ncRNA Targeting CSF-1 and CSF1R Expression in Breast and Ovarian Cancer Cells

Persistent Link:
http://hdl.handle.net/10150/579336
Title:
ncRNA Targeting CSF-1 and CSF1R Expression in Breast and Ovarian Cancer Cells
Author:
Patel, Arpan Ajit
Issue Date:
2015
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Various sense ncRNA transcripts targeting nucleolin and vigilin, and shRNA transcripts targeting CSF-1 and c-fms mRNAs were introduced into Hey ovarian cancer and BT20 breast cancer cell lines to measure their influence on CSF1R and CSF1 protein expression. In BT20 cells transcripts c-fms-C-rich 104-S, c-fms-(CS-rCS), and c-fms-Py-S were found to downregulate CSF1R expression. In BT20 cells transcripts CSF-1-Exon-10-356-S and CSF-1 (CS-rCS) were found to have a downregulatory effect on CSF1 expression while transcript CSF-1-5'UTR-420-S was found to upregulate CSF1 expression. In Hey cells transcript CSF-1-3'UTR-AS-Pyrimidine was found to downregulate CSF1 expression while transcript CSF-1mRNA-Variant-4-5'UTR was found to have upregulate CSF1 expression. Also in Hey cells, both transcripts c-fms C-rich-104-S and c-fms-Py-S were found to upregulate CSF1R expression. These results indicate that ncRNAs are able to have a regulatory effect on CSF1 and CSF1R expression but also suggest that the mechanism of regulation is complex. Furthermore, the complex mechanism makes it difficult to predict if the transcript will have its intended effect. We concluded that differences in baseline RNA binding protein concentrations and other regulatory mechanisms, such as microRNAs, account for the conflicting results between the BT20 and Hey cell lines when comparing the same inserted transcript.
Type:
text; Electronic Thesis
Degree Name:
B.S.
Degree Level:
bachelors
Degree Program:
Honors College; Molecular and Cellular Biology
Degree Grantor:
University of Arizona
Advisor:
Chambers, Setsuko; Woo, Ho-Hyung

Full metadata record

DC FieldValue Language
dc.language.isoen_USen
dc.titlencRNA Targeting CSF-1 and CSF1R Expression in Breast and Ovarian Cancer Cellsen_US
dc.creatorPatel, Arpan Ajiten
dc.contributor.authorPatel, Arpan Ajiten
dc.date.issued2015en
dc.publisherThe University of Arizona.en
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en
dc.description.abstractVarious sense ncRNA transcripts targeting nucleolin and vigilin, and shRNA transcripts targeting CSF-1 and c-fms mRNAs were introduced into Hey ovarian cancer and BT20 breast cancer cell lines to measure their influence on CSF1R and CSF1 protein expression. In BT20 cells transcripts c-fms-C-rich 104-S, c-fms-(CS-rCS), and c-fms-Py-S were found to downregulate CSF1R expression. In BT20 cells transcripts CSF-1-Exon-10-356-S and CSF-1 (CS-rCS) were found to have a downregulatory effect on CSF1 expression while transcript CSF-1-5'UTR-420-S was found to upregulate CSF1 expression. In Hey cells transcript CSF-1-3'UTR-AS-Pyrimidine was found to downregulate CSF1 expression while transcript CSF-1mRNA-Variant-4-5'UTR was found to have upregulate CSF1 expression. Also in Hey cells, both transcripts c-fms C-rich-104-S and c-fms-Py-S were found to upregulate CSF1R expression. These results indicate that ncRNAs are able to have a regulatory effect on CSF1 and CSF1R expression but also suggest that the mechanism of regulation is complex. Furthermore, the complex mechanism makes it difficult to predict if the transcript will have its intended effect. We concluded that differences in baseline RNA binding protein concentrations and other regulatory mechanisms, such as microRNAs, account for the conflicting results between the BT20 and Hey cell lines when comparing the same inserted transcript.en
dc.typetexten
dc.typeElectronic Thesisen
thesis.degree.nameB.S.en
thesis.degree.levelbachelorsen
thesis.degree.disciplineHonors Collegeen
thesis.degree.disciplineMolecular and Cellular Biologyen
thesis.degree.grantorUniversity of Arizonaen
dc.contributor.advisorChambers, Setsukoen
dc.contributor.advisorWoo, Ho-Hyungen
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