Ancient eudicot hexaploidy meets ancestral eurosid gene order

Persistent Link:
http://hdl.handle.net/10150/610022
Title:
Ancient eudicot hexaploidy meets ancestral eurosid gene order
Author:
Zheng, Chunfang; Chen, Eric; Albert, Victor; Lyons, Eric; Sankoff, David
Affiliation:
Department of Mathematics and Statistics, University of Ottawa, 585 King Edward Avenue, Ottawa, Canada K1N 6N5; Department of Biology, University of Ottawa, 30 Marie-Curie, Ottawa, Canada K1N 6N5; Department of Biological Sciences, University at Buffalo, Buffalo, NY 14260, USA; School of Plant Sciences, University of Arizona, 1140 E. South Campus Drive, Tucson, AZ 85721, USA
Issue Date:
2013
Publisher:
BioMed Central
Citation:
Zheng et al. BMC Genomics 2013, 14(Suppl 7):S3 http://www.biomedcentral.com/1471-2164/14/S7/S3
Journal:
BMC Genomics
Rights:
© 2013 Zheng et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0)
Collection Information:
This item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.
Abstract:
BACKGROUND:A hexaploidization event over 125 Mya underlies the evolutionary lineage of the majority of flowering plants, including very many species of agricultural importance. Half of these belong to the rosid subgrouping, containing severals whose genome sequences have been published. Although most duplicate and triplicate genes have been lost in all descendants, clear traces of the original chromosome triples can be discerned, their internal contiguity highly conserved in some genomes and very fragmented in others. To understand the particular evolutionary patterns of plant genomes, there is a need to systematically survey the fate of the subgenomes of polyploids, including the retention of a small proportion of the duplicate and triplicate genes and the reconstruction of putative ancestral intermediates between the original hexaploid and modern species, in this case the ancestor of the eurosid clade.RESULTS:We quantitatively trace the fate of gene triples originating in the hexaploidy across seven core eudicot flowering plants, and fit this to a two-stage model, pre- and post-radiation. We also measure the simultaneous dynamics of duplicate orthologous gene loss in three rosids, as influenced by biological functional class. We propose a new protocol for reconstructing ancestral gene order using only gene adjacency data from pairwise genomic analyses, based on repeating MAXIMUM WEIGHT MATCHING at two levels of resolution, an approach designed to transcend limitations on reconstructed contig size, while still avoiding the ambiguities of a multiplicity of solutions. Applied to three high-quality rosid genomes without subsequent polyploidy events, our automated procedure reconstructs the ancestor of the eurosid clade.CONCLUSIONS:The gene loss analysis and the ancestor reconstruction present complementary assessments of post-hexaploidization evolution, the first at the level of individual gene families within and across sister genomes and the second at the chromosome level. Despite the loss of more than 95% of gene duplicates and triplicates, and despite major structural rearrangement, our reconstructed eurosid ancestor clearly identifies the three regions corresponding to each of the seven original chromosomes of the earlier pre-hexaploid ancestor. Functional analysis confirmed trends reported for more recent plant polyploidy events: genes involved with regulation and responses were retained in multiple copies, while genes involved with metabolic processes were lost.
EISSN:
1471-2164
DOI:
10.1186/1471-2164-14-S7-S3
Version:
Final published version
Additional Links:
http://www.biomedcentral.com/1471-2164/14/S7/S3

Full metadata record

DC FieldValue Language
dc.contributor.authorZheng, Chunfangen
dc.contributor.authorChen, Ericen
dc.contributor.authorAlbert, Victoren
dc.contributor.authorLyons, Ericen
dc.contributor.authorSankoff, Daviden
dc.date.accessioned2016-05-20T08:56:42Z-
dc.date.available2016-05-20T08:56:42Z-
dc.date.issued2013en
dc.identifier.citationZheng et al. BMC Genomics 2013, 14(Suppl 7):S3 http://www.biomedcentral.com/1471-2164/14/S7/S3en
dc.identifier.doi10.1186/1471-2164-14-S7-S3en
dc.identifier.urihttp://hdl.handle.net/10150/610022-
dc.description.abstractBACKGROUND:A hexaploidization event over 125 Mya underlies the evolutionary lineage of the majority of flowering plants, including very many species of agricultural importance. Half of these belong to the rosid subgrouping, containing severals whose genome sequences have been published. Although most duplicate and triplicate genes have been lost in all descendants, clear traces of the original chromosome triples can be discerned, their internal contiguity highly conserved in some genomes and very fragmented in others. To understand the particular evolutionary patterns of plant genomes, there is a need to systematically survey the fate of the subgenomes of polyploids, including the retention of a small proportion of the duplicate and triplicate genes and the reconstruction of putative ancestral intermediates between the original hexaploid and modern species, in this case the ancestor of the eurosid clade.RESULTS:We quantitatively trace the fate of gene triples originating in the hexaploidy across seven core eudicot flowering plants, and fit this to a two-stage model, pre- and post-radiation. We also measure the simultaneous dynamics of duplicate orthologous gene loss in three rosids, as influenced by biological functional class. We propose a new protocol for reconstructing ancestral gene order using only gene adjacency data from pairwise genomic analyses, based on repeating MAXIMUM WEIGHT MATCHING at two levels of resolution, an approach designed to transcend limitations on reconstructed contig size, while still avoiding the ambiguities of a multiplicity of solutions. Applied to three high-quality rosid genomes without subsequent polyploidy events, our automated procedure reconstructs the ancestor of the eurosid clade.CONCLUSIONS:The gene loss analysis and the ancestor reconstruction present complementary assessments of post-hexaploidization evolution, the first at the level of individual gene families within and across sister genomes and the second at the chromosome level. Despite the loss of more than 95% of gene duplicates and triplicates, and despite major structural rearrangement, our reconstructed eurosid ancestor clearly identifies the three regions corresponding to each of the seven original chromosomes of the earlier pre-hexaploid ancestor. Functional analysis confirmed trends reported for more recent plant polyploidy events: genes involved with regulation and responses were retained in multiple copies, while genes involved with metabolic processes were lost.en
dc.language.isoenen
dc.publisherBioMed Centralen
dc.relation.urlhttp://www.biomedcentral.com/1471-2164/14/S7/S3en
dc.rights© 2013 Zheng et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0)en
dc.titleAncient eudicot hexaploidy meets ancestral eurosid gene orderen
dc.typeArticleen
dc.identifier.eissn1471-2164en
dc.contributor.departmentDepartment of Mathematics and Statistics, University of Ottawa, 585 King Edward Avenue, Ottawa, Canada K1N 6N5en
dc.contributor.departmentDepartment of Biology, University of Ottawa, 30 Marie-Curie, Ottawa, Canada K1N 6N5en
dc.contributor.departmentDepartment of Biological Sciences, University at Buffalo, Buffalo, NY 14260, USAen
dc.contributor.departmentSchool of Plant Sciences, University of Arizona, 1140 E. South Campus Drive, Tucson, AZ 85721, USAen
dc.identifier.journalBMC Genomicsen
dc.description.collectioninformationThis item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.en
dc.eprint.versionFinal published versionen
All Items in UA Campus Repository are protected by copyright, with all rights reserved, unless otherwise indicated.