Manipulating insulin signaling to enhance mosquito reproduction

Persistent Link:
http://hdl.handle.net/10150/610077
Title:
Manipulating insulin signaling to enhance mosquito reproduction
Author:
Arik, Anam; Rasgon, Jason; Quicke, Kendra; Riehle, Michael
Affiliation:
Department of Entomology – University of Arizona, Tucson, AZ, USA; The W. Harry Feinstone Department of Molecular Microbiology and Immunology – Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, USA; The Johns Hopkins Malaria Research Institute – Johns Hopkins University, Baltimore, MD, USA
Issue Date:
2009
Publisher:
BioMed Central
Citation:
BMC Physiology 2009, 9:15 doi:10.1186/1472-6793-9-15
Journal:
BMC Physiology
Rights:
© 2009 Arik et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0)
Collection Information:
This item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.
Abstract:
BACKGROND:In the mosquito Aedes aegypti the insulin/insulin growth factor I signaling (IIS) cascade is a key regulator of many physiological processes, including reproduction. Two important reproductive events, steroidogenesis in the ovary and yolk synthesis in the fat body, are regulated by the IIS cascade in mosquitoes. The signaling molecule phosphatase and tensin homolog (PTEN) is a key inhibitor of the IIS cascade that helps modulate the activity of the IIS cascade. In Ae. aegypti, six unique splice variants of AaegPTEN were previously identified, but the role of these splice variants, particularly AaegPTEN3 and 6, were unknown.RESULTS:Knockdown of AaegPTEN or its specific splice variant AaegPTEN6 (the splice variant thought to regulate reproduction in the ovary and fat body) using RNAi led to a 15-63% increase in egg production with no adverse effects on egg viability during the first reproductive cycle. Knockdown of AaegPTEN3, expressed predominantly in the head, had no effect on reproduction. We also characterized the protein expression patterns of these two splice variants during development and in various tissues during a reproductive cycle.CONCLUSION:Previous studies in a range of organisms, including Drosophila melanogaster and Caenorhabditis elegans, have demonstrated that disruption of the IIS cascade leads to decreased reproduction or sterility. In this study we demonstrate that knockdown of the IIS inhibitor PTEN can actually increase reproduction in the mosquito, at least during the first reproductive cycle.
EISSN:
1472-6793
DOI:
10.1186/1472-6793-9-15
Version:
Final published version
Additional Links:
http://www.biomedcentral.com/1472-6793/9/15

Full metadata record

DC FieldValue Language
dc.contributor.authorArik, Anamen
dc.contributor.authorRasgon, Jasonen
dc.contributor.authorQuicke, Kendraen
dc.contributor.authorRiehle, Michaelen
dc.date.accessioned2016-05-20T08:58:02Z-
dc.date.available2016-05-20T08:58:02Z-
dc.date.issued2009en
dc.identifier.citationBMC Physiology 2009, 9:15 doi:10.1186/1472-6793-9-15en
dc.identifier.doi10.1186/1472-6793-9-15en
dc.identifier.urihttp://hdl.handle.net/10150/610077-
dc.description.abstractBACKGROND:In the mosquito Aedes aegypti the insulin/insulin growth factor I signaling (IIS) cascade is a key regulator of many physiological processes, including reproduction. Two important reproductive events, steroidogenesis in the ovary and yolk synthesis in the fat body, are regulated by the IIS cascade in mosquitoes. The signaling molecule phosphatase and tensin homolog (PTEN) is a key inhibitor of the IIS cascade that helps modulate the activity of the IIS cascade. In Ae. aegypti, six unique splice variants of AaegPTEN were previously identified, but the role of these splice variants, particularly AaegPTEN3 and 6, were unknown.RESULTS:Knockdown of AaegPTEN or its specific splice variant AaegPTEN6 (the splice variant thought to regulate reproduction in the ovary and fat body) using RNAi led to a 15-63% increase in egg production with no adverse effects on egg viability during the first reproductive cycle. Knockdown of AaegPTEN3, expressed predominantly in the head, had no effect on reproduction. We also characterized the protein expression patterns of these two splice variants during development and in various tissues during a reproductive cycle.CONCLUSION:Previous studies in a range of organisms, including Drosophila melanogaster and Caenorhabditis elegans, have demonstrated that disruption of the IIS cascade leads to decreased reproduction or sterility. In this study we demonstrate that knockdown of the IIS inhibitor PTEN can actually increase reproduction in the mosquito, at least during the first reproductive cycle.en
dc.language.isoenen
dc.publisherBioMed Centralen
dc.relation.urlhttp://www.biomedcentral.com/1472-6793/9/15en
dc.rights© 2009 Arik et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0)en
dc.titleManipulating insulin signaling to enhance mosquito reproductionen
dc.typeArticleen
dc.identifier.eissn1472-6793en
dc.contributor.departmentDepartment of Entomology – University of Arizona, Tucson, AZ, USAen
dc.contributor.departmentThe W. Harry Feinstone Department of Molecular Microbiology and Immunology – Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, USAen
dc.contributor.departmentThe Johns Hopkins Malaria Research Institute – Johns Hopkins University, Baltimore, MD, USAen
dc.identifier.journalBMC Physiologyen
dc.description.collectioninformationThis item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.en
dc.eprint.versionFinal published versionen
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