A Hybrid Electrokinetic Bioprocessor For Single-Cell Antimicrobial Susceptibility Testing

Persistent Link:
http://hdl.handle.net/10150/579108
Title:
A Hybrid Electrokinetic Bioprocessor For Single-Cell Antimicrobial Susceptibility Testing
Author:
Lu, Yi
Issue Date:
2015
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Embargo:
Release 21-Jan-2016
Abstract:
Infectious diseases resulting from bacterial pathogens are the most common causes of patient morbidity and mortality worldwide. The rapid identification of the pathogens and their antibiotic resistances is crucial for proper clinical management. However, the standard culture-based diagnostic approach requires a minimum of two days from the initial specimen collection to result reporting. As a consequence, broad-spectrum antibiotics are often prescribed under the worst-case assumption without knowledge of the pathogens or their resistances. The current clinical practice results in improper treatment of the patient and causes the rapid emergence of multi-drug resistant pathogens. A rapid diagnostics system has therefore been developed which performs hybrid electrokinetic sample preparation and volume reduction, for single-cell antimicrobial susceptibility testing (AST). The system combines multiple electrokinetic forces for sample preparation, which reduces the sample volume for over 3 orders of magnitude and minimizes the matrix effects of physiological samples for enhanced sensitivity. The device is integrated with a single-cell AST system with microfluidic confinement and electrokinetic loading to phenotypically determine the bacterial antibiotic resistance at the single-cell level. The applicability of the system has been demonstrated for performing direct AST with urine and blood samples within one hour, enabling rapid infectious disease diagnostics in non-traditional healthcare settings.
Type:
text; Electronic Dissertation
Keywords:
antibiotic resistance; antimicrobial susceptibility testing; Electrokinetics; microchannel; single cell; Mechanical Engineering; AC electrothermal flow
Degree Name:
Ph.D.
Degree Level:
doctoral
Degree Program:
Graduate College; Mechanical Engineering
Degree Grantor:
University of Arizona
Advisor:
Wong, Pak Kin

Full metadata record

DC FieldValue Language
dc.language.isoen_USen
dc.titleA Hybrid Electrokinetic Bioprocessor For Single-Cell Antimicrobial Susceptibility Testingen_US
dc.creatorLu, Yien
dc.contributor.authorLu, Yien
dc.date.issued2015en
dc.publisherThe University of Arizona.en
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en
dc.description.releaseRelease 21-Jan-2016en
dc.description.abstractInfectious diseases resulting from bacterial pathogens are the most common causes of patient morbidity and mortality worldwide. The rapid identification of the pathogens and their antibiotic resistances is crucial for proper clinical management. However, the standard culture-based diagnostic approach requires a minimum of two days from the initial specimen collection to result reporting. As a consequence, broad-spectrum antibiotics are often prescribed under the worst-case assumption without knowledge of the pathogens or their resistances. The current clinical practice results in improper treatment of the patient and causes the rapid emergence of multi-drug resistant pathogens. A rapid diagnostics system has therefore been developed which performs hybrid electrokinetic sample preparation and volume reduction, for single-cell antimicrobial susceptibility testing (AST). The system combines multiple electrokinetic forces for sample preparation, which reduces the sample volume for over 3 orders of magnitude and minimizes the matrix effects of physiological samples for enhanced sensitivity. The device is integrated with a single-cell AST system with microfluidic confinement and electrokinetic loading to phenotypically determine the bacterial antibiotic resistance at the single-cell level. The applicability of the system has been demonstrated for performing direct AST with urine and blood samples within one hour, enabling rapid infectious disease diagnostics in non-traditional healthcare settings.en
dc.typetexten
dc.typeElectronic Dissertationen
dc.subjectantibiotic resistanceen
dc.subjectantimicrobial susceptibility testingen
dc.subjectElectrokineticsen
dc.subjectmicrochannelen
dc.subjectsingle cellen
dc.subjectMechanical Engineeringen
dc.subjectAC electrothermal flowen
thesis.degree.namePh.D.en
thesis.degree.leveldoctoralen
thesis.degree.disciplineGraduate Collegeen
thesis.degree.disciplineMechanical Engineeringen
thesis.degree.grantorUniversity of Arizonaen
dc.contributor.advisorWong, Pak Kinen
dc.contributor.committeememberWong, Pak Kinen
dc.contributor.committeememberChan, Cho Liken
dc.contributor.committeememberZohar, Yitshaken
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