Arsenophonus, an emerging clade of intracellular symbionts with a broad host distribution

Persistent Link:
http://hdl.handle.net/10150/610050
Title:
Arsenophonus, an emerging clade of intracellular symbionts with a broad host distribution
Author:
Novakova, Eva; Hypsa, Vaclav; Moran, Nancy
Affiliation:
Faculty of Science, University of South Bohemia, Branišovská 31, České Budějovice 37005, Czech Republic; Faculty of Science, University of South Bohemia and Institute of Parasitology, Biology Centre of ASCR, Branišovská 31, České Budějovice 37005, Czech Republic; Department of Ecology and Evolutionary Biology, The University of Arizona, 1041 E. Lowell St, Tucson, Arizona 85721-0088, USA
Issue Date:
2009
Publisher:
BioMed Central
Citation:
BMC Microbiology 2009, 9:143 doi:10.1186/1471-2180-9-143
Journal:
BMC Microbiology
Rights:
© 2009 Nováková et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0)
Collection Information:
This item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.
Abstract:
BACKGROUND:The genus Arsenophonus is a group of symbiotic, mainly insect-associated bacteria with rapidly increasing number of records. It is known from a broad spectrum of hosts and symbiotic relationships varying from parasitic son-killers to coevolving mutualists.The present study extends the currently known diversity with 34 samples retrieved mainly from hippoboscid (Diptera: Hippoboscidae) and nycteribiid (Diptera: Nycteribiidae) hosts, and investigates phylogenetic relationships within the genus.RESULTS:The analysis of 110 Arsenophonus sequences (incl. Riesia and Phlomobacter), provides a robust monophyletic clade, characterized by unique molecular synapomorphies. On the other hand, unstable inner topology indicates that complete understanding of Arsenophonus evolution cannot be achieved with 16S rDNA. Moreover, taxonomically restricted Sampling matrices prove sensitivity of the phylogenetic signal to sampling; in some cases, Arsenophonus monophyly is disrupted by other symbiotic bacteria. Two contrasting coevolutionary patterns occur throughout the tree: parallel host-symbiont evolution and the haphazard association of the symbionts with distant hosts. A further conspicuous feature of the topology is the occurrence of monophyletic symbiont lineages associated with monophyletic groups of hosts without a co-speciation pattern. We suggest that part of this incongruence could be caused by methodological artifacts, such as intragenomic variability.CONCLUSION:The sample of currently available molecular data presents the genus Arsenophonus as one of the richest and most widespread clusters of insect symbiotic bacteria. The analysis of its phylogenetic lineages indicates a complex evolution and apparent ecological versatility with switches between entirely different life styles. Due to these properties, the genus should play an important role in the studies of evolutionary trends in insect intracellular symbionts. However, under the current practice, relying exclusively on 16S rRNA sequences, the phylogenetic analyses are sensitive to various methodological artifacts that may even lead to description of new Arsenophonus lineages as independent genera (e.g. Riesia and Phlomobacter). The resolution of the evolutionary questions encountered within the Arsenophonus clade will thus require identification of new molecular markers suitable for the low-level phylogenetics.
EISSN:
1471-2180
DOI:
10.1186/1471-2180-9-143
Version:
Final published version
Additional Links:
http://www.biomedcentral.com/1471-2180/9/143

Full metadata record

DC FieldValue Language
dc.contributor.authorNovakova, Evaen
dc.contributor.authorHypsa, Vaclaven
dc.contributor.authorMoran, Nancyen
dc.date.accessioned2016-05-20T08:57:26Z-
dc.date.available2016-05-20T08:57:26Z-
dc.date.issued2009en
dc.identifier.citationBMC Microbiology 2009, 9:143 doi:10.1186/1471-2180-9-143en
dc.identifier.doi10.1186/1471-2180-9-143en
dc.identifier.urihttp://hdl.handle.net/10150/610050-
dc.description.abstractBACKGROUND:The genus Arsenophonus is a group of symbiotic, mainly insect-associated bacteria with rapidly increasing number of records. It is known from a broad spectrum of hosts and symbiotic relationships varying from parasitic son-killers to coevolving mutualists.The present study extends the currently known diversity with 34 samples retrieved mainly from hippoboscid (Diptera: Hippoboscidae) and nycteribiid (Diptera: Nycteribiidae) hosts, and investigates phylogenetic relationships within the genus.RESULTS:The analysis of 110 Arsenophonus sequences (incl. Riesia and Phlomobacter), provides a robust monophyletic clade, characterized by unique molecular synapomorphies. On the other hand, unstable inner topology indicates that complete understanding of Arsenophonus evolution cannot be achieved with 16S rDNA. Moreover, taxonomically restricted Sampling matrices prove sensitivity of the phylogenetic signal to samplingen
dc.description.abstractin some cases, Arsenophonus monophyly is disrupted by other symbiotic bacteria. Two contrasting coevolutionary patterns occur throughout the tree: parallel host-symbiont evolution and the haphazard association of the symbionts with distant hosts. A further conspicuous feature of the topology is the occurrence of monophyletic symbiont lineages associated with monophyletic groups of hosts without a co-speciation pattern. We suggest that part of this incongruence could be caused by methodological artifacts, such as intragenomic variability.CONCLUSION:The sample of currently available molecular data presents the genus Arsenophonus as one of the richest and most widespread clusters of insect symbiotic bacteria. The analysis of its phylogenetic lineages indicates a complex evolution and apparent ecological versatility with switches between entirely different life styles. Due to these properties, the genus should play an important role in the studies of evolutionary trends in insect intracellular symbionts. However, under the current practice, relying exclusively on 16S rRNA sequences, the phylogenetic analyses are sensitive to various methodological artifacts that may even lead to description of new Arsenophonus lineages as independent genera (e.g. Riesia and Phlomobacter). The resolution of the evolutionary questions encountered within the Arsenophonus clade will thus require identification of new molecular markers suitable for the low-level phylogenetics.en
dc.language.isoenen
dc.publisherBioMed Centralen
dc.relation.urlhttp://www.biomedcentral.com/1471-2180/9/143en
dc.rights© 2009 Nováková et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0)en
dc.titleArsenophonus, an emerging clade of intracellular symbionts with a broad host distributionen
dc.typeArticleen
dc.identifier.eissn1471-2180en
dc.contributor.departmentFaculty of Science, University of South Bohemia, Branišovská 31, České Budějovice 37005, Czech Republicen
dc.contributor.departmentFaculty of Science, University of South Bohemia and Institute of Parasitology, Biology Centre of ASCR, Branišovská 31, České Budějovice 37005, Czech Republicen
dc.contributor.departmentDepartment of Ecology and Evolutionary Biology, The University of Arizona, 1041 E. Lowell St, Tucson, Arizona 85721-0088, USAen
dc.identifier.journalBMC Microbiologyen
dc.description.collectioninformationThis item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.en
dc.eprint.versionFinal published versionen
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