The Implications of Knocking Out Two Major Salivary Androgen-Binding Protein Genes for Mouse Viability, Fecundity and the Expression of Other ABP Genes

Persistent Link:
http://hdl.handle.net/10150/578984
Title:
The Implications of Knocking Out Two Major Salivary Androgen-Binding Protein Genes for Mouse Viability, Fecundity and the Expression of Other ABP Genes
Author:
Chung, Amanda Grace
Issue Date:
2015
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
The Androgen-binding protein (Abp) genes in the house mouse genome have undergone extensive duplications that produced 64 Abp paralogs. Distinct sets of Abp genes are expressed in lacrimal and salivary glands. This study examines the changes in a mouse line lacking two major salivary Abp genes, Abpa27 and Abpbg27 (a27/bg27 knockout line). We observed no marked changes in lifespan or fecundity between the three genotypes. We paired traditional PCR with quantitative PCR to measure changes in Abp paralog expression in both submandibular and lacrimal glands. We observed a decrease in expression of a27 and bg27 transcripts in the salivary glands of +/- mice and a complete absence of a27 and bg27 in the salivary glands of -/- mice. We did not detect any changes in expression of any other Abp paralogs in salivary glands. On the other hand, we saw variance in Abp expression between the three genotypes in the lacrimal glands including an increase in bg10 and bg20 expression in female -/- mice, and a3 in male -/- mice. This study will be the first of many documenting the changes that occur in a knockout mouse line.
Type:
text; Electronic Thesis
Degree Name:
B.S.
Degree Level:
bachelors
Degree Program:
Honors College; Physiology
Degree Grantor:
University of Arizona
Advisor:
Laukaitis, Christina

Full metadata record

DC FieldValue Language
dc.language.isoen_USen
dc.titleThe Implications of Knocking Out Two Major Salivary Androgen-Binding Protein Genes for Mouse Viability, Fecundity and the Expression of Other ABP Genesen_US
dc.creatorChung, Amanda Graceen
dc.contributor.authorChung, Amanda Graceen
dc.date.issued2015en
dc.publisherThe University of Arizona.en
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en
dc.description.abstractThe Androgen-binding protein (Abp) genes in the house mouse genome have undergone extensive duplications that produced 64 Abp paralogs. Distinct sets of Abp genes are expressed in lacrimal and salivary glands. This study examines the changes in a mouse line lacking two major salivary Abp genes, Abpa27 and Abpbg27 (a27/bg27 knockout line). We observed no marked changes in lifespan or fecundity between the three genotypes. We paired traditional PCR with quantitative PCR to measure changes in Abp paralog expression in both submandibular and lacrimal glands. We observed a decrease in expression of a27 and bg27 transcripts in the salivary glands of +/- mice and a complete absence of a27 and bg27 in the salivary glands of -/- mice. We did not detect any changes in expression of any other Abp paralogs in salivary glands. On the other hand, we saw variance in Abp expression between the three genotypes in the lacrimal glands including an increase in bg10 and bg20 expression in female -/- mice, and a3 in male -/- mice. This study will be the first of many documenting the changes that occur in a knockout mouse line.en
dc.typetexten
dc.typeElectronic Thesisen
thesis.degree.nameB.S.en
thesis.degree.levelbachelorsen
thesis.degree.disciplineHonors Collegeen
thesis.degree.disciplinePhysiologyen
thesis.degree.grantorUniversity of Arizonaen
dc.contributor.advisorLaukaitis, Christinaen
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