Gene expression analyses in maize inbreds and hybrids with varying levels of heterosis

Persistent Link:
http://hdl.handle.net/10150/610080
Title:
Gene expression analyses in maize inbreds and hybrids with varying levels of heterosis
Author:
Stupar, Robert; Gardiner, Jack; Oldre, Aaron; Haun, William; Chandler, Vicki; Springer, Nathan
Affiliation:
Center for Plant and Microbial Genomics, Department of Plant Biology, University of Minnesota, Saint Paul MN 55108, USA; Department of Plant Science, and BIO5 Institute, University of Arizona, Tucson, AZ 85721, USA; Department of Agronomy and Plant Genetics, University of Minnesota, Saint Paul MN 55108, USA
Issue Date:
2008
Publisher:
BioMed Central
Citation:
BMC Plant Biology 2008, 8:33 doi:10.1186/1471-2229-8-33
Journal:
BMC Plant Biology
Rights:
© 2008 Stupar et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0)
Collection Information:
This item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.
Abstract:
BACKGROUND:Heterosis is the superior performance of F1 hybrid progeny relative to the parental phenotypes. Maize exhibits heterosis for a wide range of traits, however the magnitude of heterosis is highly variable depending on the choice of parents and the trait(s) measured. We have used expression profiling to determine whether the level, or types, of non-additive gene expression vary in maize hybrids with different levels of genetic diversity or heterosis.RESULTS:We observed that the distributions of better parent heterosis among a series of 25 maize hybrids generally do not exhibit significant correlations between different traits. Expression profiling analyses for six of these hybrids, chosen to represent diversity in genotypes and heterosis responses, revealed a correlation between genetic diversity and transcriptional variation. The majority of differentially expressed genes in each of the six different hybrids exhibited additive expression patterns, and ~25% exhibited statistically significant non-additive expression profiles. Among the non-additive profiles, ~80% exhibited hybrid expression levels between the parental levels, ~20% exhibited hybrid expression levels at the parental levels and ~1% exhibited hybrid levels outside the parental range.CONCLUSION:We have found that maize inbred genetic diversity is correlated with transcriptional variation. However, sampling of seedling tissues indicated that the frequencies of additive and non-additive expression patterns are very similar across a range of hybrid lines. These findings suggest that heterosis is probably not a consequence of higher levels of additive or non-additive expression, but may be related to transcriptional variation between parents. The lack of correlation between better parent heterosis levels for different traits suggests that transcriptional diversity at specific sets of genes may influence heterosis for different traits.
EISSN:
1471-2229
DOI:
10.1186/1471-2229-8-33
Version:
Final published version
Additional Links:
http://www.biomedcentral.com/1471-2229/8/33

Full metadata record

DC FieldValue Language
dc.contributor.authorStupar, Roberten
dc.contributor.authorGardiner, Jacken
dc.contributor.authorOldre, Aaronen
dc.contributor.authorHaun, Williamen
dc.contributor.authorChandler, Vickien
dc.contributor.authorSpringer, Nathanen
dc.date.accessioned2016-05-20T08:58:06Z-
dc.date.available2016-05-20T08:58:06Z-
dc.date.issued2008en
dc.identifier.citationBMC Plant Biology 2008, 8:33 doi:10.1186/1471-2229-8-33en
dc.identifier.doi10.1186/1471-2229-8-33en
dc.identifier.urihttp://hdl.handle.net/10150/610080-
dc.description.abstractBACKGROUND:Heterosis is the superior performance of F1 hybrid progeny relative to the parental phenotypes. Maize exhibits heterosis for a wide range of traits, however the magnitude of heterosis is highly variable depending on the choice of parents and the trait(s) measured. We have used expression profiling to determine whether the level, or types, of non-additive gene expression vary in maize hybrids with different levels of genetic diversity or heterosis.RESULTS:We observed that the distributions of better parent heterosis among a series of 25 maize hybrids generally do not exhibit significant correlations between different traits. Expression profiling analyses for six of these hybrids, chosen to represent diversity in genotypes and heterosis responses, revealed a correlation between genetic diversity and transcriptional variation. The majority of differentially expressed genes in each of the six different hybrids exhibited additive expression patterns, and ~25% exhibited statistically significant non-additive expression profiles. Among the non-additive profiles, ~80% exhibited hybrid expression levels between the parental levels, ~20% exhibited hybrid expression levels at the parental levels and ~1% exhibited hybrid levels outside the parental range.CONCLUSION:We have found that maize inbred genetic diversity is correlated with transcriptional variation. However, sampling of seedling tissues indicated that the frequencies of additive and non-additive expression patterns are very similar across a range of hybrid lines. These findings suggest that heterosis is probably not a consequence of higher levels of additive or non-additive expression, but may be related to transcriptional variation between parents. The lack of correlation between better parent heterosis levels for different traits suggests that transcriptional diversity at specific sets of genes may influence heterosis for different traits.en
dc.language.isoenen
dc.publisherBioMed Centralen
dc.relation.urlhttp://www.biomedcentral.com/1471-2229/8/33en
dc.rights© 2008 Stupar et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0)en
dc.titleGene expression analyses in maize inbreds and hybrids with varying levels of heterosisen
dc.typeArticleen
dc.identifier.eissn1471-2229en
dc.contributor.departmentCenter for Plant and Microbial Genomics, Department of Plant Biology, University of Minnesota, Saint Paul MN 55108, USAen
dc.contributor.departmentDepartment of Plant Science, and BIO5 Institute, University of Arizona, Tucson, AZ 85721, USAen
dc.contributor.departmentDepartment of Agronomy and Plant Genetics, University of Minnesota, Saint Paul MN 55108, USAen
dc.identifier.journalBMC Plant Biologyen
dc.description.collectioninformationThis item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.en
dc.eprint.versionFinal published versionen
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