Characterization and Applications for A Polymerized DiaCEST Contrast Agent

Persistent Link:
http://hdl.handle.net/10150/577329
Title:
Characterization and Applications for A Polymerized DiaCEST Contrast Agent
Author:
Bontrager, Jordan G.
Issue Date:
2015
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
MRI can benefit from an increase in the sensitivity of contrast agents. The CEST MRI technique in particular suffers from very poor sensitivity when using diamagnetic contrast agents. Polymerized CEST MRI contrast agents could increase the sensitivity per macromolecule over monomer contrast agents. The increase in sensitivity is related to the increase in number of contrast agents per polymer. A contrast agent with increased sensitivity can be used to image on the molecular level in vivo, where the concentration of targets is very low. A polymerized diaCEST contrast agent was synthesized by coupling a salicylic acid analogue to a poly (acrylic acid) backbone. The CEST effect of the coupled analogue was compared to its uncoupled form for different concentrations and pH values. A RL-QUEST method was used to calculate the exchange rate of the analogue for different pH values before and after coupling. The polymerized diaCEST agent was attempted to be loaded into DOPC and bis-SorbPC liposomes, and was also attempted to be targeted to folate receptors in a KB cell culture. These studies establish the foundation for translation of polymerized diaCEST contrast agents to additional in vitro and in vivo investigations.
Type:
text; Electronic Thesis
Keywords:
diaCEST; polymer; QUEST; salicylic acid; sensitivity; Biomedical Engineering; contrast agent
Degree Name:
M.S.
Degree Level:
masters
Degree Program:
Graduate College; Biomedical Engineering
Degree Grantor:
University of Arizona
Advisor:
Pagel, Mark D.

Full metadata record

DC FieldValue Language
dc.language.isoen_USen
dc.titleCharacterization and Applications for A Polymerized DiaCEST Contrast Agenten_US
dc.creatorBontrager, Jordan G.en
dc.contributor.authorBontrager, Jordan G.en
dc.date.issued2015en
dc.publisherThe University of Arizona.en
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en
dc.description.abstractMRI can benefit from an increase in the sensitivity of contrast agents. The CEST MRI technique in particular suffers from very poor sensitivity when using diamagnetic contrast agents. Polymerized CEST MRI contrast agents could increase the sensitivity per macromolecule over monomer contrast agents. The increase in sensitivity is related to the increase in number of contrast agents per polymer. A contrast agent with increased sensitivity can be used to image on the molecular level in vivo, where the concentration of targets is very low. A polymerized diaCEST contrast agent was synthesized by coupling a salicylic acid analogue to a poly (acrylic acid) backbone. The CEST effect of the coupled analogue was compared to its uncoupled form for different concentrations and pH values. A RL-QUEST method was used to calculate the exchange rate of the analogue for different pH values before and after coupling. The polymerized diaCEST agent was attempted to be loaded into DOPC and bis-SorbPC liposomes, and was also attempted to be targeted to folate receptors in a KB cell culture. These studies establish the foundation for translation of polymerized diaCEST contrast agents to additional in vitro and in vivo investigations.en
dc.typetexten
dc.typeElectronic Thesisen
dc.subjectdiaCESTen
dc.subjectpolymeren
dc.subjectQUESTen
dc.subjectsalicylic aciden
dc.subjectsensitivityen
dc.subjectBiomedical Engineeringen
dc.subjectcontrast agenten
thesis.degree.nameM.S.en
thesis.degree.levelmastersen
thesis.degree.disciplineGraduate Collegeen
thesis.degree.disciplineBiomedical Engineeringen
thesis.degree.grantorUniversity of Arizonaen
dc.contributor.advisorPagel, Mark D.en
dc.contributor.committeememberPagel, Mark D.en
dc.contributor.committeememberAspinwall, Craigen
dc.contributor.committeememberCardenas-Rodriguez, Julioen
dc.contributor.committeememberKuo, Phillipen
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