Bio-crude transcriptomics: Gene discovery and metabolic network reconstruction for the biosynthesis of the terpenome of the hydrocarbon oil-producing green alga, Botryococcus braunii race B (Showa)*

Persistent Link:
http://hdl.handle.net/10150/610020
Title:
Bio-crude transcriptomics: Gene discovery and metabolic network reconstruction for the biosynthesis of the terpenome of the hydrocarbon oil-producing green alga, Botryococcus braunii race B (Showa)*
Author:
Molnar, Istvan; Lopez, David; Wisecaver, Jennifer; Devarenne, Timothy; Weiss, Taylor; Pellegrini, Matteo; Hackett, Jeremiah
Affiliation:
Natural Products Center, School of Natural Resources and the Environment, The University of Arizona, 250 E. Valencia Rd, Tucson, AZ, 85739, USA; Bio5 Institute, The University of Arizona, 1657 E. Helen St, Tucson, AZ, 85721, USA; Department of Molecular, Cell and Developmental Biology, University of California Los Angeles, P. O. Box 951606, Los Angeles, CA, 90095, USA; Department of Ecology and Evolutionary Biology, The University of Arizona, 1041 E. Lowell St, Tucson, AZ, 85721, USA; Department of Biochemistry and Biophysics, Texas A&M University, 2128 TAMU, College Station, TX, 77843, USA
Issue Date:
2012
Publisher:
BioMed Central
Citation:
Molnár et al. BMC Genomics 2012, 13:576 http://www.biomedcentral.com/1471-2164/13/576
Journal:
BMC Genomics
Rights:
© 2012 Molnár et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0)
Collection Information:
This item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.
Abstract:
BACKGROUND:Microalgae hold promise for yielding a biofuel feedstock that is sustainable, carbon-neutral, distributed, and only minimally disruptive for the production of food and feed by traditional agriculture. Amongst oleaginous eukaryotic algae, the B race of Botryococcus braunii is unique in that it produces large amounts of liquid hydrocarbons of terpenoid origin. These are comparable to fossil crude oil, and are sequestered outside the cells in a communal extracellular polymeric matrix material. Biosynthetic engineering of terpenoid bio-crude production requires identification of genes and reconstruction of metabolic pathways responsible for production of both hydrocarbons and other metabolites of the alga that compete for photosynthetic carbon and energy.RESULTS:A de novo assembly of 1,334,609 next-generation pyrosequencing reads form the Showa strain of the B race of B. braunii yielded a transcriptomic database of 46,422 contigs with an average length of 756 bp. Contigs were annotated with pathway, ontology, and protein domain identifiers. Manual curation allowed the reconstruction of pathways that produce terpenoid liquid hydrocarbons from primary metabolites, and pathways that divert photosynthetic carbon into tetraterpenoid carotenoids, diterpenoids, and the prenyl chains of meroterpenoid quinones and chlorophyll. Inventories of machine-assembled contigs are also presented for reconstructed pathways for the biosynthesis of competing storage compounds including triacylglycerol and starch. Regeneration of S-adenosylmethionine, and the extracellular localization of the hydrocarbon oils by active transport and possibly autophagy are also investigated.CONCLUSIONS:The construction of an annotated transcriptomic database, publicly available in a web-based data depository and annotation tool, provides a foundation for metabolic pathway and network reconstruction, and facilitates further omics studies in the absence of a genome sequence for the Showa strain of B. braunii, race B. Further, the transcriptome database empowers future biosynthetic engineering approaches for strain improvement and the transfer of desirable traits to heterologous hosts.
EISSN:
1471-2164
DOI:
10.1186/1471-2164-13-576
Keywords:
Biofuel; Terpene biosynthesis; Fatty acid biosynthesis; Triacylglycerol biosynthesis; Starch biosynthesis; ABC transporter; Autophagy; Transcriptome; Botryococcus braunii; Botryococcene
Version:
Final published version
Additional Links:
http://www.biomedcentral.com/1471-2164/13/576

Full metadata record

DC FieldValue Language
dc.contributor.authorMolnar, Istvanen
dc.contributor.authorLopez, Daviden
dc.contributor.authorWisecaver, Jenniferen
dc.contributor.authorDevarenne, Timothyen
dc.contributor.authorWeiss, Tayloren
dc.contributor.authorPellegrini, Matteoen
dc.contributor.authorHackett, Jeremiahen
dc.date.accessioned2016-05-20T08:56:39Z-
dc.date.available2016-05-20T08:56:39Z-
dc.date.issued2012en
dc.identifier.citationMolnár et al. BMC Genomics 2012, 13:576 http://www.biomedcentral.com/1471-2164/13/576en
dc.identifier.doi10.1186/1471-2164-13-576en
dc.identifier.urihttp://hdl.handle.net/10150/610020-
dc.description.abstractBACKGROUND:Microalgae hold promise for yielding a biofuel feedstock that is sustainable, carbon-neutral, distributed, and only minimally disruptive for the production of food and feed by traditional agriculture. Amongst oleaginous eukaryotic algae, the B race of Botryococcus braunii is unique in that it produces large amounts of liquid hydrocarbons of terpenoid origin. These are comparable to fossil crude oil, and are sequestered outside the cells in a communal extracellular polymeric matrix material. Biosynthetic engineering of terpenoid bio-crude production requires identification of genes and reconstruction of metabolic pathways responsible for production of both hydrocarbons and other metabolites of the alga that compete for photosynthetic carbon and energy.RESULTS:A de novo assembly of 1,334,609 next-generation pyrosequencing reads form the Showa strain of the B race of B. braunii yielded a transcriptomic database of 46,422 contigs with an average length of 756 bp. Contigs were annotated with pathway, ontology, and protein domain identifiers. Manual curation allowed the reconstruction of pathways that produce terpenoid liquid hydrocarbons from primary metabolites, and pathways that divert photosynthetic carbon into tetraterpenoid carotenoids, diterpenoids, and the prenyl chains of meroterpenoid quinones and chlorophyll. Inventories of machine-assembled contigs are also presented for reconstructed pathways for the biosynthesis of competing storage compounds including triacylglycerol and starch. Regeneration of S-adenosylmethionine, and the extracellular localization of the hydrocarbon oils by active transport and possibly autophagy are also investigated.CONCLUSIONS:The construction of an annotated transcriptomic database, publicly available in a web-based data depository and annotation tool, provides a foundation for metabolic pathway and network reconstruction, and facilitates further omics studies in the absence of a genome sequence for the Showa strain of B. braunii, race B. Further, the transcriptome database empowers future biosynthetic engineering approaches for strain improvement and the transfer of desirable traits to heterologous hosts.en
dc.language.isoenen
dc.publisherBioMed Centralen
dc.relation.urlhttp://www.biomedcentral.com/1471-2164/13/576en
dc.rights© 2012 Molnár et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0)en
dc.subjectBiofuelen
dc.subjectTerpene biosynthesisen
dc.subjectFatty acid biosynthesisen
dc.subjectTriacylglycerol biosynthesisen
dc.subjectStarch biosynthesisen
dc.subjectABC transporteren
dc.subjectAutophagyen
dc.subjectTranscriptomeen
dc.subjectBotryococcus brauniien
dc.subjectBotryococceneen
dc.titleBio-crude transcriptomics: Gene discovery and metabolic network reconstruction for the biosynthesis of the terpenome of the hydrocarbon oil-producing green alga, Botryococcus braunii race B (Showa)*en
dc.typeArticleen
dc.identifier.eissn1471-2164en
dc.contributor.departmentNatural Products Center, School of Natural Resources and the Environment, The University of Arizona, 250 E. Valencia Rd, Tucson, AZ, 85739, USAen
dc.contributor.departmentBio5 Institute, The University of Arizona, 1657 E. Helen St, Tucson, AZ, 85721, USAen
dc.contributor.departmentDepartment of Molecular, Cell and Developmental Biology, University of California Los Angeles, P. O. Box 951606, Los Angeles, CA, 90095, USAen
dc.contributor.departmentDepartment of Ecology and Evolutionary Biology, The University of Arizona, 1041 E. Lowell St, Tucson, AZ, 85721, USAen
dc.contributor.departmentDepartment of Biochemistry and Biophysics, Texas A&M University, 2128 TAMU, College Station, TX, 77843, USAen
dc.identifier.journalBMC Genomicsen
dc.description.collectioninformationThis item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.en
dc.eprint.versionFinal published versionen
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