Mycobacterium bovis infections in slaughter pigs in Mubende district, Uganda: a public health concern

Persistent Link:
http://hdl.handle.net/10150/610107
Title:
Mycobacterium bovis infections in slaughter pigs in Mubende district, Uganda: a public health concern
Author:
Muwonge, Adrian; Johansen, Tone; Vigdis, Edvardsen; Godfroid, Jacques; Olea-Popelka, Francisco; Biffa, Demelash; Skjerve, Eystein; Djonne, Berit
Affiliation:
Department of Food Safety and Infection Biology, Norwegian School of Veterinary Science, P.O. Box 8146, Dep., 0033, Oslo, Norway; Norwegian Veterinary Institute, P.O. Box 750, N-0106, Oslo, Norway; Department of Food Safety and Infection Biology, Section of Arctic Veterinary Medicine, Norwegian School of Veterinary Science, Stakkevollveien 23, 9010, Tromsø, Norway; Department of Clinical Sciences College of Veterinary Medicine and Biomedical Sciences Colorado State University Fort Collins, Fort Collins, CO, 80523-1678, USA; College of Medicine, University of Arizona, 1656 E. Mabel St, P.O. Box 245221, Tucson, AZ, 85724, USA
Issue Date:
2012
Publisher:
BioMed Central
Citation:
Muwonge et al. BMC Veterinary Research 2012, 8:168 http://www.biomedcentral.com/1746-6148/8/168
Journal:
BMC Veterinary Research
Rights:
© 2012 Muwonge et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0)
Collection Information:
This item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.
Abstract:
BACKGROUND:Bovine tuberculosis (TB) caused by Mycobacterium bovis is primarily a disease of ruminants, particularly cattle (Bos primigenius) and buffalo (Syncerus caffer), and is endemic in most developing countries. To date, studies done in Uganda have documented the prevalence of M. bovis in cattle, humans and wild life, in addition to non-tuberculous mycobacteria in pigs. Pigs are increasingly becoming an important component of the livestock sector and share the human ecosystem in rural Uganda. It is therefore of public health interest that they are not a source of human infections. As a follow up to previously published findings on mycobacteria in pigs, this study was aimed at investigating the occurrence and molecular characteristics of M. bovis detected in slaughter pigs in Mubende district, Uganda. One hundred fifty mesenteric lymph nodes with lesions suggestive of mycobacterial infections were collected from approximately one thousand slaughtered pigs in Mubende district over a period of five months. The isolation and identification of M. bovis was done using conventional mycobacteriological methods. Mycobacteria belonging to the Mycobacterium tuberculosis complex (MTC) were identified to species level using deletion analysis. Molecular typing was done using Spoligotyping and MIRU-VNTR analysis. Molecular data were analysed and interpreted using MIRU-VNTR plus, SpolDB4.0 and the Mycobacterium bovis spoligo database.RESULTS:Of the examined animals, one boar and two sows from Madudu Sub County were infected with M. bovis which presented as lesions of a deep yellow colour and a grit-like texture in the mesenteric lymph nodes. This represents 2% (3/150) of the lymph nodes where lesions suggestive of mycobacterial infections were detected. Molecular analysis revealed that the isolates from the infected pigs showed identical MIRU-VNTR profile and spoligotype (SB1469).CONCLUSIONS:This is the first study documenting the occurrence of M. bovis in slaughter pigs in Uganda, revealing that one in fifty slaughter pigs with suspected lesions in mesenteric lymph nodes were infected. Molecular analysis revealed that the isolates were identical, showing a spoligotype previously reported from humans and cattle in the north eastern part of the Uganda cattle corridor. This finding is of public health importance, therefore there is a need for close cooperation between medical and veterinary professionals in designing and implementing control and prevention measures that safeguard the public from this potential source of zoonotic TB in Uganda.
EISSN:
1746-6148
DOI:
10.1186/1746-6148-8-168
Keywords:
Pigs; Spoligotype; MIRU-VNTR; M. bovis; Uganda
Version:
Final published version
Additional Links:
http://www.biomedcentral.com/1746-6148/8/168

Full metadata record

DC FieldValue Language
dc.contributor.authorMuwonge, Adrianen
dc.contributor.authorJohansen, Toneen
dc.contributor.authorVigdis, Edvardsenen
dc.contributor.authorGodfroid, Jacquesen
dc.contributor.authorOlea-Popelka, Franciscoen
dc.contributor.authorBiffa, Demelashen
dc.contributor.authorSkjerve, Eysteinen
dc.contributor.authorDjonne, Beriten
dc.date.accessioned2016-05-20T08:58:44Z-
dc.date.available2016-05-20T08:58:44Z-
dc.date.issued2012en
dc.identifier.citationMuwonge et al. BMC Veterinary Research 2012, 8:168 http://www.biomedcentral.com/1746-6148/8/168en
dc.identifier.doi10.1186/1746-6148-8-168en
dc.identifier.urihttp://hdl.handle.net/10150/610107-
dc.description.abstractBACKGROUND:Bovine tuberculosis (TB) caused by Mycobacterium bovis is primarily a disease of ruminants, particularly cattle (Bos primigenius) and buffalo (Syncerus caffer), and is endemic in most developing countries. To date, studies done in Uganda have documented the prevalence of M. bovis in cattle, humans and wild life, in addition to non-tuberculous mycobacteria in pigs. Pigs are increasingly becoming an important component of the livestock sector and share the human ecosystem in rural Uganda. It is therefore of public health interest that they are not a source of human infections. As a follow up to previously published findings on mycobacteria in pigs, this study was aimed at investigating the occurrence and molecular characteristics of M. bovis detected in slaughter pigs in Mubende district, Uganda. One hundred fifty mesenteric lymph nodes with lesions suggestive of mycobacterial infections were collected from approximately one thousand slaughtered pigs in Mubende district over a period of five months. The isolation and identification of M. bovis was done using conventional mycobacteriological methods. Mycobacteria belonging to the Mycobacterium tuberculosis complex (MTC) were identified to species level using deletion analysis. Molecular typing was done using Spoligotyping and MIRU-VNTR analysis. Molecular data were analysed and interpreted using MIRU-VNTR plus, SpolDB4.0 and the Mycobacterium bovis spoligo database.RESULTS:Of the examined animals, one boar and two sows from Madudu Sub County were infected with M. bovis which presented as lesions of a deep yellow colour and a grit-like texture in the mesenteric lymph nodes. This represents 2% (3/150) of the lymph nodes where lesions suggestive of mycobacterial infections were detected. Molecular analysis revealed that the isolates from the infected pigs showed identical MIRU-VNTR profile and spoligotype (SB1469).CONCLUSIONS:This is the first study documenting the occurrence of M. bovis in slaughter pigs in Uganda, revealing that one in fifty slaughter pigs with suspected lesions in mesenteric lymph nodes were infected. Molecular analysis revealed that the isolates were identical, showing a spoligotype previously reported from humans and cattle in the north eastern part of the Uganda cattle corridor. This finding is of public health importance, therefore there is a need for close cooperation between medical and veterinary professionals in designing and implementing control and prevention measures that safeguard the public from this potential source of zoonotic TB in Uganda.en
dc.language.isoenen
dc.publisherBioMed Centralen
dc.relation.urlhttp://www.biomedcentral.com/1746-6148/8/168en
dc.rights© 2012 Muwonge et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0)en
dc.subjectPigsen
dc.subjectSpoligotypeen
dc.subjectMIRU-VNTRen
dc.subjectM. bovisen
dc.subjectUgandaen
dc.titleMycobacterium bovis infections in slaughter pigs in Mubende district, Uganda: a public health concernen
dc.typeArticleen
dc.identifier.eissn1746-6148en
dc.contributor.departmentDepartment of Food Safety and Infection Biology, Norwegian School of Veterinary Science, P.O. Box 8146, Dep., 0033, Oslo, Norwayen
dc.contributor.departmentNorwegian Veterinary Institute, P.O. Box 750, N-0106, Oslo, Norwayen
dc.contributor.departmentDepartment of Food Safety and Infection Biology, Section of Arctic Veterinary Medicine, Norwegian School of Veterinary Science, Stakkevollveien 23, 9010, Tromsø, Norwayen
dc.contributor.departmentDepartment of Clinical Sciences College of Veterinary Medicine and Biomedical Sciences Colorado State University Fort Collins, Fort Collins, CO, 80523-1678, USAen
dc.contributor.departmentCollege of Medicine, University of Arizona, 1656 E. Mabel St, P.O. Box 245221, Tucson, AZ, 85724, USAen
dc.identifier.journalBMC Veterinary Researchen
dc.description.collectioninformationThis item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.en
dc.eprint.versionFinal published versionen
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