The Alveolate Perkinsus marinus: Biological Insights from EST Gene Discovery

Persistent Link:
http://hdl.handle.net/10150/610012
Title:
The Alveolate Perkinsus marinus: Biological Insights from EST Gene Discovery
Author:
Joseph, Sandeep; Fernandez-Robledo, Jose; Gardner, Malcolm; El-Sayed, Najib; Kuo, Chih-Horng; Schott, Eric; Wang, Haiming; Kissinger, Jessica; Vasta, Gerardo
Affiliation:
Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA; Department of Genetics, University of Georgia, Athens, GA 30602, USA; Department of Microbiology and Immunology, University of Maryland School of Medicine, IMET, Baltimore, MD 21202, USA; J. Craig Venter Institute, formerly Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA; Current address: Department of Medicine, Division of Infectious Diseases, Emory University School of Medicine, Atlanta, GA 30322, USA; Current address: Seattle Biomedical Research Institute, Seattle, WA 98109, USA; Current address: Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA; Current address: Department of Chemistry and Biochemistry, University of Arizona, Tucson, AZ 85721, USA; Current address: University of Maryland Center for Environmental Science, IMET, Baltimore, MD 21202, USA
Issue Date:
2010
Publisher:
BioMed Central
Citation:
Joseph et al. BMC Genomics 2010, 11:228 http://www.biomedcentral.com/1471-2164/11/228
Journal:
BMC Genomics
Rights:
© 2010 Joseph et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0)
Collection Information:
This item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.
Abstract:
BACKGROUND:Perkinsus marinus, a protozoan parasite of the eastern oyster Crassostrea virginica, has devastated natural and farmed oyster populations along the Atlantic and Gulf coasts of the United States. It is classified as a member of the Perkinsozoa, a recently established phylum considered close to the ancestor of ciliates, dinoflagellates, and apicomplexans, and a key taxon for understanding unique adaptations (e.g. parasitism) within the Alveolata. Despite intense parasite pressure, no disease-resistant oysters have been identified and no effective therapies have been developed to date.RESULTS:To gain insight into the biological basis of the parasite's virulence and pathogenesis mechanisms, and to identify genes encoding potential targets for intervention, we generated >31,000 5' expressed sequence tags (ESTs) derived from four trophozoite libraries generated from two P. marinus strains. Trimming and clustering of the sequence tags yielded 7,863 unique sequences, some of which carry a spliced leader. Similarity searches revealed that 55% of these had hits in protein sequence databases, of which 1,729 had their best hit with proteins from the chromalveolates (E-value less than or equal to] 1e-5). Some sequences are similar to those proven to be targets for effective intervention in other protozoan parasites, and include not only proteases, antioxidant enzymes, and heat shock proteins, but also those associated with relict plastids, such as acetyl-CoA carboxylase and methyl erythrithol phosphate pathway components, and those involved in glycan assembly, protein folding/secretion, and parasite-host interactions.CONCLUSIONS:Our transcriptome analysis of P. marinus, the first for any member of the Perkinsozoa, contributes new insight into its biology and taxonomic position. It provides a very informative, albeit preliminary, glimpse into the expression of genes encoding functionally relevant proteins as potential targets for chemotherapy, and evidence for the presence of a relict plastid. Further, although P. marinus sequences display significant similarity to those from both apicomplexans and dinoflagellates, the presence of trans-spliced transcripts confirms the previously established affinities with the latter. The EST analysis reported herein, together with the recently completed sequence of the P. marinus genome and the development of transfection methodology, should result in improved intervention strategies against dermo disease.
EISSN:
1471-2164
DOI:
10.1186/1471-2164-11-228
Version:
Final published version
Additional Links:
http://www.biomedcentral.com/1471-2164/11/228

Full metadata record

DC FieldValue Language
dc.contributor.authorJoseph, Sandeepen
dc.contributor.authorFernandez-Robledo, Joseen
dc.contributor.authorGardner, Malcolmen
dc.contributor.authorEl-Sayed, Najiben
dc.contributor.authorKuo, Chih-Horngen
dc.contributor.authorSchott, Ericen
dc.contributor.authorWang, Haimingen
dc.contributor.authorKissinger, Jessicaen
dc.contributor.authorVasta, Gerardoen
dc.date.accessioned2016-05-20T08:56:27Z-
dc.date.available2016-05-20T08:56:27Z-
dc.date.issued2010en
dc.identifier.citationJoseph et al. BMC Genomics 2010, 11:228 http://www.biomedcentral.com/1471-2164/11/228en
dc.identifier.doi10.1186/1471-2164-11-228en
dc.identifier.urihttp://hdl.handle.net/10150/610012-
dc.description.abstractBACKGROUND:Perkinsus marinus, a protozoan parasite of the eastern oyster Crassostrea virginica, has devastated natural and farmed oyster populations along the Atlantic and Gulf coasts of the United States. It is classified as a member of the Perkinsozoa, a recently established phylum considered close to the ancestor of ciliates, dinoflagellates, and apicomplexans, and a key taxon for understanding unique adaptations (e.g. parasitism) within the Alveolata. Despite intense parasite pressure, no disease-resistant oysters have been identified and no effective therapies have been developed to date.RESULTS:To gain insight into the biological basis of the parasite's virulence and pathogenesis mechanisms, and to identify genes encoding potential targets for intervention, we generated >31,000 5' expressed sequence tags (ESTs) derived from four trophozoite libraries generated from two P. marinus strains. Trimming and clustering of the sequence tags yielded 7,863 unique sequences, some of which carry a spliced leader. Similarity searches revealed that 55% of these had hits in protein sequence databases, of which 1,729 had their best hit with proteins from the chromalveolates (E-value less than or equal to] 1e-5). Some sequences are similar to those proven to be targets for effective intervention in other protozoan parasites, and include not only proteases, antioxidant enzymes, and heat shock proteins, but also those associated with relict plastids, such as acetyl-CoA carboxylase and methyl erythrithol phosphate pathway components, and those involved in glycan assembly, protein folding/secretion, and parasite-host interactions.CONCLUSIONS:Our transcriptome analysis of P. marinus, the first for any member of the Perkinsozoa, contributes new insight into its biology and taxonomic position. It provides a very informative, albeit preliminary, glimpse into the expression of genes encoding functionally relevant proteins as potential targets for chemotherapy, and evidence for the presence of a relict plastid. Further, although P. marinus sequences display significant similarity to those from both apicomplexans and dinoflagellates, the presence of trans-spliced transcripts confirms the previously established affinities with the latter. The EST analysis reported herein, together with the recently completed sequence of the P. marinus genome and the development of transfection methodology, should result in improved intervention strategies against dermo disease.en
dc.language.isoenen
dc.publisherBioMed Centralen
dc.relation.urlhttp://www.biomedcentral.com/1471-2164/11/228en
dc.rights© 2010 Joseph et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0)en
dc.titleThe Alveolate Perkinsus marinus: Biological Insights from EST Gene Discoveryen
dc.typeArticleen
dc.identifier.eissn1471-2164en
dc.contributor.departmentCenter for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA 30602, USAen
dc.contributor.departmentDepartment of Genetics, University of Georgia, Athens, GA 30602, USAen
dc.contributor.departmentDepartment of Microbiology and Immunology, University of Maryland School of Medicine, IMET, Baltimore, MD 21202, USAen
dc.contributor.departmentJ. Craig Venter Institute, formerly Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USAen
dc.contributor.departmentCurrent address: Department of Medicine, Division of Infectious Diseases, Emory University School of Medicine, Atlanta, GA 30322, USAen
dc.contributor.departmentCurrent address: Seattle Biomedical Research Institute, Seattle, WA 98109, USAen
dc.contributor.departmentCurrent address: Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USAen
dc.contributor.departmentCurrent address: Department of Chemistry and Biochemistry, University of Arizona, Tucson, AZ 85721, USAen
dc.contributor.departmentCurrent address: University of Maryland Center for Environmental Science, IMET, Baltimore, MD 21202, USAen
dc.identifier.journalBMC Genomicsen
dc.description.collectioninformationThis item is part of the UA Faculty Publications collection. For more information this item or other items in the UA Campus Repository, contact the University of Arizona Libraries at repository@u.library.arizona.edu.en
dc.eprint.versionFinal published versionen
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