Effects of Variant Levels of Hypoxic Stress on Human Pancreatic Islets

Persistent Link:
http://hdl.handle.net/10150/579013
Title:
Effects of Variant Levels of Hypoxic Stress on Human Pancreatic Islets
Author:
Assimacopoulos, Aikaterina Lynn
Issue Date:
2015
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
The objective was to measure the effects of varying time points of pellet-induced hypoxic¹ exposure on human pancreatic islets through viability and function as compared to normoxic conditions. Hypoxic conditions were induced by storing the islets in pelletized conditions by which the islets lacked proper access to both oxygen and media. The islets were cultured for varying time points and then allowed to recover. The effects were measured upon immediate normalization of conditions as well as 24 or 48 hours later. When the islets were subjected for 12 hours, they showed an inability to recover through lack of both viability and function. The islets exposed to hypoxic conditions for only 4 hours were unable to recover any function, though no significant detriment to their viability was detectable. This would suggest that even as little as four hours of hypoxic exposure causes a naturally irreversible molecular change that damages cell function, even if no detectable lack of viability is present.
Type:
text; Electronic Thesis
Degree Name:
B.S.
Degree Level:
bachelors
Degree Program:
Honors College; Molecular and Cellular Biology
Degree Grantor:
University of Arizona
Advisor:
Papas, Klearchos K.

Full metadata record

DC FieldValue Language
dc.language.isoen_USen
dc.titleEffects of Variant Levels of Hypoxic Stress on Human Pancreatic Isletsen_US
dc.creatorAssimacopoulos, Aikaterina Lynnen
dc.contributor.authorAssimacopoulos, Aikaterina Lynnen
dc.date.issued2015en
dc.publisherThe University of Arizona.en
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en
dc.description.abstractThe objective was to measure the effects of varying time points of pellet-induced hypoxic¹ exposure on human pancreatic islets through viability and function as compared to normoxic conditions. Hypoxic conditions were induced by storing the islets in pelletized conditions by which the islets lacked proper access to both oxygen and media. The islets were cultured for varying time points and then allowed to recover. The effects were measured upon immediate normalization of conditions as well as 24 or 48 hours later. When the islets were subjected for 12 hours, they showed an inability to recover through lack of both viability and function. The islets exposed to hypoxic conditions for only 4 hours were unable to recover any function, though no significant detriment to their viability was detectable. This would suggest that even as little as four hours of hypoxic exposure causes a naturally irreversible molecular change that damages cell function, even if no detectable lack of viability is present.en
dc.typetexten
dc.typeElectronic Thesisen
thesis.degree.nameB.S.en
thesis.degree.levelbachelorsen
thesis.degree.disciplineHonors Collegeen
thesis.degree.disciplineMolecular and Cellular Biologyen
thesis.degree.grantorUniversity of Arizonaen
dc.contributor.advisorPapas, Klearchos K.en
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