Expression of epidermal growth factor, transforming growth factor-beta1 and adiponectin in nipple aspirate fluid and plasma of pre and post-menopausal women

Persistent Link:
http://hdl.handle.net/10150/610054
Title:
Expression of epidermal growth factor, transforming growth factor-beta1 and adiponectin in nipple aspirate fluid and plasma of pre and post-menopausal women
Author:
Miller, Jessica; Thompson, Patricia; Hakim, Iman; Lopez, Ana Maria; Thomson, Cynthia; Hsu, Chiu-Hsieh; Chow, H-H S.
Affiliation:
The University of Arizona Cancer Center, Tucson, AZ, USA; Zuckerman College of Public Health, The University of Arizona, Tucson, AZ, USA
Issue Date:
2013
Publisher:
BioMed Central
Citation:
Miller et al. Biomarker Research 2013, 1:18 http://www.biomarkerres.org/content/1/1/18
Journal:
Biomarker Research
Rights:
© 2013 Miller et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0)
Collection Information:
This item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.
Abstract:
BACKGROUND:Nipple aspirate fluid (NAF) contains large amounts of protein thought to reflect the microenvironment of the breast, and is of interest in breast cancer prevention research. The correlation between specific NAF proteins to plasma concentrations have not been well studied in healthy women. We collected matched NAF and plasma from 43 healthy pre and postmenopausal women participating in an early phase clinical study to compare the levels of putative cancer protein biomarkers. We compared baseline NAF and plasma levels of epidermal growth factor (EGF), transforming growth factor-beta 1 (TGF-beta1), and adiponectin and evaluated menopausal status and body mass index (BMI) as potential modifying factors.FINDINGS:NAF and plasma levels of EGF, TGF-beta1 and adiponectin were not correlated. EGF and TGF-beta1 levels in NAF of premenopausal women were significantly higher than postmenopausal women (P's<0.01). These differences by menopausal status were not observed in plasma. Both NAF and plasma adiponectin levels were non-significantly higher in postmenopausal women. NAF biomarker levels were not associated with BMI whereas plasma EGF, TGF-beta1 and adiponectin levels in postmenopausal women were all inversely correlated with BMI (P's<0.05).CONCLUSIONS:Protein biomarkers differ significantly between NAF and plasma and are affected differently by both BMI and menopausal status. This study demonstrates important differences in biological information gained by characterizing biomarkers in NAF compared to plasma and suggests each sample source may independently inform on breast cancer risk.
EISSN:
2050-7771
DOI:
10.1186/2050-7771-1-18
Version:
Final published version
Additional Links:
http://www.biomarkerres.org/content/1/1/18

Full metadata record

DC FieldValue Language
dc.contributor.authorMiller, Jessicaen
dc.contributor.authorThompson, Patriciaen
dc.contributor.authorHakim, Imanen
dc.contributor.authorLopez, Ana Mariaen
dc.contributor.authorThomson, Cynthiaen
dc.contributor.authorHsu, Chiu-Hsiehen
dc.contributor.authorChow, H-H S.en
dc.date.accessioned2016-05-20T08:57:31Z-
dc.date.available2016-05-20T08:57:31Z-
dc.date.issued2013en
dc.identifier.citationMiller et al. Biomarker Research 2013, 1:18 http://www.biomarkerres.org/content/1/1/18en
dc.identifier.doi10.1186/2050-7771-1-18en
dc.identifier.urihttp://hdl.handle.net/10150/610054-
dc.description.abstractBACKGROUND:Nipple aspirate fluid (NAF) contains large amounts of protein thought to reflect the microenvironment of the breast, and is of interest in breast cancer prevention research. The correlation between specific NAF proteins to plasma concentrations have not been well studied in healthy women. We collected matched NAF and plasma from 43 healthy pre and postmenopausal women participating in an early phase clinical study to compare the levels of putative cancer protein biomarkers. We compared baseline NAF and plasma levels of epidermal growth factor (EGF), transforming growth factor-beta 1 (TGF-beta1), and adiponectin and evaluated menopausal status and body mass index (BMI) as potential modifying factors.FINDINGS:NAF and plasma levels of EGF, TGF-beta1 and adiponectin were not correlated. EGF and TGF-beta1 levels in NAF of premenopausal women were significantly higher than postmenopausal women (P's<0.01). These differences by menopausal status were not observed in plasma. Both NAF and plasma adiponectin levels were non-significantly higher in postmenopausal women. NAF biomarker levels were not associated with BMI whereas plasma EGF, TGF-beta1 and adiponectin levels in postmenopausal women were all inversely correlated with BMI (P's<0.05).CONCLUSIONS:Protein biomarkers differ significantly between NAF and plasma and are affected differently by both BMI and menopausal status. This study demonstrates important differences in biological information gained by characterizing biomarkers in NAF compared to plasma and suggests each sample source may independently inform on breast cancer risk.en
dc.language.isoenen
dc.publisherBioMed Centralen
dc.relation.urlhttp://www.biomarkerres.org/content/1/1/18en
dc.rights© 2013 Miller et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0)en
dc.titleExpression of epidermal growth factor, transforming growth factor-beta1 and adiponectin in nipple aspirate fluid and plasma of pre and post-menopausal womenen
dc.typeArticleen
dc.identifier.eissn2050-7771en
dc.contributor.departmentThe University of Arizona Cancer Center, Tucson, AZ, USAen
dc.contributor.departmentZuckerman College of Public Health, The University of Arizona, Tucson, AZ, USAen
dc.identifier.journalBiomarker Researchen
dc.description.collectioninformationThis item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.en
dc.eprint.versionFinal published versionen
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