Persistent Link:
http://hdl.handle.net/10150/579290
Title:
Study of the Structure of Rhodopsin during Photoactivation
Author:
Lovely, Sabrina Segui
Issue Date:
2015
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
G-protein coupled receptors are a superfamily of transmembrane proteins that are heavily involved in signal transduction within cells. They are extremely important to study because they are involved in many physiological processes such as sight, smell, taste, etc. GPCRs are also heavily targeted by drugs and therefore understanding the way they function can help improvements with the development of drugs. This study uses Bovine Rhodopsin as a prototypical model GPCR, as it is very commonly studied. Rhodopsin has an inactive Metarhodopsin I and an active Metarhodopsin II state that allow for the functionality of the protein. The equilibrium that exists between the two states are studied in order to observe the structural features of the activation process. Changing the metal cofactors in solution, and varying the osmotic pressure, can cause a shift in the equilibrium. The effect these factors have on the shift are studied in these experiments. Analyzing the different Meta I and Meta II states can be done by using UV-visible spectroscopy. The different environments created by the factors listed above will be studied and can be applied to other GPCR proteins as well.
Type:
text; Electronic Thesis
Degree Name:
B.S.
Degree Level:
bachelors
Degree Program:
Honors College; Biochemistry
Degree Grantor:
University of Arizona
Advisor:
Brown, Michael

Full metadata record

DC FieldValue Language
dc.language.isoen_USen
dc.titleStudy of the Structure of Rhodopsin during Photoactivationen_US
dc.creatorLovely, Sabrina Seguien
dc.contributor.authorLovely, Sabrina Seguien
dc.date.issued2015en
dc.publisherThe University of Arizona.en
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en
dc.description.abstractG-protein coupled receptors are a superfamily of transmembrane proteins that are heavily involved in signal transduction within cells. They are extremely important to study because they are involved in many physiological processes such as sight, smell, taste, etc. GPCRs are also heavily targeted by drugs and therefore understanding the way they function can help improvements with the development of drugs. This study uses Bovine Rhodopsin as a prototypical model GPCR, as it is very commonly studied. Rhodopsin has an inactive Metarhodopsin I and an active Metarhodopsin II state that allow for the functionality of the protein. The equilibrium that exists between the two states are studied in order to observe the structural features of the activation process. Changing the metal cofactors in solution, and varying the osmotic pressure, can cause a shift in the equilibrium. The effect these factors have on the shift are studied in these experiments. Analyzing the different Meta I and Meta II states can be done by using UV-visible spectroscopy. The different environments created by the factors listed above will be studied and can be applied to other GPCR proteins as well.en
dc.typetexten
dc.typeElectronic Thesisen
thesis.degree.nameB.S.en
thesis.degree.levelbachelorsen
thesis.degree.disciplineHonors Collegeen
thesis.degree.disciplineBiochemistryen
thesis.degree.grantorUniversity of Arizonaen
dc.contributor.advisorBrown, Michaelen
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