M. avium was the most prevalent of all NTM isolated and identified. Free range system of pig management and valley dam water were the most significant factors associated with NTM prevalence in Mubende district. These findings could be of a major public health concern given that it is in a predominantly pork consuming population with 18% HIV/AIDS prevalence. Therefore, stringent post-mortem inspection at the slaughter houses is of paramount importance to reduce human exposure.en
dc.language.isoenen
dc.publisherBioMed Centralen
dc.relation.urlhttp://www.biomedcentral.com/1746-6148/8/52en
dc.rights© 2012 Muwonge et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0)en
dc.titleNon-tuberculous mycobacteria isolated from slaughter pigs in Mubende district, Ugandaen
dc.typeArticleen
dc.identifier.eissn1746-6148en
dc.contributor.departmentCentre for Epidemiology and Biostatistics, Norwegian School of Veterinary Science, P.O. Box 8146 Dep, 0033, Oslo, Norwayen
dc.contributor.departmentDepartment of Biosecurity, Ecosystems and Public Health (BEP), College Of Veterinary Medicine, Animal Resources & Biosecurity Makerere University, P.O. Box 7062, Kampala, Ugandaen
dc.contributor.departmentNorwegian Veterinary Institute, P.O. Box 750, N-0106, Oslo, Norwayen
dc.contributor.departmentSection for Arctic Veterinary Medicine, Norwegian School of Veterinary Science, Stakkevollveien 9010, Tromsø, Norwayen
dc.contributor.departmentCollege of Medicine, University of Arizona, 1656 E. Mabel St, P.O. Box 245221, Tucson, AZ, 85724, USAen
dc.identifier.journalBMC Veterinary Researchen
dc.description.collectioninformationThis item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.en
dc.eprint.versionFinal published versionen
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