Bioinformatic Analysis of Angiotensin II Receptor Type 2 Expression and Its Potential Role in Neuropathic Pain

Persistent Link:
http://hdl.handle.net/10150/560826
Title:
Bioinformatic Analysis of Angiotensin II Receptor Type 2 Expression and Its Potential Role in Neuropathic Pain
Author:
Shy, Adia
Issue Date:
2015
Publisher:
The University of Arizona.
Rights:
Copyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.
Abstract:
Neuropathic pain is a tremendous medical problem that afflicts millions. It is also distinct from other pain conditions. It persists in the absence of non-noxious stimuli or in response to formerly innocuous stimuli due to unique neurochemical and neurophysiological changes. These changes include acute excitation of peripheral neurons associated with regeneration of axons near the injury site. The causes of injuries leading to neuropathic pain include viral infection as well as trauma. Recently, a highly specific angiotensin II type 2 receptor (AGTR2) antagonist known as EMA401 showed efficacy as a treatment for postherpetic neuralgia in clinical trials. Together with previous immunohistochemical studies of the effects of EMA401 on in vitro neurite outgrowth and the presence of AGTR2 in rodent and human dorsal root ganglion, it ignited interest in AGTR2 as a pharmacological target for neuropathic pain. However, the role of AGTR2 in the modulation of neuropathic pain is not well understood. Despite previous studies, its anatomical expression in dorsal root ganglion and trigeminal ganglion remains uncertain. Additionally, few mechanisms for its modulation of nociceptive transmission have been extensively elucidated. Finally, differential expression of AGTR2 between mouse and human dorsal root ganglion has not been fully explored, especially given the availability of high-throughput expression data. Therefore, this study attempts to develop understanding of the role of AGTR2 in neuropathic pain through bioinformatic analysis of Gene Expression Omnibus (GEO) microarray data for multiple tissues and RNA Seq data acquired for human DRG.
Type:
text; Electronic Dissertation
Keywords:
Medical Pharmacology
Degree Name:
Ph.D.
Degree Level:
doctoral
Degree Program:
Graduate College; Medical Pharmacology
Degree Grantor:
University of Arizona
Advisor:
Vanderah, Todd W.

Full metadata record

DC FieldValue Language
dc.language.isoen_USen
dc.titleBioinformatic Analysis of Angiotensin II Receptor Type 2 Expression and Its Potential Role in Neuropathic Painen_US
dc.creatorShy, Adiaen
dc.contributor.authorShy, Adiaen
dc.date.issued2015en
dc.publisherThe University of Arizona.en
dc.rightsCopyright © is held by the author. Digital access to this material is made possible by the University Libraries, University of Arizona. Further transmission, reproduction or presentation (such as public display or performance) of protected items is prohibited except with permission of the author.en
dc.description.abstractNeuropathic pain is a tremendous medical problem that afflicts millions. It is also distinct from other pain conditions. It persists in the absence of non-noxious stimuli or in response to formerly innocuous stimuli due to unique neurochemical and neurophysiological changes. These changes include acute excitation of peripheral neurons associated with regeneration of axons near the injury site. The causes of injuries leading to neuropathic pain include viral infection as well as trauma. Recently, a highly specific angiotensin II type 2 receptor (AGTR2) antagonist known as EMA401 showed efficacy as a treatment for postherpetic neuralgia in clinical trials. Together with previous immunohistochemical studies of the effects of EMA401 on in vitro neurite outgrowth and the presence of AGTR2 in rodent and human dorsal root ganglion, it ignited interest in AGTR2 as a pharmacological target for neuropathic pain. However, the role of AGTR2 in the modulation of neuropathic pain is not well understood. Despite previous studies, its anatomical expression in dorsal root ganglion and trigeminal ganglion remains uncertain. Additionally, few mechanisms for its modulation of nociceptive transmission have been extensively elucidated. Finally, differential expression of AGTR2 between mouse and human dorsal root ganglion has not been fully explored, especially given the availability of high-throughput expression data. Therefore, this study attempts to develop understanding of the role of AGTR2 in neuropathic pain through bioinformatic analysis of Gene Expression Omnibus (GEO) microarray data for multiple tissues and RNA Seq data acquired for human DRG.en
dc.typetexten
dc.typeElectronic Dissertationen
dc.subjectMedical Pharmacologyen
thesis.degree.namePh.D.en
thesis.degree.leveldoctoralen
thesis.degree.disciplineGraduate Collegeen
thesis.degree.disciplineMedical Pharmacologyen
thesis.degree.grantorUniversity of Arizonaen
dc.contributor.advisorVanderah, Todd W.en
dc.contributor.committeememberFrench, Edward D.en
dc.contributor.committeememberLargent-Milnes, Tally M.en
dc.contributor.committeememberPrice, Theodore J.en
dc.contributor.committeememberVanderah, Todd W.en
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