Persistent Link:
http://hdl.handle.net/10150/594936
Title:
The Genetic Basis for Biofilm Formation of Pseudomonas Stutzeri
Author:
Ainscow, Clarice Anna
Issue Date:
2015
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Pseudomonas stutzeri is evaluated in this thesis for its ability to form a coral, corrugated biofilm. It begins with an overview of biofilm formation in other species, then offers a more concentrated view on the pseudomonad group, to give context for this topic. The three experiments performed were a nitrate experiment to test if a lack of access to nitrate influenced the coral biofilm formation, a passage experiment to show how colony morphology changed in pellicle formation in liquid media over time, and a transposon mutagenesis experiment to insert a transposon into the gene(s) involved in coral biofilm formation to knockout the ability. The nitrate experiment showed no difference between colony morphology on plates with and without nitrate added. The passage experiment showed coral biofilm formation ability being lost in some colonies as early as one week after inoculation and a great diversity of morphologic characteristics appeared over time. Pellicle formation also lost robustness over time with some colonies becoming more motile and other hyper-biofilm forming. The transposon mutagenesis experiment did not come to completion due to colonies losing coral biofilm formation ability on their own without necessarily being caused by the transposon. Sequence data is still currently being processed.
Type:
text; Electronic Thesis
Degree Name:
B.S.
Degree Level:
bachelors
Degree Program:
Honors College; Biology: Biomedical Sciences Emphasis
Degree Grantor:
University of Arizona
Advisor:
Baltrus, David

Full metadata record

DC FieldValue Language
dc.language.isoen_USen
dc.titleThe Genetic Basis for Biofilm Formation of Pseudomonas Stutzerien_US
dc.creatorAinscow, Clarice Annaen
dc.contributor.authorAinscow, Clarice Annaen
dc.date.issued2015en
dc.publisherThe University of Arizona.en
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en
dc.description.abstractPseudomonas stutzeri is evaluated in this thesis for its ability to form a coral, corrugated biofilm. It begins with an overview of biofilm formation in other species, then offers a more concentrated view on the pseudomonad group, to give context for this topic. The three experiments performed were a nitrate experiment to test if a lack of access to nitrate influenced the coral biofilm formation, a passage experiment to show how colony morphology changed in pellicle formation in liquid media over time, and a transposon mutagenesis experiment to insert a transposon into the gene(s) involved in coral biofilm formation to knockout the ability. The nitrate experiment showed no difference between colony morphology on plates with and without nitrate added. The passage experiment showed coral biofilm formation ability being lost in some colonies as early as one week after inoculation and a great diversity of morphologic characteristics appeared over time. Pellicle formation also lost robustness over time with some colonies becoming more motile and other hyper-biofilm forming. The transposon mutagenesis experiment did not come to completion due to colonies losing coral biofilm formation ability on their own without necessarily being caused by the transposon. Sequence data is still currently being processed.en
dc.typetexten
dc.typeElectronic Thesisen
thesis.degree.nameB.S.en
thesis.degree.levelbachelorsen
thesis.degree.disciplineHonors Collegeen
thesis.degree.disciplineBiology: Biomedical Sciences Emphasisen
thesis.degree.grantorUniversity of Arizonaen
dc.contributor.advisorBaltrus, Daviden
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