Identification of ejaculated proteins in the house mouse (Mus domesticus) via isotopic labeling

Persistent Link:
http://hdl.handle.net/10150/610018
Title:
Identification of ejaculated proteins in the house mouse (Mus domesticus) via isotopic labeling
Author:
Dean, Matthew; Findlay, Geoffrey; Hoopmann, Michael; Wu, Christine; MacCoss, Michael; Swanson, Willie; Nachman, Michael
Affiliation:
Molecular and Computational Biology, University of Southern California, 1050 Childs Way, Los Angeles, CA, USA; Ecology and Evolutionary Biology, University of Arizona, Tucson, AZ, USA; Department of Genome Sciences, University of Washington, Seattle, WA, USA; Department of Cell Biology, University of Pittsburgh, Pittsburgh, PA, USA
Issue Date:
2011
Publisher:
BioMed Central
Citation:
Dean et al. BMC Genomics 2011, 12:306 http://www.biomedcentral.com/1471-2164/12/306
Journal:
BMC Genomics
Rights:
© 2011 Dean et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0)
Collection Information:
This item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.
Abstract:
BACKGROUND:Seminal fluid plays an important role in successful fertilization, but knowledge of the full suite of proteins transferred from males to females during copulation is incomplete. The list of ejaculated proteins remains particularly scant in one of the best-studied mammalian systems, the house mouse (Mus domesticus), where artificial ejaculation techniques have proven inadequate. Here we investigate an alternative method for identifying ejaculated proteins, by isotopically labeling females with 15N and then mating them to unlabeled, vasectomized males. Proteins were then isolated from mated females and identified using mass spectrometry. In addition to gaining insights into possible functions and fates of ejaculated proteins, our study serves as proof of concept that isotopic labeling is a powerful means to study reproductive proteins.RESULTS:We identified 69 male-derived proteins from the female reproductive tract following copulation. More than a third of all spectra detected mapped to just seven genes known to be structurally important in the formation of the copulatory plug, a hard coagulum that forms shortly after mating. Seminal fluid is significantly enriched for proteins that function in protection from oxidative stress and endopeptidase inhibition. Females, on the other hand, produce endopeptidases in response to mating. The 69 ejaculated proteins evolve significantly more rapidly than other proteins that we previously identified directly from dissection of the male reproductive tract.CONCLUSION:Our study attempts to comprehensively identify the proteins transferred from males to females during mating, expanding the application of isotopic labeling to mammalian reproductive genomics. This technique opens the way to the targeted monitoring of the fate of ejaculated proteins as they incubate in the female reproductive tract.
EISSN:
1471-2164
DOI:
10.1186/1471-2164-12-306
Keywords:
seminal fluid; ejaculate; evolution
Version:
Final published version
Additional Links:
http://www.biomedcentral.com/1471-2164/12/306

Full metadata record

DC FieldValue Language
dc.contributor.authorDean, Matthewen
dc.contributor.authorFindlay, Geoffreyen
dc.contributor.authorHoopmann, Michaelen
dc.contributor.authorWu, Christineen
dc.contributor.authorMacCoss, Michaelen
dc.contributor.authorSwanson, Willieen
dc.contributor.authorNachman, Michaelen
dc.date.accessioned2016-05-20T08:56:35Z-
dc.date.available2016-05-20T08:56:35Z-
dc.date.issued2011en
dc.identifier.citationDean et al. BMC Genomics 2011, 12:306 http://www.biomedcentral.com/1471-2164/12/306en
dc.identifier.doi10.1186/1471-2164-12-306en
dc.identifier.urihttp://hdl.handle.net/10150/610018-
dc.description.abstractBACKGROUND:Seminal fluid plays an important role in successful fertilization, but knowledge of the full suite of proteins transferred from males to females during copulation is incomplete. The list of ejaculated proteins remains particularly scant in one of the best-studied mammalian systems, the house mouse (Mus domesticus), where artificial ejaculation techniques have proven inadequate. Here we investigate an alternative method for identifying ejaculated proteins, by isotopically labeling females with 15N and then mating them to unlabeled, vasectomized males. Proteins were then isolated from mated females and identified using mass spectrometry. In addition to gaining insights into possible functions and fates of ejaculated proteins, our study serves as proof of concept that isotopic labeling is a powerful means to study reproductive proteins.RESULTS:We identified 69 male-derived proteins from the female reproductive tract following copulation. More than a third of all spectra detected mapped to just seven genes known to be structurally important in the formation of the copulatory plug, a hard coagulum that forms shortly after mating. Seminal fluid is significantly enriched for proteins that function in protection from oxidative stress and endopeptidase inhibition. Females, on the other hand, produce endopeptidases in response to mating. The 69 ejaculated proteins evolve significantly more rapidly than other proteins that we previously identified directly from dissection of the male reproductive tract.CONCLUSION:Our study attempts to comprehensively identify the proteins transferred from males to females during mating, expanding the application of isotopic labeling to mammalian reproductive genomics. This technique opens the way to the targeted monitoring of the fate of ejaculated proteins as they incubate in the female reproductive tract.en
dc.language.isoenen
dc.publisherBioMed Centralen
dc.relation.urlhttp://www.biomedcentral.com/1471-2164/12/306en
dc.rights© 2011 Dean et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0)en
dc.subjectseminal fluiden
dc.subjectejaculateen
dc.subjectevolutionen
dc.titleIdentification of ejaculated proteins in the house mouse (Mus domesticus) via isotopic labelingen
dc.typeArticleen
dc.identifier.eissn1471-2164en
dc.contributor.departmentMolecular and Computational Biology, University of Southern California, 1050 Childs Way, Los Angeles, CA, USAen
dc.contributor.departmentEcology and Evolutionary Biology, University of Arizona, Tucson, AZ, USAen
dc.contributor.departmentDepartment of Genome Sciences, University of Washington, Seattle, WA, USAen
dc.contributor.departmentDepartment of Cell Biology, University of Pittsburgh, Pittsburgh, PA, USAen
dc.identifier.journalBMC Genomicsen
dc.description.collectioninformationThis item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.en
dc.eprint.versionFinal published versionen
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